Lead-Induced Hepato-Renal Toxicosis and the Ameliorative Role of Rhodiolarosea Extract in Albino Rats

Mahamat, Ibrahim A.; Mehanna, El Sayed E.; Oda, Samah S.; Khafaga, Asmaa F.; El-Karim, Dina R. S. Gad

doi:10.5455/ajvs.90234

Cited by 3 publications

(10 citation statements)

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“…Muhammed et al (3) and Ibrahim et al(20) in their studies reported similar results for the liver tissue structure of the control group. Our results also showed, in lead-exposed treatments, several changes such as kupffer cell necrosis, mild to severe dilation of sinusoids, and the mild influx of inflammatory cells was observed.…”

supporting

confidence: 57%

“…Our results for ALT, AST and ALP were in line with their findings. This significance effects may be due to the accumulation of lead in liver as one of the major sites for the excretion and presence of lead-binding proteins in this organ (20). The results of Ramesh et al…”

Section: Biochemical Studiesmentioning

confidence: 97%

“…In the present study, the liver of the control and sham groups had a normal structure with hepatocyte cords, central vein, sinusoids, Kupffer cells, and distinct portal area. (20). Liver histological study of leadtreated animals by Kubo et al (22) showed damage to the liver structure, necrosis, and inflammatory cells infiltration.…”

Section: Histopathologymentioning

confidence: 98%

“…Changes such as hydropic degeneration, cell vacuolation, and cellular necrosis due to lead exposure were observed in this study which according to Jarrar & Taib (23) may be due to increased cellular activity and loss of nucleus during the detoxification mechanism. Ibrahim et al (20) stated that induced changes in liver structure of lead administrated rats to the disruption of the body's antioxidant balance.…”

Section: Histopathologymentioning

confidence: 99%

“…Muhammed et al(3) stated that, in the leadexposed group, some defects such as necrosis and apoptosis of Kupffer cells, the influx of inflammatory cells and dilation of sinusoids were observed. Also, Ibrahim et al(20) reported that exposure to lead acetate induce several negative change in liver histologic structure such as: marked midzonal and periportal vacuolar degeneration, focal areas of coagulative necrosis represented by nuclear pyknosis, infiltration of mononuclear inflammatory cells, distention in the portal area, congestion of portal vein, perivascular edema and mononuclear cell infiltration, and hepatocyte vacuolization. In the other similar study, Abdelhamid et al(21) reported that exposure to lead acetate induced many pathologic changes such as hydropic degeneration, hepatocytes necrosis, bile duct hyperplasia, and portal vein and central vein congestion in liver structure.…”

mentioning

confidence: 99%

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Prenatal, lactation and postnatal effects of lead acetate on histological, histochemical and hepatic indices of liver of male offspring wistar rats

Roomiani

Morovvati

Asaadi

et al. 2022

ijhs

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The aim of this study was to investigate the possible effects of maternal administration to lead acetate on the morphological structure of the liver; AST, ALT, ALP and GGT levels and glycogen content of hepatocytes of male offspring wistar rats. Female rats were exposed to 0.2% lead acetate in drinking water and the study was performed on their male offspring (7 groups). To evaluate the levels of liver enzymes, blood samples were taken from rats after anesthesia. After dissection, to study its morphological structure and glycogen content of hepatocytes, liver tissue was isolated and transferred to 10% formalin. To measure the levels of hepatic enzymes; AST, ALT, ALP and GGT; serum samples were evaluated. For histological examinations, liver tissue samples were embedded in paraffin after tissue processing and 3 μm thickness sections were prepared from them and then H&E and PAS staining was performed. The results showed that exposure to 0.2% lead acetate induced significant changes in liver enzyme levels in different experimental groups.

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supporting

confidence: 57%

Section: Biochemical Studiesmentioning

confidence: 97%

Section: Histopathologymentioning

confidence: 98%

Section: Histopathologymentioning

confidence: 99%

mentioning

confidence: 99%

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Prenatal, lactation and postnatal effects of lead acetate on histological, histochemical and hepatic indices of liver of male offspring wistar rats

Roomiani

Morovvati

Asaadi

et al. 2022

ijhs

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Screening impacts of Tilmicosin induced-hepatic and renal toxicity in rats: Protection by Rhodiola Rosea extract through the involvement of oxidative stress, antioxidants, and inflammatory cytokines biomarkers

