LC-MS/MS signal suppression effects in the analysis of pesticides in complex environmental matrices

Choi, Bernard K.; Hercules, David M.; Gusev, Arkady I.

doi:10.1007/s002160000661

Cited by 115 publications

(85 citation statements)

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“…Post column infusion experiments were conducted to determine ion suppression/enhancement effects using the approach described by Choi et al [36]. The experimental setup used in these experiments involved the infusion of a standard during the analysis of a blank matrix solution.…”

Section: Ion Suppression Post Column Infusion Assessmentmentioning

confidence: 99%

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Development of an LC-MS/MS method for the analysis of serotonin and related compounds in urine and the identification of a potential biomarker for attention deficit hyperactivity/hyperkinetic disorder

et al. 2011

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Serotonin is a major neurotransmitter and affects various functions both in the brain and in the rest of the body. It has been demonstrated that altered serotinergic function is implicated in various psychiatric disorders including depression and schizophrenia. Serotonin has also been implicated along with dopamine in attention deficithyperkinetic disorder (AD-HKD). This study provides a versatile validated method for the analysis of serotonin, hydroxyindole acetic acid and dopamine in urine using LC-MS/MS. This method was then used to quantify these analytes in a test group of 17 children diagnosed with severe AD-HKD. This group was compared to a matched control group to investigate the possibility that one of these compounds may be a potential biomarker for this condition. The developed method provided good linear calibration curves for the multiplex assay of analytes in urine (0.05-3.27 nmol/L; R 2 ≥0.9977). Acceptable inter-day repeatability was achieved for all analytes with RSD values (n=9) ranging from 1.1% to 9.3% over a concentration range of 0.11-3.27 μmol/L in urine. Excellent limits of detection (LOD) and limits of quantitation (LOQ) were achieved with LODs of 8.8-18.2 nmol/L and the LOQs of 29.4-55.7 nmol/L for analytes in urine. Recoveries were in the ranges of 98-104%, 100-106% and 91-107% for serotonin, 5-HIAA and dopamine, respectively. An appropriate sample clean-up procedure for urine was developed to ensure efficient recovery and reproducibility on analysis. Evaluation of matrix effects was also carried out and the influence of ion suppression on analytical results reported. Confirmatory analysis was carried out on a linear trap quadrupole-Orbitrap mass spectrometer to obtain high mass accuracy data of the target analytes in the clinical samples.

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Section: Ion Suppression Post Column Infusion Assessmentmentioning

confidence: 99%

“…Continuous post column infusion of a standard solution ensures that all matrix components in a sample that elute from the column are ionised along with the analyte [46]. This allows areas of ion suppression or enhancement to be easily identified.…”

Section: Matrix Effectsmentioning

confidence: 99%

Development of an LC-MS/MS method for the analysis of serotonin and related compounds in urine and the identification of a potential biomarker for attention deficit hyperactivity/hyperkinetic disorder

et al. 2011

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“…According to Benijts et al [75] the best way to tackle matrix effect is to use appropriate internal standards, being preferred an isotopically labeled one, even though they are not regularly available and alternatively structural analogues are used [76]. On the other hand, the internal standard had to elute close to the compound of interest, showing similar behavior in the detector, in such a way that more than one of them should be used [77].…”

Section: Variations In the Analytical Responsementioning

confidence: 99%

Chemometric strategies for enhancing the chromatographic methodologies with second-order data analysis of compounds when peaks are overlapped

Goicoechea

Culzoni

García

et al. 2011

Talanta

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This overview covers the different chemometric strategies linked to chromatographic methodologies that have been used and presented in the recent literature to cope with problems related to incomplete separation, the presence of unexpected components in the sample, matrix effect and changes in the analytical signal due to pre-treatment of sample.Among the different chemometric strategies it focuses on pre-treatment of data to correct background and time shift of chromatographic peaks and the use of second-order algorithms to cope with overlapping peaks from analytes or from analytes and interferences in liquid chromatography coupled to diode array, fast-scanning fluorescence spectroscopy and mass spectrometry detectors.Finally the review presents the strategies used to deal with changes in the analytical response as result of matrix effect in liquid and gas chromatography, as well as the use of standardization strategies to correct modifications in the analytical signal as a consequence of sample pre-treatment in liquid chromatography.

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“…These experiments were performed in triplicate at three concentration levels. Ion suppression experiments for the internal standards were performed in a similar way [20].…”

Section: Ion Suppressionmentioning

confidence: 99%

“…The most abundant fragments observed in the fragmentation of the m/z of 271 (EO9; Fig. 4 and EO9-Cl [19,20]. For EO9-d3 and EO5a-d4 similar transitions as for EO9 and EO5a, respectively, were selected and optimized.…”

Section: Mass Spectrometrymentioning

confidence: 99%

Simultaneous Quantitative Analysis of EO9 (Apaziquone) and its Conversion Products EO5a and EO9-Cl in Human and Dog Urine by High-Performance Liquid Chromatography Coupled with Electrospray Tandem Mass Spectrometry

Vainchtein¹,

Rosing²,

Mirejovsky³

et al. 2009

TOCBMJ

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Abstract:A sensitive and specific LC-MS/MS assay for the quantitative determination of anticancer agent EO9 and its conversion products EO5a and EO9-Cl in human and dog urine is presented. A 20-μL-urine aliquot was spiked with a mixture of deuterated internal standards EO9-d3 and EO5a-d4 and diluted with 180 L 0.1 M ammonium acetatemethanol (7:3, v/v). Next, 25 μL-volumes were injected onto the HPLC system. Separation was achieved on a 150 2.1 mm C18 column using an alkaline eluent (1 mM ammonium hydroxide -methanol (gradient system)). Detection was executed by positive ion electrospray followed by tandem mass spectrometry. The assay quantifies a range from 0.1 g/mL to 50 g/mL for EO9, from 0.2 g/mL to 50 g/mL for EO5a and 0.1 g/mL to 4 g/mL for EO9-Cl using 20 μL of stabilized urine samples. Validation results demonstrate that EO9, EO5a and EO9-Cl concentrations can be accurately and precisely quantified in human and dog urine. This assay is used now to support pre-clinical and clinical pharmacologic studies with intravesically administered EO9.

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LC-MS/MS signal suppression effects in the analysis of pesticides in complex environmental matrices

Cited by 115 publications

References 0 publications

Development of an LC-MS/MS method for the analysis of serotonin and related compounds in urine and the identification of a potential biomarker for attention deficit hyperactivity/hyperkinetic disorder

Development of an LC-MS/MS method for the analysis of serotonin and related compounds in urine and the identification of a potential biomarker for attention deficit hyperactivity/hyperkinetic disorder

Chemometric strategies for enhancing the chromatographic methodologies with second-order data analysis of compounds when peaks are overlapped

Simultaneous Quantitative Analysis of EO9 (Apaziquone) and its Conversion Products EO5a and EO9-Cl in Human and Dog Urine by High-Performance Liquid Chromatography Coupled with Electrospray Tandem Mass Spectrometry

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