Lead exposure-induced changes in hematology and biomarkers of hepatic injury: protective role of TrévoTM supplement

Abstract

Lead exposure has been linked to health challenges involving multiple organ failure. More than fifty percent of lead present in the human body is accumulated in the liver causing hepatic injury. A major mechanism of lead toxicity is oxidative stress. TrévoTM is a nutritional supplement with numerous bioactive natural products with detoxifying and antioxidant properties. This study was designed to investigate the hepatoprotective effects of TrévoTM dietary supplements against lead-hepatotoxicity in male Wistar rats. Thirty-five healthy animals were divided into five groups of seven each as follows: Group I=control; II=15 mg/kg of lead acetate (PbA); III= 2 mL/kg of TrévoTM + PbA; IV= 5 mL/kg of TrévoTM + PbA;V=5 mL/kg of TrévoTM . Animals were orally treated with TrévoTM for two days before co-administration with PbA intraperitoneally for 12 consecutive days. Animals were sacrificed 24 h after the last administration and blood were collected via cardiac puncture and processed for hematological parameters and assessment of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and albumin (ALB). The liver was excised and processed for markers of oxidative stress and histopathological examination. Intraperitoneal administration of 15 mg/kg of PbA caused a significant increase in serum concentration of AST, ALT, while the concentration of ALB was significantly decreased (P<0.001). PbA caused a significant reduction in packed cell volume, hemoglobin while the total white blood cell count, neutrophils, lymphocytes, monocytes, eosinophils, and basophils were increased. Oxidative stress was significantly pronounced in the liver of rats exposed to PbA as observed in the high concentration of malonedialdehyde, decreased concentration of glutathione, the activity of catalase, superoxide dismutase, and glutathione-S-transferase. Pretreatment with TrévoTM was able to significantly prevent the anemic, oxidative damage, and hepatic injury initiated by PbA. Histological examination also corroborated the biochemical results. In conclusion, the study reveals that TrévoTM is effective in attenuating PbA-induced hepatotoxicity in male Wistar rats.

Antagonistic effectiveness of Anacardium occidentale leaf extract on lead-acetate exposure-induced hepatorenal toxicity in rats

“…The increase in these markers was caused by both the rupture of the glomerular filtration barrier and alterations in hepatocyte membrane permeability, which allowed these enzymes to seep into the circulation [ 45 , 46 ]. High levels of key markers for liver and renal dysfunction have been used to support the hypothesis that Pb causes hepatorenal toxicity [ 47 , 48 ]. Free radicals produced by PbAc, which increase levels of lipid peroxidation and compete with calcium, a crucial component for maintaining the integrity of cell membranes, may be responsible for the disruption of the hepatic membrane's permeability and the glomerular filtration membrane [ 45 ].…”

Microplastics exposure altered hematological and lipid profiles as well as liver and kidney function parameters in albino rats (Rattus norvegicus)

“…Additionally, a 10 % lead acetate solution was prepared to serve as positive control. Lead acetate is a known hematobiochemical toxicant as demonstrated by Ilesanmi et al [ 28 ].…”

Synergistic ameliorative effect of ascorbic acid and Moringa oleifera in lead-induced toxicity: a review

“…Different bioactive substances that are obtained from plants have huge therapeutic and minimal side effects, making them important raw materials for pharmaceutical industries (Calixto, 2019). The importance of natural substances in arresting heavy metal toxicity is on the rise (Ilesanmi et al, 2022). Antioxidants can be used as good antidotes to Pb toxicity since the major mechanism of Pb toxicity is oxidative stress.…”