LC-Q/TOF-HRMS and NMR Based Structural Characterization of the Major Photodegradation Impurity of Difluprednate

Sharma, Nitish; Giri, Shivraj; Sahu, Amit Kumar; More, Sidheshwari; Sengupta, Pinaki; Shah, Ravi P.

doi:10.1007/s10337-022-04159-7

Cited by 2 publications

(1 citation statement)

References 16 publications

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“…In particular, DP‐II involved the same mechanism as DP‐I, in which the chloride at position 21 in the side chain was eliminated through hydrolytic dehalogenation. DP‐III was formed through a lumisteroid mechanism, a process that has been previously reported in the photolytic degradation of difluprednate (Sharma et al, 2022). A well‐known C‐1–C‐11 epoxy product was generated as DP‐IV through a rearrangement reaction (Weizel et al, 2020).…”

Section: Resultsmentioning

confidence: 79%

Identification and structural characterization of major stress degradation products of halcinonide by liquid chromatography–high‐resolution mass spectrometry

Giri,

Badgujar,

Paritala

et al. 2023

Biomedical Chromatography

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Halcinonide is a topical corticosteroid approved by the US Food and Drug Administration (FDA), known for its anti‐inflammatory and antipruritic properties. The therapeutic benefits of halcinonide have rendered it an effective treatment regimen for various dermatological conditions such as psoriasis, dermatitis, and eczema. However, stability of the drug substance is a prerequisite in determining the therapeutic efficacy and plays a crucial role during formulation development and long‐term storage. As corticosteroids are highly susceptible to degradation, the current study aims to expose halcinonide to different stress conditions and understand its stability behavior. An HPLC method was developed for the separation of halcinonide and its degradation products. Separation was accomplished in gradient mode using an Eclipse Plus C18 column (250 × 4.5 mm, 5 μm) with ammonium formate (10 mM, pH 6.5) and acetonitrile as the mobile phases. LC–Q‐TOF/MS/MS studies were conducted on halcinonide, which led to the identification of degraded products using optimized mass parameters. A potential mechanistic degradation pathway for halcinonide, along with the major identified degradation products has been established. The chromatographic method that was developed has been validated in compliance with the Q2(R1) guideline of the International Council for Harmonization. ProTox‐II was used to perform in silico toxicity studies in order to evaluate the toxicity potential of both halcinonide and the identified degradation products.

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Section: Resultsmentioning

confidence: 79%