LC-MS/MS determination of chloramphenicol in food of animal origin in Brazil

Guidi, Letícia Rocha; Silva, Luiza H.M.; Fernandes, Christian; Engeseth, Nicki J.; Glória, Maria Beatriz Abreu

doi:10.4322/sc.2016.010

Cited by 16 publications

(6 citation statements)

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“…Some LC-MS/MS methods have been published concerning the simultaneous determination of CAP in food and feed samples [18][19][20][21][22] with the different chromatographic conditions, longer retention time, and poor recoveries [23]. Although, these methods were reported for quantification of CAP in different sample matrices such as feed, meat, milk, honey, and some biological matrices [24][25][26] they are incompetent to analyze poultry meat. The method we developed and validated is more precise with good selectivity, linearity, precision, and high recovery that met all the criteria of the validation parameters (Table 4).…”

Section: Resultsmentioning

confidence: 99%

Investigation on Chloramphenicol Residues in Poultry Meat in Bangladesh Using a Validated Liquid Chromatography-Tandem Mass Spectrometry

MZ¹,

SMS²,

MM³

2022

austinjanalpharmchem

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Chloramphenicol (CAP) is a broad-spectrum antibiotic widely applied in veterinary practices. Continual use of CAP in livestock production may lead to antibiotic resistance and health-related hazards. In this study, a rapid and highly sensitive analytical method based on ultra-performance liquid chromatographytandem mass spectrometry (UPLC–MS/MS) was developed and validated to detect and quantify there is due of CAP in poultry meat regarding the safety of humans and animals. Total 80 samples of poultry meat were collected from different poultry farms, and poultry meat sellers in three upazilas namely- Sadar, Mirzapur and Ghatail of Tangail district in the Dhaka division. The test analysis was performed using matrix-matched liquid chromatography-mass spectrophotometry. The chromatographic separation of the CA Presidue was carried out at 40°C temperature on a reverse-phase C18 column using a binary gradient pump, and quantification was performed by LC-MS/MS in electrospray mode. Mobile phase constituents were solvent (a) deionized water, and (b) acetonitrile. The flow rate was 0.35 mL/min and the total run time was 5 min. The method was validated in terms of selectivity, linearity, recovery, and precision following the 2021/808/EC guidelines and acceptance criteria were met in all the cases. The relative standard deviation (RSD) for precision was <11%. The linearity of the calibration curves was excellent (R2 >0.999) at concentrations of 0.25, 0.50, 0.75, 1.0, 2.0, and 5.0 μg/kg for matrix-matched CAP standard, and the range of linearity of this method was 0.0-5.0 μg/kg with R2 value greater than 0.99. The decision limit (CC-a), and detection capability (CCβ) were 0.29 μg/ kg, and 0.31 μg/kg respectively, and the recovery percentages ranged from 94 to 100 %. In this study, the levels of CAP residue in tested poultry meat samples were found below the detection limit. The overall parameters of the proposed method met the validation criteria, and the method proved to be suitable for CAP residues determination in poultry meat samples. Thus, this method could be a precise and highly desirable analytical procedure for rapid and routine analysis of CA Presidues in poultry meat, and obtained tested results in this study could be an authentic data to ensure the chloramphenicol free safety poultry meat for human consumption.

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Section: Resultsmentioning

confidence: 99%

Investigation on Chloramphenicol Residues in Poultry Meat in Bangladesh Using a Validated Liquid Chromatography-Tandem Mass Spectrometry

MZ¹,

SMS²,

MM³

2022

austinjanalpharmchem

View full text Add to dashboard Cite

show abstract

“…Due to fatal health issues including bone marrow aplasia, aplastic anemia, and gray baby syndrome, CAP has been banned in food-producing animals in EU, Canada, the United States, and some other countries due to its potential harmful effects on human (Guidi et al, 2015;Samsonova et al, 2012), which explained well that why CAP was not detected in US swine wastewater. However, CAP possesses broad spectrum of activity for disease treatment and is cost-effective, thus it is still commonly used in developing countries.…”

