LEA Proteins

Cuming, Andrew C.

doi:10.1007/978-94-011-4431-5_32

Cited by 138 publications

(179 citation statements)

References 66 publications

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“…They are listed in Table IV. The majority of these proteins corresponded to proteins normally synthesized during seed development, such as 12S globulin subunits and members of the dehydrin family (group 2 late embryogenesis abundant [LEA] proteins; Finkelstein, 1993;Bewley and Black, 1994;Cuming, 1999). In other words, it seemed that transcriptional inhibition by a-amanitin somehow allowed the germinating seeds to recapitulate part of the maturation program, by favoring the use of stored mRNAs encoding maturation proteins.…”

Section: De Novo Protein Patternsmentioning

confidence: 99%

The Effect of α-Amanitin on the Arabidopsis Seed Proteome Highlights the Distinct Roles of Stored and Neosynthesized mRNAs during Germination

et al. 2004

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To investigate the role of stored and neosynthesized mRNAs in seed germination, we examined the effect of a-amanitin, a transcriptional inhibitor targeting RNA polymerase II, on the germination of nondormant Arabidopsis seeds. We used transparent testa mutants, of which seed coat is highly permeable, to better ascertain that the drug can reach the embryo during seed imbibition. Even with the most permeable mutant (tt2-1), germination (radicle protrusion) occurred in the absence of transcription, while subsequent seedling growth was blocked. In contrast, germination was abolished in the presence of the translational inhibitor cycloheximide. Taken together, the results highlight the role of stored proteins and mRNAs for germination in Arabidopsis and show that in this species the potential for germination is largely programmed during the seed maturation process. The a-amanitin-resistant germination exhibited characteristic features. First, this germination was strongly slowed down, indicating that de novo transcription normally allows the synthesis of factor(s) activating the germination rate. Second, the sensitivity of germination to gibberellic acid was reduced 15-fold, confirming the role of this phytohormone in germination. Third, de novo synthesis of enzymes involved in reserve mobilization and resumption of metabolic activity was repressed, thus accounting for the failure in seedling establishment. Fourth, germinating seeds can recapitulate at least part of the seed maturation program, being capable of using mRNAs stored during development. Thus, commitment to germination and plant growth requires transcription of genes allowing the imbibed seed to discriminate between mRNAs to be utilized in germination and those to be destroyed.

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Section: De Novo Protein Patternsmentioning

confidence: 99%

The Effect of α-Amanitin on the Arabidopsis Seed Proteome Highlights the Distinct Roles of Stored and Neosynthesized mRNAs during Germination

et al. 2004

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“…LEA1) possibly serve as water-binding domains, act as hydration buffer to regulate water status, or may also interact with the surface of macromolecules as a water matrix to oppose protein denaturation in the dehydrating tissues thereby solvating the cytosolic structures (McCubbin et al, 1985;Baker et al, 1988;Dure, 1993;Cuming, 1999). The charged amino acid motifs in LEA3 may aid in sequestering ions that accumulate under water deficit conditions (Dure, 1993).…”

Section: Lea Proteinsmentioning

confidence: 99%

Genetic approaches towards overcoming water deficit in plants - special emphasis on LEAs

Khurana

Vishnudasan

Chhibbar

2008

Physiol Mol Biol Plants

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Water deficit arises as a result of low temperature, salinity and dehydration, thereby affecting plant growth adversely and making it imperative for plants to surmount such situations by acclimatizing/adapting at various levels. Water deficit stress results in significant changes in gene expression, mediated by interconnected signal transduction pathways that may be triggered by calcium, and regulated via ABA dependent and/or independent pathways. Hence, adaptation of plants to such stresses involves maintaining cellular homeostasis, detoxification of harmful elements and also growth alterations. Stress in general cause excess production of reactive oxygen species (ROS) and the plants overcome the same by either preventing the accumulation of ROS or by eliminating the ROS formed. Ion homeostasis includes processes such as cellular uptake, sequestration and export in conjunction with long distance transport. Requisite amounts of osmolytes are hence synthesized under stress to maintain turgor along with maintaining the macromolecular structures and also for scavenging ROS. Another noteworthy response is the accumulation of novel proteins, including enzymes involved in the biosynthesis of osmoprotectants, heat-shock proteins (HSPs), late embryogenesis abundant (LEA) proteins, antifreeze proteins, chaperones, detoxification enzymes, transcription factors, kinases and phosphatases. The LEAs belong to a redundant protein family and are highly hydrophilic, boiling-soluble, non-globular and therefore have been defined and classified accordingly. The precise function of LEAs is still unknown, but substantial evidence indicates their involvement in dessication tolerance as the expression of LEAs confers increased resistance to stress in heterologous yeast system and also significantly improves water deficit tolerance in transgenic plants. Genetic manipulation of plants towards conferring abiotic stress tolerance is a daunting task, as the abiotic stress tolerance mechanism is highly complex and various strategies have been exploited to address and evaluate the stress tolerance mechanism, and the molecular responses to water deficit via complex signaling networks. Genomic technologies have recently been useful in integrating the multigenicity of the plant stress responses through, transcriptomics, proteomics and metabolite profilling and their interactions. This review deals with the recent developments on genetic approaches for water stress tolerance in plants, with special emphasis on LEAs.

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“…Based on sequence similarity and amino acid representation, LEA proteins have been assigned to various families and groups, but the mechanisms by which cellular protection is conferred under water stress are still poorly understood (Battaglia et al, 2008;Cuming, 1999;Tunnacliffe and Wise, 2007;Wise and Tunnacliffe, 2004). Interaction with phospholipids was reported for COR15am, a group 3 LEA protein, which was shown to interact with membranes during phase transition to the frozen state in Arabidopsis thaliana (Steponkus et al, 1998), and reports suggest that gaining secondary structure during desiccation may contribute to the ability of LEA proteins to stabilize proteins, membranes, and sugar glasses during drying (Furuki et al, 2011;Li and He, 2009;).…”

Section: Introductionmentioning

confidence: 99%

Improved tolerance to salt and water stress in Drosophila melanogaster cells conferred by late embryogenesis abundant protein

Marunde

Samarajeewa

Anderson

et al. 2013

Journal of Insect Physiology

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Graphical AbstractHighlights: ØThe Late Embryogenesis Abundant protein AfLEA1.3 from Artemia franciscana accumulates in the mitochondrion of Drosophila Kc167 cells. Ø AfLEA1.3 improves mitochondrial functions in presence of high sodium chloride concentrations. Ø AfLEA1.3 reduces mitochondrial damage during freeze-thawing. Ø AfLEA1.3 increases cellular tolerance to osmotic stress. Ø AfLEA1.3 increases cellular tolerance to convective drying.

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LEA Proteins

Cited by 138 publications

References 66 publications

The Effect of α-Amanitin on the Arabidopsis Seed Proteome Highlights the Distinct Roles of Stored and Neosynthesized mRNAs during Germination

The Effect of α-Amanitin on the Arabidopsis Seed Proteome Highlights the Distinct Roles of Stored and Neosynthesized mRNAs during Germination

Genetic approaches towards overcoming water deficit in plants - special emphasis on LEAs

Improved tolerance to salt and water stress in Drosophila melanogaster cells conferred by late embryogenesis abundant protein

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