LC‐MS‐MS Method for Determination of Metolazone in Human Plasma

M.N.Roy, Shikha; Mangaonkar, Kiran V.; Yetal, Santosh M.; Joshi, Santosh S.

doi:10.1155/2008/425974

Cited by 12 publications

(6 citation statements)

References 11 publications

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“…These methods include HPLC for the determination of MET either alone or with furosemide . Also, liquid chromatography‐tandem mass spectrometry (LC‐MS) has been reported for the analysis of MET in human pasma and blood. Regarding LOS, various liquid chromatography methods have been reported for its determination, individually or in combination with other drugs.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of metolazone and losartan potassium in their binary mixtures using high‐performance liquid chromatography with fluorimetric detection: application to combined tablets and spiked human plasma

et al. 2013

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A new, specific and sensitive reversed-phase high-performance liquid chromatography method was developed for the simultaneous determination of metolazone (MET) and losartan potassium (LOS). Good chromatographic separation was achieved within 6.0 min on a 150 × 4.6 mm i.d., 5 µm Waters, Ireland and ProDIGY 5 ODS 3 100 A column. A mobile phase containing a mixture of methanol and 0.02 M phosphate buffer (65:35, v/v) at pH 3.0 was used. The analysis was performed at a flow rate of 1 mL/min with fluorescence detection at 410 nm after excitation at 230 nm. Aspirin (ASP) was used as an internal standard. The proposed method was rectilinear over 2.0-40.0 (MET) and 40.0-800.0 ng/mL (LOS), with limits of detection of 0.22 and 4.52 ng/mL and limits of quantification of 0.68 and 13.70 ng/mL for MET and LOS, respectively. The method was successfully applied for the simultaneous analysis of the studied drugs in their laboratory-prepared mixtures, single tablets and co-formulated tablets. Moreover, the method was applied to an in vitro drug release (dissolution) test. The method was further extended to the determination of LOS in spiked human plasma. Statistical evaluation and comparison of data obtained using the proposed and comparison methods revealed no significant difference between the two methods in addition to good accuracy and precision for the proposed method.

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Section: Introductionmentioning

confidence: 99%

Simultaneous determination of metolazone and losartan potassium in their binary mixtures using high‐performance liquid chromatography with fluorimetric detection: application to combined tablets and spiked human plasma

et al. 2013

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“…Literature review reveals that methods have been reported for analysis of Losartan potassium and Metolazone, HPLC method for determination of Losartan potassium in combination with other drugs [ 4 – 6 ] and few bioanalytical methods are also reported [ 7 – 10 ]. RP-HPLC method for determination of Metolazone [ 11 ] and few bioanalytical methods are also reported [ 12 – 14 ].…”

Section: Introductionmentioning

confidence: 99%

Validated RP-HPLC Method for Simultaneous Quantitation of Losartan Potassium and Metolazone in Bulk Drug and Formulation

Dubey

Bhusari²,

Dhaneshwar³

2011

Sci. Pharm.

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A HPLC method has been described for simultaneous determination of Losartan potassium and Metolazone in formulation. This method is based on a HPLC separation of the two drugs on the Thermo Hypersil BDS–C18 (250 mm × 4.6 mm, 5.0 μm) with isocratic conditions and a simple mobile phase containing acetonitrile:water (60:40) at a flow rate of 0.8 mL/min using UV detection at 237 nm. This method has been applied to a marketed formulation without interference of excipients. The linear regression analysis data for the calibration plots showed a good linear relationship over the concentration range of 2–12 μg/mL for Losartan potassium and 0.2–1.2 μg/mL for Metolazone, respectively. The method was validated for precision, robustness and recovery. Statistical analysis showed that the method is repeatable and selective for the estimation of Losartan potassium and Metolazone.

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“…Metzn is an oral diuretic drug, and is mainly used to cure congestive heart failure and high blood pressure. It is frequently used together with loop diuretics such as furosemide or bumetanide 1 . Metzn indirectly reduces the amount of water reabsorbed in to the bloodstream by the kidney, so that blood volume decreases and urine volume increases.…”

Section: Introductionmentioning

confidence: 99%

Separation of Enantiomers of Metolazone by Thin Layer Chromatography Using a Chiral Selector

Bala,

Sethi,

Sharma

2023

J. Adv. Zool.

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Enantiomeric separation of (RS)-Metolazone has been achieved by thin-layer chromatography (TLC) using both direct modes.  cyclodextrin, containing hydroxylic group, was utilized as the chiral selector. For enantioseparation, cyclodextrin was used as a chiral additive in the stationary phase in a non-covalent mode and there was no chiral additive in the mobile phase; the native enantiomers were isolated and characterized. Cyclodextrin was also added to mobile phase and there was no chiral selector in the stationary phase. The spots were then isolated and characterized. The effect of the composition of the mobile phase, pH, and temperature was studied for the optimization of successful separation conditions. The spots were located in an iodine chamber.

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LC‐MS‐MS Method for Determination of Metolazone in Human Plasma

Cited by 12 publications

References 11 publications

Simultaneous determination of metolazone and losartan potassium in their binary mixtures using high‐performance liquid chromatography with fluorimetric detection: application to combined tablets and spiked human plasma

Simultaneous determination of metolazone and losartan potassium in their binary mixtures using high‐performance liquid chromatography with fluorimetric detection: application to combined tablets and spiked human plasma

Validated RP-HPLC Method for Simultaneous Quantitation of Losartan Potassium and Metolazone in Bulk Drug and Formulation

Separation of Enantiomers of Metolazone by Thin Layer Chromatography Using a Chiral Selector

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