Lead-binding proteins - a way to understand lead toxicity?

Bergdahl, Ingvar A.

doi:10.1051/analusis:199826060081

Cited by 14 publications

(3 citation statements)

References 12 publications

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“…Due to the numerous molecular and biochemical effects of lead in adults, children, and infants, understanding the mechanisms of lead toxicity still remains as a major challenge to researchers ( Ellenhorn 1997 ; ATSDR 1998 ; Bergdahl 1998 ). Lead exposure has been associated with increased risk of lung, stomach, and bladder cancer in diverse human populations ( Fu and Boffetta 1995 ; Steenland and Boffetta 2000 ; NTP 2003 ).…”

Section: Introductionmentioning

confidence: 99%

Polymorphisms in MGP gene and their association with lead toxicity

Shaik

Jamil²

2009

Toxicology Mechanisms and Methods

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Matrix γ-carboxy glutamic acid protein (MGP) is a 10-kDa secreted protein containing five residues of the vitamin K-dependent calcium binding amino acid γ-carboxyglutamic acid (Gla). This study was carried out to examine the effects of MGP gene promoter polymorphism (T-138C) on blood lead levels (BLL) and hematological parameters in 113 battery manufacturing unit workers occupationally exposed to lead and 102 controls. Genotypes for the MGP T-138C polymorphism were determined by PCR and restriction fragment length digestion. BLL were determined by Anode Stripping Voltammetry using ESA Model 3010B Lead analyzer. Complete blood picture (CBP) was analyzed using ADVIA Cell counter for each sample. The frequencies of MGP–TT, CT and CC genotypes in our population were 38.6%, 44.3%, and 17.2%, respectively. The frequencies for T and C alleles were 0.612 and 0.386, respectively. Although BLL did not differ significantly among genotypes; they were higher in workers with TT/CT genotype compared to CC genotype subjects (76–88 μg/dL vs 22–45 μg/dL, p > 0.05). About 29.2% of volunteers (n = 33) from the occupationally exposed group had hemoglobin levels below 10.0 gms/dl. There was no significant difference in total white cell count and platelet count between occupational and non-exposed groups. The possible role of SNPs in the promoter region of MGP gene with relation to lead toxicity was investigated for the first time in the Indian population; although significance could not be achieved in this study, further assessments over a larger population size may help in better understanding of the consequences of lead exposure.

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Section: Introductionmentioning

confidence: 99%

Polymorphisms in MGP gene and their association with lead toxicity

Shaik

Jamil²

2009

Toxicology Mechanisms and Methods

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“…Earlier, we have showed lead-induced genotoxicity in vitro using cultured human peripheral blood lymphocytes (Shaik, et al, 2006). δ-aminolevulinic acid dehydratase (ALAD) has been widely acknowledged to play an important role in the pathogenesis of lead poisoning (Bergdahl, 1998;Astrin, et al, 1987).…”

Section: Introductionmentioning

confidence: 99%

A study on the ALAD gene polymorphisms associated with lead exposure

Shaik¹,

Jamil²

2008

Toxicol Ind Health

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Delta -aminolevulinic acid dehydratase (ALAD) plays an important role in lead poisoning, and polymorphisms in this gene may affect the response of individuals to lead toxicity symptoms. This study was carried out to examine the effects of ALAD gene polymorphism (G177C) on blood lead levels (BLL) and hematological parameters. In all, 113 battery manufacturing unit workers and 102 occupationally unexposed controls from Hyderabad, A.P, India formed the study group. Genotypes for the ALAD G177C polymorphism were determined by Polymerase chain reaction and restriction fragment length digestion. BLL were determined by anode stripping voltammetry using ESA Model 3010B Lead analyzer. Complete blood picture was analyzed using ADVIA cell counter for each sample. The frequencies for ALAD1 and ALAD2 alleles were 0.98 and 0.01, respectively. ALAD 1-2 and ALAD 2-2 genotypes together were considered as a single group and compared with the ALAD 1-1 genotype group. BLL did not differ significantly among ALAD1-1, 1-2, and 2-2 genotypes; however, subjects from the ALAD 1-2/2-2 genotype group showed higher BLL concentrations of 80.51 microg/dL when compared with subjects from the ALAD 1-1 genotype group (50.4 microg/dL). Approximately 29.2% volunteers (nA =A 33) from the occupationally exposed group had hemoglobin levels below 10.0 g/dL. There was no significant difference in total white cell count and platelet count between occupational and non-exposed lead-exposed groups. BLL of occupationally exposed individuals were significantly high compared with the unexposed group. ALAD G177C polymorphism along with BLL and assessment of hematological parameters may play an important role in evaluation and better understanding of the consequences of lead exposure.

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“…Указанный металл занимает второе место в двадцатке самых патогенных элементов (после мышьяка). Его органами-мишенями являются кости, кровь, почки, репродуктивная и сердечно-сосудистая системы, а также мозг и щитовидная железа [Bergdahl, 1988;HMRC, 2003].…”

Section: Introductionunclassified

Экологическое Состояние Водных Ресурсов Рек Ирака

Иванович¹,

Али²,

Александровна³

et al. 2021

РГ

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Аннотация. Основными направлениями использования вод в Ираке являются орошение, выработка электроэнергии и питьевое водоснабжение. При этом наиболее активным потребителем воды является сельское хозяйство. В настоящей работе рассмотрена проблема загрязнения природной пресной воды рек Ирака катионами тяжелых металлов, поскольку к настоящему времени данный аспект недостаточно изучен. Целью исследования является комплексный сравнительный анализ уровня содержания тяжелых металлов в водах реки Евфрат. Показано, что бесконтрольный сброс в речные бассейны рек Тигр и Евфрат сточных вод, имеющих высокое содержание поллютантов бытового и техногенного происхождения, привело к прогрессирующей неудовлетворительной экологической ситуации. Приведены результаты разовых экспериментальных исследований проб воды реки Евфрат из 8 станций водозабора. Выявлено превышение в природных водах предельно допустимой концентрации катионов таких тяжелых металлов, как никель, кадмий и свинец более чем в 200 раз. Сделан вывод о том, что Загрязнение природной воды рек Ирака является следствием не только климатических особенностей, но и отсутствия должного надзора за предприятиями, осуществляющими бесконтрольный сброс сточных вод в природную гидросферу и неудовлетворительным состоянием городских очистных сооружений.

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Lead-binding proteins - a way to understand lead toxicity?

Cited by 14 publications

References 12 publications

Polymorphisms in MGP gene and their association with lead toxicity

Polymorphisms in MGP gene and their association with lead toxicity

A study on the ALAD gene polymorphisms associated with lead exposure

Экологическое Состояние Водных Ресурсов Рек Ирака

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