LDL receptor related protein 1 is an adverse prognostic biomarker that correlates with stromal remodeling and macrophages infiltration in bladder cancer

Du, Yueyao; Liu, YiZheng; Cao, Jin; Jiang, Xiang; Wang, Yi; Yu, Jian; Wang, Bo; Wang, XiZhi; Xue, Boxin

doi:10.3389/fimmu.2023.1113756

Cited by 5 publications

(2 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The inhibitory concentration (IC 50 ) was evaluated to determine drug sensitivity. Moreover, IMvigor210 was used to predict the correlation between the immunotherapy response and risk score ( 65 ).…”

Section: Methodsmentioning

confidence: 99%

Construction and validation of a signature for T cell-positive regulators related to tumor microenvironment and heterogeneity of gastric cancer

Guo,

Zhang,

Cen

et al. 2023

Front. Immunol.

View full text Add to dashboard Cite

BackgroundPositive regulators of T cell function play a vital role in the proliferation and differentiation of T cells. However, their functions in gastric cancer have not been explored so far.MethodsThe TCGA-STAD dataset was utilized to perform consensus clustering in order to identify subtypes related to T cell-positive regulators. The prognostic differentially expressed genes of these subtypes were identified using the least absolute shrinkage and selection operator (LASSO) regression analysis. To validate the robustness of the identified signature, verification analyses were conducted across the TCGA-train, TCGA-test, and GEO datasets. Additionally, a nomogram was constructed to enhance the clinical efficacy of this predictive tool. Transwell migration, colony formation, and T cell co-culture assays were used to confirm the function of the signature gene in gastric cancer and its influence on T cell activation.ResultsTwo distinct clusters of gastric cancer, related to T cell-positive regulation, were discovered through the analysis of gene expression. These clusters exhibited notable disparities in terms of survival rates (P = 0.028), immune cell infiltration (P< 0.05), and response to immunotherapy (P< 0.05). Furthermore, a 14-gene signature was developed to classify gastric cancer into low- and high-risk groups, revealing significant differences in survival rates, tumor microenvironment, tumor mutation burden, and drug sensitivity (P< 0.05). Lastly, a comprehensive nomogram model was constructed, incorporating risk factors and various clinical characteristics, to provide an optimal predictive tool. Additionally, an assessment was conducted on the purported molecular functionalities of low- and high-risk gastric cancers. Suppression of DNAAF3 has been observed to diminish the migratory and proliferative capabilities of gastric cancer, as well as attenuate the activation of T cells induced by gastric cancer within the tumor microenvironment.ConclusionWe identified an ideal prognostic signature based on the positive regulators of T cell function in this study.

show abstract

Section: Methodsmentioning

confidence: 99%

Construction and validation of a signature for T cell-positive regulators related to tumor microenvironment and heterogeneity of gastric cancer

Guo,

Zhang,

Cen

et al. 2023

Front. Immunol.

View full text Add to dashboard Cite

show abstract

“…APOF is not only associated with lipid metabolism but also potentially with glucose metabolism and has recently been identified as a circulating biomarker associated with the risk of type 2 diabetes [ 9 ]. Given the established association between lipid metabolism and disease risk for various types of cancer, for example, prostate cancer and bladder cancer [ 10 , 11 ], this is not difficult to understand that the expression level of APOF has been found to be helpful in colorectal cancer (CRC) and cholangiocarcinoma (CHOL) screening [ 12 , 13 ], predicting prognosis in hepatocellular carcinoma (LIHC) [ 14 ], and HPV status in oropharyngeal squamous cell carcinoma in previous studies [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

A pan-cancer analysis of the oncogenic and immunological roles of apolipoprotein F (APOF) in human cancer

Shi

Feng

et al. 2023

Eur J Med Res

View full text Add to dashboard Cite

Background Apolipoprotein F (APOF) has been less studied in cancers. Thus, we aimed to perform a pan-cancer analysis of the oncogenic and immunological effects of APOF on human cancer. Methods A standardized TCGA pan-cancer dataset was downloaded. Differential expression, clinical prognosis, genetic mutations, immune infiltration, epigenetic modifications, tumor stemness and heterogeneity were analyzed. We conducted all analyses through software R (version 3.6.3) and its suitable packages. Results Overall, we found that the common cancers differentially expressed between tumor and normal samples and prognostic-associated were BRCA, PRAD, KIRP, and LIHC in terms of overall survival (OS), disease-free survival (DFS) and progression-free survival (PFS). The pan-cancer Spearman analysis showed that the mRNA expression of APOF was negatively correlated with four tumor stemness indexes (DMPss, DNAss, ENHss, and EREG-METHss) with statistical significance for PRAD and was positively correlated for LIHC. In terms of BRCA and PRAD patients, we found negative correlation of APOF with TMB, MSI, neo, HRD and LOH. The mutation frequencies of BRCA and LIHC were 0.3%. APOF expression was negatively correlated with immune infiltration and positively correlated with tumor purity for PRAD patients. The mRNA expression of APOF was negatively associated with most TILs for LIHC, B cells, CD4+ T cells, neutrophils, macrophages and dendritic cells, but was positively associated with CD8+ T cells. Conclusions Our pan-cancer study offered a relatively comprehensive understanding of the roles of APOF on BRCA, PRAD, KIRP, and LIHC.

show abstract

Loss of LRP1 Promotes Hepatocellular Carcinoma Progression via UFL1‐Mediated Activation of NF‐κB Signaling

Guo,

Yang,

Liu

et al. 2024

Advanced Science

View full text Add to dashboard Cite

Low‐density lipoprotein receptor‐related protein‐1 (LRP1) is thought to be correlated with hepatocellular carcinoma (HCC) invasion and metastasis. However, the precise mechanism through which LRP1 contributes to HCC progression remains unclear. Here, lower LRP1 levels are associated with malignant progression, and poor prognosis in patients with HCC is shown. LRP1 knockdown enhances the tumorigenicity of HCC cells in vitro and in vivo, whereas overexpression of either LRP1 or its β‐chain has the opposite effect. Mechanistically, LRP1 knockdown promotes the binding of ubiquitin‐like modifier 1 ligating enzyme 1 (UFL1) to OGA and accelerates ubiquitin‐mediated OGA degradation, leading to increased O‐GlcNAcylation of nuclear factor‐kappa B (NF‐κB) and subsequent inhibition of pro‐apoptotic gene expression. Conversely, exogenously expressed truncated β‐chain (β∆) stabilizes OGA by disrupting the association between UFL1 and OGA, consequently abolishing the anti‐apoptotic effects of O‐GlcNAcylated NF‐κB. The findings identify LRP1, particularly its β‐chain, as a novel upstream control factor that facilitates the stabilization of the OGA protein, thereby suppressing NF‐κB signaling and attenuating HCC progression, thus suggesting a novel therapeutic strategy for HCC.

show abstract

LDL receptor related protein 1 is an adverse prognostic biomarker that correlates with stromal remodeling and macrophages infiltration in bladder cancer

Cited by 5 publications

References 34 publications

Construction and validation of a signature for T cell-positive regulators related to tumor microenvironment and heterogeneity of gastric cancer

Construction and validation of a signature for T cell-positive regulators related to tumor microenvironment and heterogeneity of gastric cancer

A pan-cancer analysis of the oncogenic and immunological roles of apolipoprotein F (APOF) in human cancer

Loss of LRP1 Promotes Hepatocellular Carcinoma Progression via UFL1‐Mediated Activation of NF‐κB Signaling

Contact Info

Product

Resources

About