Elgendy,

Soliman,

Shukry

et al. 2024

Preprint

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Tilmicosin (TIL) is a semisynthetic macrolide antibiotic with a broad spectrum of activity derived from tylosin. TIL is effective in the treatment of bovine and ovine respiratory diseases caused by different microbes. In parallel, Rhodiola rosea (RHO) is a popular herbal remedy because of its anti-inflammatory and antioxidant qualities. Rats received saline or RHO for sequential 12 days. TIL was injected as a single dose subcutaneously (75 mg/kg BW) on day 6 of experiment. The protective group received RHO daily for sequential 12 days, TIL was injected as a single dose 1 hour after RHO administration on day 6 of experiment and continued for extra 6 successive days with RHO only. Samples and blood were collected for serum analysis, gene expression and immunohistochemistry screening at liver and kidney levels. TIL injection increased serum levels of hepatic and renal markers (ALP, ALT, AST, TC, TG, creatinine and urea) with a decrease in total proteins. In parallel, TIL induced hepatic and renal oxidative stress as there was an increase in malondialdehyde levels, with a decrease in catalase and reduced glutathione activities. Of interest, pre-administration of RHO inhibited TIL-induced increase in hepato-renal markers and decrease the oxidative stress and increased antioxidant activities of both liver and kidney. Quantitative RT-PCR showed that TIL increased HSP70 (heat shock protein), NFkB and TNF-α mRNA expression in liver. Moreover, TIL upregulated the expression of desmin, nestin, and vimentin expression in kidney. The upregulated genes were decreased significantly in the protective group received RHO. Serum inflammatory cytokines, together with genes of inflammatory markers in liver tissues (HSP70, NFkB and TNF-α) and in kidney tissues (desmin, nestin, and vimentin) were all affected. TIL induced hepatic vacuolation and congestion together with glomerular atrophy. The immunoreactivity of PCNA and HMGB1 were examined immunohistochemically. At cellular levels, PCNA was decreased while HMGB1 immunoreactivity was increased in TIL injected rats and was improved by pre administration of RHO. RHO administration protected the altered changes in liver and renal histology. Current findings support the possible use of RHO to shield the liver and kidney from the negative effects of tilmicosin.

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Exploration of Tilmicosin Cardiotoxicity in Rats and the Protecting Role of the Rhodiola rosea Extract: Potential Roles of Cytokines, Antioxidant, Apoptotic, and Anti-Fibrotic Pathways

Elgendy,

Soliman,

Ghamry

et al. 2023

Toxics

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Tilmicosin (TIL) is a common macrolide antibiotic in veterinary medicine. High doses of TIL can have adverse cardiovascular effects. This study examined the effects of Rhodiola rosea (RHO) that have anti-inflammatory, antioxidant, and anti-fibrotic effects on tilmicosin (TIL)-induced cardiac injury targeting anti-inflammatory, antioxidant, apoptotic, and anti-apoptotic signaling pathways with anti-fibrotic outcomes. Thirty-six male Wistar albino rats were randomly divided into groups of six rats each. Rats received saline as a negative control, CARV 1 mL orally (10 mg/kg BW), and RHO 1 mL orally at 400 mg/kg BW daily for 12 consecutive days. The TIL group once received a single subcutaneous injection (SC) dose of TIL (75 mg/kg BW) on the sixth day of the experiment to induce cardiac damage. The standard group (CARV + TIL) received CARV daily for 12 consecutive days with a single TIL SC injection 1 h after CARV administration only on the sixth day of study and continued for another six successive days on CARV. The protective group (RHO + TIL) received RHO daily for the same period as in CARV + TIL-treated rats and with the dosage mentioned before. Serum was extracted at the time of the rat’s scarification at 13 days of study and examined for biochemical assessments in serum lactate dehydrogenase (LDH), cardiac troponin I (cTI), and creatine phosphokinase (CK-MB). Protein carbonyl (PC) contents, malondialdehyde (MDA), and total antioxidant capacity (TAC) in cardiac homogenate were used to measure these oxidative stress markers. Quantitative RT-PCR was used to express interferon-gamma (INF-γ), cyclooxygenase-2 (COX-2), OGG1, BAX, caspase-3, B-cell lymphoma-2 (Bcl-2), and superoxide dismutase (SOD) genes in cardiac tissues, which are correlated with inflammation, antioxidants, and apoptosis. Alpha-smooth muscle actin (α-SMA), calmodulin (CaMKII), and other genes associated with Ca2+ hemostasis and fibrosis were examined using IHC analysis in cardiac cells (myocardium). TIL administration significantly increased the examined cardiac markers, LDH, cTI, and CK-MB. TIL administration also increased ROS, PC, and MDA while decreasing antioxidant activities (TAC and SOD mRNA) in cardiac tissues. Serum inflammatory cytokines and genes of inflammatory markers, DNA damage (INF-γ, COX-2), and apoptotic genes (caspase-3 and BAX) were upregulated with downregulation of the anti-apoptotic gene Bcl-2 as well as the DNA repair OGG1 in cardiac tissues. Furthermore, CaMKII and α-SMA genes were upregulated at cellular levels using cardiac tissue IHC analysis. On the contrary, pretreatment with RHO and CARV alone significantly decreased the cardiac injury markers induced by TIL, inflammatory and anti-inflammatory cytokines, and tissue oxidative-antioxidant parameters. INF-γ, COX-2, OGG1, BAX, and caspase-3 mRNA were downregulated, as observed by real-time PCR, while SOD and Bcl-2 mRNA were upregulated. Furthermore, the CaMKII and α-SMA genes’ immune reactivities were significantly decreased in the RHO-pretreated rats.

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Lead-Induced Hepato-Renal Toxicosis and the Ameliorative Role of Rhodiolarosea Extract in Albino Rats

Cited by 3 publications

References 0 publications

Prenatal, lactation and postnatal effects of lead acetate on histological, histochemical and hepatic indices of liver of male offspring wistar rats

Prenatal, lactation and postnatal effects of lead acetate on histological, histochemical and hepatic indices of liver of male offspring wistar rats

Screening impacts of Tilmicosin induced-hepatic and renal toxicity in rats: Protection by Rhodiola Rosea extract through the involvement of oxidative stress, antioxidants, and inflammatory cytokines biomarkers

Exploration of Tilmicosin Cardiotoxicity in Rats and the Protecting Role of the Rhodiola rosea Extract: Potential Roles of Cytokines, Antioxidant, Apoptotic, and Anti-Fibrotic Pathways

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