Peat is commonly used as the main component of horticultural substrates, but it has a very low buffering capacity for the anionic macronutrient phosphorus (P), which can be increased by the addition of clays. The aim of this study was to characterize the P adsorption capacity of different substrate clays and to evaluate its significance for plant P uptake. Substrate clays were characterized with a single-point batch experiment and adsorption and desorption isotherms. The data were fitted to the Langmuir equation for a calculation of the maximum adsorption capacity. Additionally, the contents of oxalate extractable Fe and Al (SFe ox + Al ox ) were determined. The influence of a varying P adsorption capacity of the clays on the P availability to plants in the respective peat-clay substrates and pure peat was investigated in a growth experiment with Impatiens walleriana fertigated with 0, 17, and 35 mg P L -1 solution, respectively. The observed and calculated (Langmuir) P adsorption capacity of the clays could be well-characterized by both the batch experiment and the adsorption isotherms and was highly correlated with the SFe ox +Al ox . A higher P adsorption capacity of the clay amendment in mixed substrates resulted in a lower P concentration in the substrate solution, while the CAT extractable P concentration (P CAT ) was the same. Plant growth and shoot P concentrations were enhanced in the substrates, showing a higher P adsorption capacity, since plants were able to take up the whole amount of P CAT , and also part of the non-CAT extractable P. However, the release rate was too low to ensure optimal plant growth, which was in accordance with the result of the desorption experiment. The absolute extent of P release was increased with the increasing P adsorption capacity of the clays and higher degree of P saturation (DPS).