LEA13 and LEA30 Are Involved in Tolerance to Water Stress and Stomata Density in Arabidopsis thaliana

López-Cordova, Abigael; Ramírez-Medina, Humberto; Silva-Martínez, Guillermo A; González-Cruz, Leopoldo; Bernardino-Nicanor, Aurea; Huanca‐Mamani, Wilson; Montero-Tavera, Víctor; Tovar-Aguilar, Andrea; Ramírez-Pimentel, Juan Gabriel; Durán-Figueroa, N. V.; Acosta-García, Gerardo

doi:10.3390/plants10081694

Cited by 6 publications

(4 citation statements)

References 60 publications

(83 reference statements)

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“…Additionally, an increasing number of studies have found that LEA may be involved in more regulatory mechanisms. For example, the mutant of AtLEA13 and AtAtLEA30 were found to be more sensitive to drought stress due to their increased transpiration and increased stomatal density [45]. The overexpression of the OsLEA1a gene in rice could protect plants from various abiotic stresses by preventing cell membrane damage and increasing the plant's ability to scavenge reactive oxygen species [37].…”

Section: Discussionmentioning

confidence: 99%

The Late Embryogenesis Abundant Proteins in Soybean: Identification, Expression Analysis, and the Roles of GmLEA4_19 in Drought Stress

Guo,

Zhang,

Yang

et al. 2023

IJMS

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Late embryogenesis abundant (LEA) proteins play important roles in regulating plant growth and responses to various abiotic stresses. In this research, a genome-wide survey was conducted to recognize the LEA genes in Glycine max. A total of 74 GmLEA was identified and classified into nine subfamilies based on their conserved domains and the phylogenetic analysis. Subcellular localization, the duplication of genes, gene structure, the conserved motif, and the prediction of cis-regulatory elements and tissue expression pattern were then conducted to characterize GmLEAs. The expression profile analysis indicated that the expression of several GmLEAs was a response to drought and salt stress. The co-expression-based gene network analysis suggested that soybean LEA proteins may exert regulatory effects through the metabolic pathways. We further explored GnLEA4_19 function in Arabidopsis and the results suggests that overexpressed GmLEA4_19 in Arabidopsis increased plant height under mild or serious drought stress. Moreover, the overexpressed GmLEA4_19 soybean also showed a drought tolerance phenotype. These results indicated that GmLEA4_19 plays an important role in the tolerance to drought and will contribute to the development of the soybean transgenic with enhanced drought tolerance and better yield. Taken together, this study provided insight for better understanding the biological roles of LEA genes in soybean.

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Section: Discussionmentioning

confidence: 99%

The Late Embryogenesis Abundant Proteins in Soybean: Identification, Expression Analysis, and the Roles of GmLEA4_19 in Drought Stress

Guo,

Zhang,

Yang

et al. 2023

IJMS

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“…Furthermore, another factor influencing stomatal density is the level of certain protein groups in the late stages of embryogenesis (LEA). López-Cordova et al (2021) explained that LEA (Late Embryogenesis Abundant) protein is involved in the tolerance mechanism against drought stress and stomatal density. The result of this study found that both cowpea and long bean plants had a similar response by reducing the number of stomata (stomatal density) and the width of the stomatal opening under drought stress.…”

Section: Discussionmentioning

confidence: 99%

Comparative study of morphophysiological responses among cowpea and long beans plants under drought stress condition

