LC/MS/MS measurement of penicillin G in bovine plasma, urine, and biopsy samples taken from kidneys of standing animals

Heller, David; Smith, Michelle; Chiesa, O. A.

doi:10.1016/j.jchromb.2005.10.025

Cited by 30 publications

(21 citation statements)

References 17 publications

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“…The values found were also compared with similar data found in the literature [25,26,27,28], which are reported in Table 4. Furthermore, several recovery tests were carried out in the analyzed samples using both methods (see Table 5 and Table 6 respectively).…”

Section: Resultssupporting

confidence: 74%

Comparison between a Direct-Flow SPR Immunosensor for Ampicillin and a Competitive Conventional Amperometric Device: Analytical Features and Possible Applications to Real Samples

Tomassetti

Merola

Martini

et al. 2017

Sensors

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In this research, we developed a direct-flow surface plasmon resonance (SPR) immunosensor for ampicillin to perform direct, simple, and fast measurements of this important antibiotic. In order to better evaluate the performance, it was compared with a conventional amperometric immunosensor, working with a competitive format with the aim of finding out experimental real advantages and disadvantages of two respective methods. Results showed that certain analytical features of the new SPR immunodevice, such as the lower limit of detection (LOD) value and the width of the linear range, are poorer than those of a conventional amperometric immunosensor, which adversely affects the application to samples such as natural waters. On the other hand, the SPR immunosensor was more selective to ampicillin, and measurements were more easily and quickly attained compared to those performed with the conventional competitive immunosensor.

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Section: Resultssupporting

confidence: 74%

Comparison between a Direct-Flow SPR Immunosensor for Ampicillin and a Competitive Conventional Amperometric Device: Analytical Features and Possible Applications to Real Samples

Tomassetti

Merola

Martini

et al. 2017

Sensors

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“…Several researchers have also proposed the use of body fluids, such as blood, urine, and kidney exudates, to determine and monitor drug concentrations in live animals and in carcasses of swine (Ashworth et al, 1986), cattle (Lifschitz et al, 1999;Chiesa et al, 2006a,b;Heller et al, 2006), sheep (Bevill et al, 1977a,b), elk (Clark et al, 2004), and poultry (Pant et al, 2005;Haasnoot et al, 2007;Schneider et al, 2007Schneider et al, , 2009Schneider and Lehotay, 2008). Furthermore, this type of analysis is widely used to determine of drug concentrations in humans (Dickson et al, 1994;Rivier, 2000;Hammett-Stabler et al, 2002;Rigamonti et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

The relationship between blood and muscle samples to monitor for residues of the antibiotic enrofloxacin in chickens

et al. 2011

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In 2005, the US Food and Drug Administration withdrew approval for use of enrofloxacin in poultry, thus effectively imposing zero tolerance for residues of this antibiotic in poultry. Conventional residue monitoring for most antibiotics, including enrofloxacin, involves removing poultry carcasses from the processing line and collecting muscle tissues for analysis. Because of the loss of valuable edible products and the difficulties and expense of sampling all the carcasses, only a small portion of carcasses are tested for violative residues. Unlike muscle tissue, blood is readily available from all birds at the beginning of processing and may be used to screen for illegal residues in all poultry carcasses. It is unknown, however, if enrofloxacin concentrations in blood are predictive of muscle concentrations. In an effort to evaluate this relationship, 156 broiler chickens, 5 wk of age, were dosed with either 25 or 50 µg/mL of enrofloxacin for 3 or 7 d, respectively, in the drinking water. Blood and muscle samples were collected at 0, 1, 3, 6, 12, and 24 h (n = 6 birds/group) during the first dosing day, every 48 h during the dosing period, and every 12 h during the withdrawal period for up to 60 h after withdrawal. Enrofloxacin residues were determined in all blood and tissue samples during the dosing periods and in most of the withdrawal period for both doses. These results support the potential to use blood to screen for illegal enrofloxacin residues in edible poultry tissues in an effort to protect the human food supply.

