LC-MS/MS analysis of visceral and subcutaneous adipose tissue proteomes in young goats with focus on innate immunity and inflammation related proteins

Restelli, Liliana; Codrea, Marius Cosmin; Savoini, G.; Ceciliani, Fabrizio; Bendixen, Emøke

doi:10.1016/j.jprot.2014.05.027

Cited by 24 publications

(16 citation statements)

References 73 publications

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“…PCOS is a heterogeneous endocrine disorder that causes $10% of infertility in women (Diamanti-Kandarakis, 2008). Interestingly, a proteomic analysis of goat adipose tissue also identified the C1ORF123 homologue as one of the adipokines that may be involved in endocrine function (Restelli et al, 2014). Other proteomics studies have found that the C1ORF123 protein is largely expressed in the hippocampus of people suffering from schizophrenia, bipolar disorder and methamphetamine-induced sensitization of the prefrontal cortex, as well as being a unique protein in the frontal cortex of aged rats associated with slowwave sleep (SWS) (Schubert et al, 2015;Wearne et al, 2015;Vazquez et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Cloning, expression, purification, crystallization and X-ray crystallographic analysis of recombinant human C1ORF123 protein

et al. 2016

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C1ORF123 is a human hypothetical protein found in open reading frame 123 of chromosome 1. The protein belongs to the DUF866 protein family comprising eukaryote-conserved proteins with unknown function. Recent proteomic and bioinformatic analyses identified the presence of C1ORF123 in brain, frontal cortex and synapses, as well as its involvement in endocrine function and polycystic ovary syndrome (PCOS), indicating the importance of its biological role. In order to provide a better understanding of the biological function of the human C1ORF123 protein, the characterization and analysis of recombinant C1ORF123 (rC1ORF123), including overexpression and purification, verification by mass spectrometry and a Western blot using anti-C1ORF123 antibodies, crystallization and X-ray diffraction analysis of the protein crystals, are reported here. The rC1ORF123 protein was crystallized by the hanging-drop vapordiffusion method with a reservoir solution comprised of 20% PEG 3350, 0.2 M magnesium chloride hexahydrate, 0.1 M sodium citrate pH 6.5. The crystals diffracted to 1.9 Å resolution and belonged to an orthorhombic space group with unit-cell parameters a = 59.32, b = 65.35, c = 95.05 Å . The calculated Matthews coefficient (V M ) value of 2.27 Å 3 Da À1 suggests that there are two molecules per asymmetric unit, with an estimated solvent content of 45.7%.

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Section: Introductionmentioning

confidence: 99%

Cloning, expression, purification, crystallization and X-ray crystallographic analysis of recombinant human C1ORF123 protein

et al. 2016

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“…The recent evolution of rapid protein identification technologies has made large-scale proteome analysis possible [31]. One of the most common methods in proteomic analysis is the use of one-dimensional or two-dimensional gel electrophoresis (1-DGE or 2-DGE) followed by enzymatic hydrolysis and mass spectrometry analysis [32–38]. Using this method, several proteomic studies have been conducted with fungi and mushrooms, including Termitomyces heimii [39], Sparassis crispa [35], Hericium erinaceum [35], Arthrobotrys oligospora [40], Metarhizium acridum [30], Agrocybe aegerita [41] and Cordyceps militaris [42].…”

Section: Introductionmentioning

confidence: 99%

Proteome Exploration to Provide a Resource for the Investigation of Ganoderma lucidum

