LDOC1 Gene Expression in Two Patients with Head and Neck Squamous                     Cell Carcinomas and Parkinson's Disease

Salemi, Michele; Giuffrida, Domenica; Giuffrida, Maria Chiara; Soma, Pier Franco; Rolfo, Alessandro; Cimino, Laura; Condorelli, Rosita A.; Castiglione, Roberto; Vignera, Sandro La; Calogero, Aldo E.

doi:10.1177/030089161209800326

Cited by 4 publications

(5 citation statements)

References 20 publications

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“…However, the same study also revealed that upregulation of LDOC1 in subcases of unmutated chronic lymphatic leukemia was associated with poor prognosis (10). Overexpression of LDOC1 mRNA has also previously been noticed in blood cells of patients with both cutaneous and head and neck squamous cell carcinomas (22,23). Since the same group also noticed elevated LDOC1 mRNA expression in blood samples of Alzheimer patients, the involvement of LDOC1 as a proapoptotic factor in neurodegenerative disease has been assumed (23).…”

Section: Discussionmentioning

confidence: 82%

Loss of LDOC1 Expression by Promoter Methylation in Cervical Cancer Cells

Buchholtz

Jückstöck

Weber

et al. 2013

Cancer Investigation

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Cervical cancer lacks reliable prognostic factors for both progression and chemotherapeutic responsiveness. The expression of the LDOC1 tumor suppressor candidate was therefore investigated. In four of six cervical cancer cell lines tested, expression of LDOC1 was silenced. Downregulation of LDOC1 could also be shown in biopsies of cervical cancer specimens. PCR-based promoter methylation analysis revealed a significant association between promoter methylation and the loss of LDOC1 expression, which could be reverted by DNA methyltransferase inhibitors. This indicates that silencing of LDOC1 is a frequent event in cervical cancer and may be of interest as a molecular marker in cervical cancer.

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Section: Discussionmentioning

confidence: 82%

Loss of LDOC1 Expression by Promoter Methylation in Cervical Cancer Cells

Buchholtz

Jückstöck

Weber

et al. 2013

Cancer Investigation

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show abstract

“…, in a study of expression array on to male young onset hypertension, have suggested that innate immune response and cell‐proliferation regulation may play important downstream roles in development of hypertension and specifically that LDOC1 gene plays a key role in the regulatory mechanisms related to apoptosis in hypertension. In addition, LDOC1 gene expression was increased in Down syndrome subjects and in patients with Parkinson's disease .…”

Section: Discussionmentioning

confidence: 99%

LDOC1 Gene Expression in Men With Klinefelter Syndrome

Salemi

Condorelli

Longo

et al. 2016

Clinical Laboratory Analysis

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Klinefelter syndrome (KS) results from an extra chromosome X, which is due to the failure of normal chromosomal segregation during meiosis. Patients with KS have gynecomastia, small testes, and azoospermia. Apoptosis is a mechanism responsible for the normal regulation of spermatogenesis. LDOC1 gene is a known regulator of nuclear factor mediated pathway to apoptosis through inhibition of nuclear factor kappa B (NF-kappaB). Furthermore, the transcription factor myeloid zinc finger gene 1 (MZF-1) has been shown to interact with LDOC1 and to enhance LDOC1 activity favoring apoptosis. We investigated the expression of LDOC1 gene mRNA, by quantitative reverse transcription polymerase chain reaction (qRT-PCR), in peripheral blood leukocytes of 13 patients with KS compared to 13 healthy men chosen as controls. LDOC1 expression was higher in 9 of the 13 KS patient compared to normal controls. These finding led us to hypothesize that LDOC1 gene upregulation may play a role in the spermatogenesis derangement observed in patients with KS.

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“…RNA extraction, RT and qPCR for each sample were conducted as previously reported (13). LDOC-1 gene expression level was normalized to glyceraldehyde 3-phosphate dehydrogenase gene expression level, and the target mean crossing point definition was used to indicate the mean normalized cycle threshold (13).…”

Section: Methodsmentioning

confidence: 99%

“…The leucine zipper, down-regulated in cancer 1 (LDOC-1) gene, mapping on chromosome Xq27, codes for a nuclear protein of 146 amino acids with a molecular weight of ~17 kDa, and consists of only one exon (Online Mendelian Inheritance in Man 300402) (10). Literature data (10)(11)(12)(13)(14) have indicated greater or lesser expression of LDOC-1 in a variety of tumor types. The aim of the present study was to investigate the expression of the LDOC-1 gene in LNCaP and PC3 PCa cell lines compared with normal prostate cell lines PNT1A and PNT2 by reverse transcription-quantitative polymerase chain reaction (RT-qPCR).…”

Section: Introductionmentioning

confidence: 99%

Leucine zipper, down regulated in cancer-1 gene expression in prostate cancer

et al. 2016

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Abstract. Numerous genetic alterations have been implicated in the development of prostate cancer (PCa). DNA and protein microarrays have enabled the identification of genes associated with apoptosis, which is important in PCa development. Despite the molecular mechanisms are not entirely understood, inhibition of apoptosis is a critical pathophysiological factor that contributes to the onset and progression of PCa. Leucine zipper, down-regulated in cancer 1 (LDOC-1) is a known regulator of the nuclear factor (NF)-mediated pathway of apoptosis through the inhibition of NF-κB. The present study investigated the expression of the LDOC-1 gene in LNCaP, PC-3, PNT1A and PNT2 prostate cell lines by reverse transcription-quantitative polymerase chain reaction. In addition LDOC-1 protein expression in normal prostate tissues and PCa was studied by immunohistochemistry. LDOC-1 messenger RNA resulted overexpressed in LNCaP and PC-3 PCa cell lines compared with the two normal prostate cell lines PNT1A and PNT2. The results of immunohistochemistry demonstrated a positive cytoplasmic LDOC-1 staining in all PCa and normal prostate samples, whereas no nuclear staining was observed in any sample. Furthermore, a more intense signal was evidenced in PCa samples. LDOC-1 gene overexpression in PCa suggests an activity of LDOC-1 in PCa cell lines.

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LDOC1 Gene Expression in Two Patients with Head and Neck Squamous Cell Carcinomas and Parkinson's Disease

Cited by 4 publications

References 20 publications

Loss of LDOC1 Expression by Promoter Methylation in Cervical Cancer Cells

Loss of LDOC1 Expression by Promoter Methylation in Cervical Cancer Cells

LDOC1 Gene Expression in Men With Klinefelter Syndrome

Leucine zipper, down regulated in cancer-1 gene expression in prostate cancer

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