LC–MS/MS analysis of puerarin and 18β‐glycyrrhetinic acid in human plasma after oral administration of Samso‐eum and its application to pharmacokinetic study

Abstract

The aim of this study was to confirm pharmacokinetic screening of multiple components in healthy Korean subjects after oral administration of Samso‐eum and perform quantitation of active components in the human plasma. Thirteen potential bioactive components [puerarin (PRR), daidzin, nodakenin, ginsenoside Rb1, 18β‐glycyrrhetinic acid (18β‐GTA), 6‐shogaol, naringin, glycyrrhizin, hesperidin, platycodin D, naringenin, hesperetin, and 6‐gingerol] were screened based on literature. The results showed that three analytes (daidzin, naringenin, and hesperetin) were detected in trace amounts. In addition, PRR and 18β‐GTA were detected in human plasma after the oral administration of Samso‐eum. In this study, a liquid chromatography–electrospray ionization‐tandem mass spectrometry method was validated for the simultaneous determination of PRR and 18β‐GTA in human plasma. This was the first study to evaluate pharmacokinetics of PRR and 18β‐GTA after the usual oral dose of Samso‐eum (30 g containing 102.48 mg PRR, 48.18 mg glycyrrhizin) in human subjects.

Natural Products as Modulators of CES1 Activity

“…Similarly, a single oral dose of 75 mg of glycyrrhizin yielded a systemic GLA concentration of 200 ng/ml (Suzuki et al, 2017). GLA ingested as a component of traditional medicines has also been reported (Takeda et al, 1990;Kitagawa et al, 2015a;Sadakane et al, 2015;Lee et al, 2020), with the highest C max observed at 211 ng/ml. Glycyrrhizin has recognized antiviral effects in vitro and has been administered intravenously as a potential therapeutic agent for patients with hepatitis (Kumada, 2002;Manns et al, 2012;Hung et al, 2017).…”

A Highly Sensitive and Rapid Colloidal Gold Immunoassay for Puerarin Detection

“…The content of puerarin in P. lobata should not be less than 2.4%, while P. thomsonii should have a minimum puerarin content of 0.3%. 1 Various analytical methods, such as high-performance thinlayer chromatography (HPTLC), 4 high-performance liquid chromatography (HPLC), 5 liquid chromatography tandem mass spectrometry (LC-MS), 6 and ELISA, 7 have been reported for the detection of puerarin. However, these methods are not suitable for on-site real-time detection due to their long analysis times, high costs, and complex operations.…”