Section: Differences Of Vas In Chinese and Us Swine Wastewatermentioning

confidence: 99%

Veterinary antibiotics in swine and cattle wastewaters of China and the United States: Features and differences

Wan

Liu

2021

Water Environment Research

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Veterinary antibiotics (VAs) have been widely used in livestock for disease prevention, treatment, and growth promotion. This study compared top 20 VAs in Chinese and US swine and cattle wastewater with published literatures. The sulfonamides (SAs) were found to be predominant, accounting for 62% of the top 20 VAs in Chinese swine wastewater, while tetracyclines (TCs) contributed to about 68.7% of the 18 VAs in US swine wastewater. The average concentration of the 20 major VAs in Chinese swine wastewater was estimated to be 1145 μg/L against 253.6 μg/L in the United States. On the other hand, the five major VAs in Chinese cattle wastewater were identified to be oxytetracycline, nafcillin, apramycin, lincomycin, and amikacin, while monensin was found to be dominant in US cattle wastewater. The average concentration of the top 20 VAs in Chinese and US cattle wastewaters were found to be 54.6 and 46.2 μg/L, respectively. These analyses suggested that VAs were probably over-used in Chinese swine industry, eventually causing the development and spreading of antibiotic resistant-bacteria and genes, which should be paid with attention. © 2021 Water Environment Federation • Practitioner points • Major veterinary antibiotics (VAs) in swine and cattle wastewater were identified. • Top 20 VAs in swine and cattle wastewater of China and the United States were compared. • VAs concentration in Chinese swine wastewater was 4.52 times that in the United States. • VAs concentration in Chinese cattle wastewater was 1.18 times that of the United States.

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“…It had been banned in many countries because of the adverse effects on human health [ 25 ]. According to the literature, CAP could still be detected in several food substrates [ 26 ], indicating that it was still being used. As a coating on metal packaging materials for food, bisphenol A (BPA) could migrate into food over time and pose a threat to human health, even under suitable storage conditions.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Magnetic Al-Based Fe3O4@MIL-53 Metal Organic Framework for Capture of Multi-Pollutants Residue in Milk Followed by HPLC-UV

Liu

Wang²,

Ren³

et al. 2022

Molecules

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The efficient capture of multi-pollutant residues in food is vital for food safety monitoring. In this study, in-situ-fabricated magnetic MIL-53(Al) metal organic frameworks (MOFs), with good magnetic responsiveness, were synthesized and applied for the magnetic solid-phase extraction (MSPE) of chloramphenicol, bisphenol A, estradiol, and diethylstilbestrol. Terephthalic acid (H2BDC) organic ligands were pre-coupled on the surface of amino-Fe3O4 composites (H2BDC@Fe3O4). Fe3O4@MIL-53(Al) MOF was fabricated by in-situ hydrothermal polymerization of H2BDC, Al (NO3)3, and H2BDC@Fe3O4. This approach highly increased the stability of the material. The magnetic Fe3O4@MIL-53(Al) MOF-based MSPE was combined with high-performance liquid chromatography-photo diode array detection, to establish a novel sensitive method for analyzing multi-pollutant residues in milk. This method showed good linear correlations, in the range of 0.05–5.00 μg/mL, with good reproducibility. The limit of detection was 0.004–0.108 μg/mL. The presented method was verified using a milk sample, spiked with four pollutants, which enabled high-throughput detection and the accuracies of 88.17–107.58% confirmed its applicability, in real sample analysis.

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LC-MS/MS determination of chloramphenicol in food of animal origin in Brazil

Cited by 16 publications

References 0 publications

Investigation on Chloramphenicol Residues in Poultry Meat in Bangladesh Using a Validated Liquid Chromatography-Tandem Mass Spectrometry

Investigation on Chloramphenicol Residues in Poultry Meat in Bangladesh Using a Validated Liquid Chromatography-Tandem Mass Spectrometry

Veterinary antibiotics in swine and cattle wastewaters of China and the United States: Features and differences

Fabrication of Magnetic Al-Based Fe3O4@MIL-53 Metal Organic Framework for Capture of Multi-Pollutants Residue in Milk Followed by HPLC-UV

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