LDL-receptor mRNA expression in liver regeneration after partial hepatectomy

Yamamoto, Koichiro; Fukushima, N; Ozaki, Iwata; Motomura, M.; Yanagita, Teruyoshi; Sakai, Takahiro

doi:10.1007/bf02776832

Cited by 2 publications

(1 citation statement)

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“…The demand for membrane formation in regenerating livers and the decrease of cholesterol synthesis is compensated by the increase in low-density lipoprotein uptake (31) and the decrease in biliary cholesterol secretion (32) and bile acid synthesis (33). However, the balance of cholesterol homeostasis is not maintained in the early stage of liver regeneration.…”

Section: Discussionmentioning

confidence: 99%

Zonation of cholesterol and glycerolipid synthesis in regenerating rat livers

Cheng

1993

Hepatology

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Hepatic zonation of cholesterol and glycerolipid synthesis was investigated in regenerating rat livers 24 hr after partial hepatectomy. Tritiated acetate and [U-14C]glycerol were injected into rats' peritoneal cavities for a short-term labeling study. Periportal and perivenous hepatocytes were isolated by digitonin collagenase perfusion. Cholesterol synthesis was significantly higher in periportal hepatocytes of the sham-operated livers (periportal/perivenous = 1.67; p < 0.05). Twenty-four hours after partial hepatectomy, cholesterol synthesis was selectively decreased by 40% (p < 0.01) in periportal hepatocytes. Consequently, hepatic zonation of cholesterol synthesis was abolished in regenerating livers. To study the cholesterol homeostasis on a long-term basis, we substituted deuterated water (25% enriched) for drinking water for 5 days to label newly synthesized cholesterol in a steady state. This procedure clearly demonstrated the net negative cholesterol balance 24 hr after partial hepatectomy. However, the newly synthesized cholesterol contributed equally to the cellular cholesterol pool in both zones. The synthesis of glycerolipids, whether measured from tritiated acetate or [U-14C]glycerol, was significantly increased without apparent zonation in the regenerating livers (twofold increase in phospholipid, and threefold to sevenfold increase in triacylglycerol). We concluded that hepatic zonation of cholesterol synthesis is caused by higher de novo synthesis in periportal hepatocytes, which is abolished in regenerating livers. No zonation of glycerolipid synthesis exists in normal and regenerating livers.

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Section: Discussionmentioning

confidence: 99%