LC-MS/MS determination of glimepiride in human plasma with a high recovery at picogram scale: Pharmacokinetic study after oral administration

Abstract

Although glimepiride (GLM) is the first-line treatment of Type II diabetes, low extraction recovery is still a significant limitation in previous plasma analysis methods. An optimized solid-phase extraction method of GLM in human plasma with excellent extraction recovery, 100 ± 0.06%, was achieved using liquid chromatography-electrospray ionization tandem mass spectrometry and Gliclazide (GLZ) as an internal standard. GLM was extracted from 100 µL plasma sample using Sep-Pak® vac 1cc (100 mg) C18 column and methylene chloride: methanol (2: 1, v/v) as eluant. Both GLM and GLZ were monitored by a triple quad mass spectrometer applying positive multiple reaction monitoring mode (+MRM). The protonated precursor ions and product ions of GLM and GLZ were m/z 491(352), and m/z 324 (127), respectively. The detection and measurement of low levels of GLM in human plasma reached to picogram range (limit of detection (LOD) = 60 pg/mL, limit of quantification (LOQ) = 200 pg/mL). The method was validated in terms of selectivity, linearity, recovery, accuracy, and precision. The method was successfully applied to the pharmacokinetic study of GLM following oral administration of 1 mg GLM tablets to 12 healthy volunteers.

Transdermal Delivery of Glimepiride: A Novel Approach Using Nanomicelle-Embedded Microneedles

“…The higher MRT of DMN is attributed to slow release of drug from the MN patch, resulting in a prolonged stay within the body and a longer half-life. As high concentration (C max ) of GM was observed with DMN, and GM exhibits >99.5% plasma protein binding, which contributes to its increased T max (4 h) [ 69 ].…”

Quantitative determination of lobeglitazone sulfate and glimepiride in combined tablet by robust high‐performance thin layer chromatographic method

“…Ultraviolet spectrophotometry [15][16][17][18][19][20][21], highperformance liquid chromatography (HPLC) [22][23][24][25][26][27][28][29][30], high-performance thin-layer chromatography (HPTLC) [31][32][33][34][35][36][37], liquid chromatography-mass spectrometry (LC-MS) [38][39][40][41][42][43][44], and gas chromatography-mass spectrometry (GC-MS) [45] are among the analytical approaches stated in the literature for quantifying the concentrations of LBZ and GLM separately or in blend with other substances, in bulk drug samples or pharmaceutical formulations. Only two analytical methods have been reported [22,23] for determining LBZ individually, and these methods cannot be used to estimate the drugs in combined formulations.…”