SYAFRIANI

SUSILO²,

SAYEKTI³

et al. 2022

Biodiversitas

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Abstract. Syafriani E, Susilo KR, Sayekti RS, Widyawan MH, Khoirunnisa NS, Rahmadhani M. 2022. Comparative study of morphophysiological responses among cowpea and long beans plants under drought stress condition. Biodiversitas 23: 5507-5518. Long bean plants are generally more susceptible to drought stress than cowpea plants. One of the promising solutions is through plant breeding to produce genetically modified long bean plants resistant to drought stress by crossing with cowpeas. Parental selection is the initial stage of the plant breeding program. This stage was performed by selecting parental varieties based on the desired superior characteristics, including morphological and physiological characteristics, as well as, yield production. Unfortunately, the comparison between long bean plants and cowpea plants is not well-studied. Therefore, the aim of this study was to analyze the morphophysiological similarities and differences between long beans and cowpeas to withstand drought stress. A two-factorial randomized block design was used in this study and consisted of 2 factors (plant varieties and type of drought stress). The plant varieties used in this study are 7 long bean plants and 7 cowpea plants. While the level of drought stress were: T0 (watering every day), T1 (every 5 days), and T2 (every 10 days). The observational data from morphophysiological characters indicated that long bean plants were more tolerant to drought stress than cowpea plants. Interestingly, one of the cowpea varieties (C2) had the highest proline contents (20.27 µmol/gram of leaves sample) compared to other varieties. Hence, the C2 variety can be considered a candidate for further studies. Meanwhile, all the tested 7 varieties of long bean plants showed varying adaptability, possibly due to the genetic diversity of each variety against drought stress.

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“…Late embryogenesis abundant (LEA) proteins are a large protein family that mainly functions in protecting cells from abiotic stress; however, these proteins are also involved in drought tolerance and the determination of stomatal patterning and density. The overexpression of LEA13 and LEA30 , CaLEA6 and CaLEA73 (from Capsicum annuum ), and MsLEA4-4 (from Medicago sativa ) genes in Arabidopsis thaliana plants increased drought tolerance due to lower transpiration and stomatal density compared to control plants [ 82 ]. Homeodomain leucine zipper (HD-Zip) transcription factors are plant-specific TFs that participate in abiotic stress responses, and MdHB7-like (an HD-Zip I TF) was identified as a drought-induced gene in apple plants.…”

Section: Molecular Regulation Of Stomatal Density For Osmotic Stress ...mentioning

confidence: 99%

How Does Stomatal Density and Residual Transpiration Contribute to Osmotic Stress Tolerance?

Hasanuzzaman

Zhou

Shabala

2023

Plants

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Osmotic stress that is induced by salinity and drought affects plant growth and development, resulting in significant losses to global crop production. Consequently, there is a strong need to develop stress-tolerant crops with a higher water use efficiency through breeding programs. Water use efficiency could be improved by decreasing stomatal transpiration without causing a reduction in CO2 uptake under osmotic stress conditions. The genetic manipulation of stomatal density could be one of the most promising strategies for breeders to achieve this goal. On the other hand, a substantial amount of water loss occurs across the cuticle without any contribution to carbon gain when the stomata are closed and under osmotic stress. The minimization of cuticular (otherwise known as residual) transpiration also determines the fitness and survival capacity of the plant under the conditions of a water deficit. The deposition of cuticular wax on the leaf epidermis acts as a limiting barrier for residual transpiration. However, the causal relationship between the frequency of stomatal density and plant osmotic stress tolerance and the link between residual transpiration and cuticular wax is not always straightforward, with controversial reports available in the literature. In this review, we focus on these controversies and explore the potential physiological and molecular aspects of controlling stomatal and residual transpiration water loss for improving water use efficiency under osmotic stress conditions via a comparative analysis of the performance of domesticated crops and their wild relatives.

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LEA13 and LEA30 Are Involved in Tolerance to Water Stress and Stomata Density in Arabidopsis thaliana

Cited by 6 publications

References 60 publications

The Late Embryogenesis Abundant Proteins in Soybean: Identification, Expression Analysis, and the Roles of GmLEA4_19 in Drought Stress

The Late Embryogenesis Abundant Proteins in Soybean: Identification, Expression Analysis, and the Roles of GmLEA4_19 in Drought Stress

Comparative study of morphophysiological responses among cowpea and long beans plants under drought stress condition

How Does Stomatal Density and Residual Transpiration Contribute to Osmotic Stress Tolerance?

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