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“…Most probably, the decline in the concentration is not due to metabolism by cells but due to the degradation of penicillin G. In the literature, highly variable exponential decays are reported. 20,21 Temperature is an important parameter: at 5°C, the remaining concentration left after 1 month in bovine serum 20 is approximately 25%. In contrast, at 37°C, a penicillin G half-life of 5 days was noted, 21 which would result in a concentration of 25% after only 10 days.…”

Section: Discussionmentioning

confidence: 99%

Time Course of Antibiotic and Antifungal Concentrations in Corneal Organ Culture

Seiler

Tschopp

Zimmerli

et al. 2016

Cornea

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Purpose: Contamination with bacteria and/or fungi is a serious complication in organ-cultured corneas. Hence, antibiotic and antifungal agents are added to the culture medium. The concentration of different antimicrobial and antifungal additives to the media over time has so far not been investigated in detail and is the aim of this study.Methods: Nine human fresh corneoscleral discs were stored in corneal culture medium consisting of 2% fetal bovine serum and minimal essential medium. In addition, the culture medium contained 1200 mg/mL penicillin G, 25 mg/mL amphotericin B, 120 mg/mL streptomycin, and 100 mg/mL voriconazole. The concentration of amphotericin B used was 10 times higher than in clinical routine to facilitate its detection. The cultures were kept at 37°C for 28 days. At days 0, 7, 14, 21, and 28, samples of the culture medium were harvested for analysis of antimicrobial concentrations by liquid chromatography and electrospray ionization tandem mass spectrometry.Results: During corneal storage, the concentration of all antibiotics and antifungal agents declined significantly. By day 28, penicillin G was reduced to 14% of the original concentration. Amphotericin B and streptomycin retained approximately 60% of the original concentration to the end of the experiment and voriconazole maintained stable concentrations after an initial decline to approximately 80% at 7 days.Conclusions: Throughout the entire storage period, the concentrations of penicillin G, streptomycin, and voriconazole exceeded the minimum inhibitory concentrations of all common contaminants, obviating the need for a change of the medium for antimicrobial reasons. Based on the minimum inhibitory concentrations and our findings, the initial concentration of amphotericin B should be raised to 5 mg/mL. Warm preservation at 31 to 37°C is more commonly used in European eye banks: corneas can be stored up to 4 weeks in organ culture medium consisting of minimum essential medium with the addition of 2% fetal bovine serum.2-6 Antibiotics and antifungal agents are added to the organ culture media to kill contaminants and to avoid iatrogenic infections at transplantation. Despite this prophylaxis, severe complications like endophthalmitis can still occur. 7,8 Little is known about the decay characteristics and efficacy over time of antimicrobial agents kept at body temperature. Therefore, we studied the concentration of the most commonly used antimicrobial and antifungal additives over time under the standard conditions established at the local eye bank at the Department of Ophthalmology, University Hospital in Bern. METHODS Sample PreparationNine human corneoscleral discs unsuitable for transplantation were excised under sterile conditions from intact globes not later than 6 hours post mortem and stored each in a corneal culture medium consisting of 2% fetal bovine serum and minimal essential medium (Sigma Aldrich, St Louis, MO) following the standard protocol of the eye bank of the University Hospital of Bern. The culture medium contained 1...

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LC/MS/MS measurement of penicillin G in bovine plasma, urine, and biopsy samples taken from kidneys of standing animals

Cited by 30 publications

References 17 publications

Comparison between a Direct-Flow SPR Immunosensor for Ampicillin and a Competitive Conventional Amperometric Device: Analytical Features and Possible Applications to Real Samples

Comparison between a Direct-Flow SPR Immunosensor for Ampicillin and a Competitive Conventional Amperometric Device: Analytical Features and Possible Applications to Real Samples

The relationship between blood and muscle samples to monitor for residues of the antibiotic enrofloxacin in chickens

Time Course of Antibiotic and Antifungal Concentrations in Corneal Organ Culture

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