LC-tandem mass spectrometry as a screening tool for multiple detection of allergenic ingredients in complex foods

Monaci, Linda; Pilolli, Rosa; Angelis, Elisabetta De; Carone, Rossella; Pascale, Michelangelo

doi:10.21014/acta_imeko.v5i1.278

Cited by 12 publications

(3 citation statements)

References 18 publications

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“…The quantification of the method was reported to be comprised between 0.31 and 40 mg/kg total peanut, while variable recovery values ranged from 51.1% to 104.2% for peptide DLAFPGSGEQVEK and from 54.4 to 113.0% for peptide IFLAGDKDNVIDQIEK were obtained depending on the wheat-based matrix considered [ 217 ]. In the recent years, high-resolution tandem MS based on Orbitrap mass analyzer for multiple analysis of allergen proteins in food products, including those from peanuts, have been reported [ 20 , 50 , 92 , 112 , 114 , 218 , 219 ]. By exploiting a stand-alone Obitrap-based analyzer, Monaci and colleagues detected and quantified traces of peanut proteins in a flour obtained from a mixture of nuts (hazelnuts, pistachios, almonds and walnuts).…”

Section: Overview Of Nuts and Peanut Allergens Composition And Analyt...mentioning

confidence: 99%

Tree Nuts and Peanuts as a Source of Beneficial Compounds and a Threat for Allergic Consumers: Overview on Methods for Their Detection in Complex Food Products

Luparelli

Losito

Angelis

et al. 2022

Foods

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Consumption of tree nuts and peanuts has considerably increased over the last decades due to their nutritional composition and the content of beneficial compounds. On the other hand, such widespread consumption worldwide has also generated a growing incidence of allergy in the sensitive population. Allergy to nuts and peanuts represents a global relevant problem, especially due to the risk of the ingestion of hidden allergens as a result of cross-contamination between production lines at industrial level occurring during food manufacturing. The present review provides insights on peanuts, almonds, and four nut allergens—namely hazelnuts, walnuts, cashew, and pistachios—that are likely to cross-contaminate different food commodities. The paper aims at covering both the biochemical aspect linked to the identified allergenic proteins for each allergen category and the different methodological approaches developed for allergens detection and identification. Attention has been also paid to mass spectrometry methods and to current efforts of the scientific community to identify a harmonized approach for allergens quantification through the detection of allergen markers.

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Section: Overview Of Nuts and Peanut Allergens Composition And Analyt...mentioning

confidence: 99%

Tree Nuts and Peanuts as a Source of Beneficial Compounds and a Threat for Allergic Consumers: Overview on Methods for Their Detection in Complex Food Products

Luparelli

Losito

Angelis

et al. 2022

Foods

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“…The objective of discovery proteomics analysis was to investigate the protein and peptide profiles of different soy ingredients and use the results to inform selection of tryptic peptide markers for future targeted analysis. Although there has been some similar research performed by other authors, most of these studies were conducted on the whole soybean or on limited types of soy ingredients, ,,, which does not necessarily represent the different types of commercially available processed soy ingredients. Therefore, a discovery analysis was performed on six different soy ingredients to investigate the protein profiles and select potential peptide markers representative to all six ingredients.…”

Section: Resultsmentioning

confidence: 99%

Detection of Six Commercially Processed Soy Ingredients in an Incurred Food Matrix Using Parallel Reaction Monitoring

2019

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Soybeans are one of the major allergenic foods in many countries. Soybeans are commonly processed into different types of soy ingredients to achieve the desired properties. The processing, however, may affect the protein profiles and protein structure, thus affecting the detection of soy proteins. Mass spectrometry (MS) is a potential alternative to the traditional immunoassays for the detection of soy-derived ingredients in foods. This study aims to develop a liquid chromatography−tandem MS method that uniformly detects different types of soy-derived ingredients. Target peptides applicable to the detection of six commercial soy ingredients were identified based on the results of MS label-free quantification and a set of selection criteria. The results indicated that soy ingredient processing can result in different protein profiles. A total of six soy ingredients were then individually incurred into cookie matrices at different levels. Sample preparation methods were optimized, and a distinct improvement in peptide performance was observed after optimization. Cookies and dough incurred with different soy ingredients at 100 ppm total soy protein showed a similar level of peptide recovery (90% mean signal relative to unroasted soy flour), demonstrating the ability of the MS method to detect processed soy ingredients in a uniform manner.

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“…LC-MS has proven to be a bench mark technique for the sensitive quantitative analysis of milk protein allergen in a different food matrices and offers high accuracy and reproducibility (Monaci et al, 2016;Parker et al, 2015;Tolin et al, 2012). Although mass spectrometry based methods offer superior sensitivity compared to other analytical methods available for analysis of milk allergens, analysis still needs to be carried out in a dedicated lab by trained personnel.…”

Section: Introductionmentioning

confidence: 99%

An SPR based sensor for allergens detection

Ashley

Piekarska

Segers

et al. 2017

Biosensors and Bioelectronics

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A simple, sensitive and label-free optical sensor method was developed for allergens analysis using α-casein as the biomarker for cow's milk detection, to be used directly in final rinse samples of cleaning in place systems (CIP) of food manufacturers. A Surface Plasmon Resonance (SPR) sensor chip consisting of four sensing arrays enabling the measurement of samples and control binding events simultaneously on the sensor surface was employed in this work. SPR offers several advantages in terms of label free detection, real time measurements and superior sensitivity when compared to ELISA based techniques. The gold sensor chip was used to immobilise α-casein-polyclonal antibody using EDC/NHS coupling procedure. The performance of the assay and the sensor was first optimised and characterised in pure buffer conditions giving a detection limit of 58ngmL as a direct binding assay. The assay sensitivity can be further improved by using sandwich assay format and amplified with nanoparticles. However, at this stage this is not required as the detection limit achieved exceeded the required allergens detection levels of 2µgmL for α-S1-casein. The sensor demonstrated good selectivity towards the α-casein as the target analyte and adequate recoveries from CIP final rinse wash samples. The sensor would be useful tool for monitoring allergen levels after cleaning procedures, providing additional data that may better inform upon wider food allergen risk management decision(s) that are made by food manufacturer. In particular, this sensor could potentially help validate or optimise cleaning practices for a given food manufacturing process.

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LC-tandem mass spectrometry as a screening tool for multiple detection of allergenic ingredients in complex foods

Cited by 12 publications

References 18 publications

Tree Nuts and Peanuts as a Source of Beneficial Compounds and a Threat for Allergic Consumers: Overview on Methods for Their Detection in Complex Food Products

Tree Nuts and Peanuts as a Source of Beneficial Compounds and a Threat for Allergic Consumers: Overview on Methods for Their Detection in Complex Food Products

Detection of Six Commercially Processed Soy Ingredients in an Incurred Food Matrix Using Parallel Reaction Monitoring

An SPR based sensor for allergens detection

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