The environmental implications involved in battery plant are increasing concern due to the elevated metal concentrations in the surrounding soils. Earthworms are regarded as a model organism for evaluation of terrestrial environmental pollution. The biochemical responses in the earthworms, Eisenia fetida (E. fetida) exposed to polluted soils were investigated. Soil samples at 0, 50, 100, 200, 500 and 1000 m from battery plant were collected and used to assess the relationship between sampling distance and biochemical responses in the E. fetida.Biochemical alterations were carried out on 1, 3, 7, and 14 d of exposure. The metallothionein (MT), malondialdehyde (MDA) and antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD)) were measured. The results demonstrated that the concentrations of heavy metals, especially for Pb, decreased sharply with increase in distance, which may be due to the leaching and accumulation of Pb in soil during production processes. The concentrations of MT and MDA increased with exposure dosage and time, indicating that E. fetida is presented dose-and time dependence after heavy metal exposure. The activities of SOD and CAT increased after heavy metal induced, while the activity of POD was lower than that of control group. The SOD, CAT and POD present a co-operative effect for the protection of lipids Downloaded by [University of Nebraska, Lincoln] at 01:52 08 June 2016 ACCEPTED MANUSCRIPT ACCEPTED MANUSCRIPT 2 and enzymes due to the pollution. The soils around battery plant were polluted with metals and has adverse biological effects.