Yin

et al. 2015

PLoS ONE

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Ganoderma lucidum is a basidiomycete white rot fungus that has been used for medicinal purposes worldwide. Although information concerning its genome and transcriptome has recently been reported, relatively little information is available for G. lucidum at the proteomic level. In this study, protein fractions from G. lucidum at three developmental stages (16-day mycelia, and fruiting bodies at 60 and 90 days) were prepared and subjected to LC-MS/MS analysis. A search against the G. lucidum genome database identified 803 proteins. Among these proteins, 61 lignocellulose degrading proteins were detected, most of which (49 proteins) were found in the 90-day fruiting bodies. Fourteen TCA-cycle related proteins, 17 peptidases, two argonaute-like proteins, and two immunomodulatory proteins were also detected. A majority (470) of the 803 proteins had GO annotations and were classified into 36 GO terms, with “binding”, “catalytic activity”, and “hydrolase activity” having high percentages. Additionally, 357 out of the 803 proteins were assigned to at least one COG functional category and grouped into 22 COG classifications. Based on the results from the proteomic and sequence alignment analyses, a potentially new immunomodulatory protein (GL18769) was expressed and shown to have high immunomodulatory activity. In this study, proteomic and biochemical analyses of G. lucidum were performed for the first time, revealing that proteins from this fungus can play significant bioactive roles and providing a new foundation for the further functional investigations that this fungus merits.

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“…The special operations of strong cation exchange liquid chromatography were according to the previous method. 41 The tryptic peptides were fractionated into fractions by high pH reverse-phase HPLC using Agilent 300Extend C18 column (5-μm particles, 4.6-mm ID, and 250-mm length). After eluting, peptides samples were sprayed via a nanospray (PicoTips, silica, no coating, OD360 μm, ID 20 μm, New Objective) directly into the Q-star Elitemass spectrometer (Applied Biosystems, Toronto, Canada).…”

Section: Lc-ms/ms Analysismentioning

confidence: 99%

Reduced delivery of epididymal adipocyte‐derived exosomal resistin is essential for melatonin ameliorating hepatic steatosis in mice

Rong

Feng

Liu

et al. 2019

Journal of Pineal Research

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Adipocyte‐derived exosomes (Exos) serve as bioinformation‐containing messengers in cell‐to‐cell communications, and numerous reports demonstrate that resistin, an adipokine, is strongly associated with hepatic steatosis and other fatty liver diseases, suggesting that adipose dysfunction‐generated altered pattern of exosomal cytokines may contribute to shaping the physiological activities in liver. Admittedly, melatonin‐mediated positive effects on various tissues/organs have been respectively reported, but regulatory mechanisms of melatonin on the crosstalk between adipose tissue and liver have been investigated rarely. Overall, we hypothesize that the crosstalk originating from adipose tissue may be another worthy regulatory pathway for melatonin ameliorating of hepatic steatosis. Here, we first found the amount of adipocyte‐derived exosomal resistin to be significantly decreased by melatonin supplementation. Compared to mice with ExosHFD or Exosresistin treatment, ExosMT remarkably ameliorated hepatic steatosis. Further test demonstrated that resistin was a pivotal cytokine which repressed phosphorylation of 5′ adenosine monophosphate‐activated protein kinase α (pAMPKα Thr172) to trigger endoplasmic reticulum (ER) stress, resulting in hepatic steatosis, whereas ExosMT reversed these risks in hepatocytes. In adipocytes, we identified melatonin to reduce the production of resistin through the brain and muscle arnt‐like protein 1 (Bmal1) transcriptional inhibition. Notably, we also confirmed that melatonin enhanced N6‐Methyladenosine (m6A) RNA demethylation to degrade resistin mRNA in adipocytes. Overall, melatonin decreases traffic volume of adipocyte‐generated exosomal resistin from adipocytes to hepatocytes, which further alleviates ER stress‐induced hepatic steatosis. Our findings illustrate a novel melatonin‐mediated regulatory pathway from adipocytes to hepatocytes, indicating that adipocyte‐derived exosome is a new potential target for treating obesity and related hepatorenal syndrome.

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LC-MS/MS analysis of visceral and subcutaneous adipose tissue proteomes in young goats with focus on innate immunity and inflammation related proteins

Cited by 24 publications

References 73 publications

Cloning, expression, purification, crystallization and X-ray crystallographic analysis of recombinant human C1ORF123 protein

Cloning, expression, purification, crystallization and X-ray crystallographic analysis of recombinant human C1ORF123 protein

Proteome Exploration to Provide a Resource for the Investigation of Ganoderma lucidum

Reduced delivery of epididymal adipocyte‐derived exosomal resistin is essential for melatonin ameliorating hepatic steatosis in mice

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