Lead induces oxidative stress and phenotypic markers of apoptosis in Saccharomyces cerevisiae

Bussche, J. V.; Soares, Eduardo V.

doi:10.1007/s00253-010-3056-7

Cited by 37 publications

(20 citation statements)

References 35 publications

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“…4). The exposure of yeast cells to Pb for 6 h induced a loss of proliferation capacity with a small loss of membrane integrity, similarly to the one described in S. cerevisiae cells (Bussche and Soares 2011).…”

Section: Discussionmentioning

confidence: 75%

“…Pb is classified as a priority pollutant by the US Environmental Protection Agency (US-EPA 2006) and can cause, in humans, blood and brain disorders (ATSDR 2007). In the yeast Saccharomyces cerevisiae, Pb inhibits assimilation of nutrients (Chen and Wang 2007) and metabolic activity (Van der Heggen et al 2010), decreases the intracellular level of reduced glutathione (Perez et al 2013) and induces oxidative stress (Bussche and Soares 2011;, DNA damage (Cui and Tang 2000;Yu et al 2009;Yuan and Tang 1999), loss of proliferation capacity (Soares et al 2003;Suh et al 1999) and cell death displaying phenotypic markers of apoptosis (Bussche and Soares 2011). Pb is compartmentalized in the vacuole by two parallel pathways: one dependent of H + gradient generated by the V-ATPAse and another mediated by ABCC transporters (Ycf1p, Vmr1p, Ybt1p and Bpt1p), after conjugation with glutathione, in a process catalysed by glutathione transferases 1 and 2 (Gtt1p and Gtt2p) (Sousa et al 2015).…”

Section: Introductionmentioning

confidence: 99%

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Impact of multi-metals (Cd, Pb and Zn) exposure on the physiology of the yeast Pichia kudriavzevii

Mesquita

Machado

Silva

et al. 2015

Environ Sci Pollut Res

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Metal contamination of the environment is frequently associated to the presence of two or more metals. This work aimed to study the impact of a mixture of metals (Cd, Pb and Zn) on the physiology of the non-conventional yeast Pichia kudriavzevii. The incubation of yeast cells with 5 mg/ l Cd, 10 mg/l Pb and 5 mg/l Zn, for 6 h, induced a loss of metabolic activity (assessed by FUN-1 staining) and proliferation capacity (evaluated by a clonogenic assay), with a small loss of membrane integrity (measured by trypan blue exclusion assay). The staining of yeast cells with calcofluor white revealed that no modification of chitin deposition pattern occurred during the exposure to metal mixture. Extending for 24 h, the exposure of yeast cells to metal mixture provoked a loss of membrane integrity, which was accompanied by the leakage of intracellular components. A marked loss of the metabolic activity and the loss of proliferation capacity were also observed. The analysis of the impact of a single metal has shown that, under the conditions studied, Pb was the metal responsible for the toxic effect observed in the metal mixture. Intracellular accumulation of Pb seems to be correlated with the metals' toxic effects observed.

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Section: Discussionmentioning

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Impact of multi-metals (Cd, Pb and Zn) exposure on the physiology of the yeast Pichia kudriavzevii

Mesquita

Machado

Silva

et al. 2015

Environ Sci Pollut Res

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“…The exposition of S. cerevisiae cells to Pb resulted in a marked intracellular ROS accumulation [3]. GSH is the main redox buffer of the cells being a key factor against the oxidative stress and metals toxicity [25].…”

Section: Pb-induced Loss Of Viability In Wild-type and Glutathione-dementioning

confidence: 99%

“…In addition, it was shown that Pb induces the intracellular accumulation of reactive oxygen species (ROS), which can be the trigger of programmed cell death by apoptosis [3].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Role of Glutathione in the Lead-Induced Toxicity in Saccharomyces cerevisiae

et al. 2013

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The effect of intracellular reduced glutathione (GSH) in the lead stress response of Saccharomyces cere-visiae was investigated. Yeast cells exposed to Pb, for 3 h, lost the cell proliferation capacity (viability) and decreased intracellular GSH level. The Pb-induced loss of cell viability was compared among yeast cells deficient in GSH1 (Dgsh1) or GSH2 (Dgsh2) genes and wild-type (WT) cells. When exposed to Pb, Dgsh1 and Dgsh2 cells did not display an increased loss of viability, compared with WT cells. However, the depletion of cellular thiols, including GSH, by treatment of WT cells with iodoacetamide (an alkylating agent, which binds covalently to thiol group), increased the loss of viability in Pb-treated cells. In contrast, GSH enrichment, due to the incubation of WT cells with amino acids mixture constituting GSH (L-glutamic acid, L-cysteine and glycine), reduced the Pb-induced loss of proliferation capacity. The obtained results suggest that intracellular GSH is involved in the defence against the Pbinduced toxicity; however, at physiological concentration, GSH seems not to be sufficient to prevent the Pb-induced loss of cell viability.

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“…Once inside the cells, heavy metals can display several toxicity mechanisms which include oxidative stress, DNA oxidation and enzyme inhibition [3]. The exposure of yeast cells of Saccharomyces cerevisiae to Pb induced a loss of cell proliferation capacity [4,5], nuclear morphological alterations [6] and nuclear DNA damage [7][8][9]. In addition, Pb decreases the intracellular level of reduced glutathione [10] and induces the production of reactive oxygen species, which can be the trigger of programmed cell death by apoptosis [6].…”

Section: Introductionmentioning

confidence: 99%

ABCC Subfamily Vacuolar Transporters are Involved in Pb (Lead) Detoxification in Saccharomyces cerevisiae

Sousa

Hanselaer

Soares

2014

Appl Biochem Biotechnol

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The present work has as objective to contribute for the elucidation of the mechanism associated with Pb detoxification, using the yeast Saccharomyces cerevisiae as a model organism. The deletion of GTT1 or GTT2 genes, coding for functional glutathione transferases (GST) enzymes in S. cerevisiae, caused an increased susceptibility to high Pb concentrations (500-1000 μmol L−1). These results suggest that the formation of glutathione-Pb conjugate (GS-Pb), dependent of GSTs, is important in Pb detoxification. The involvement of ATPbinding cassette (ABC) vacuolar transporters, belonging to class C subfamily (ABCC) in vacuolar compartmentalization of Pb, was evaluated. For this purpose, mutant strains disrupted in YCF1, VMR1, YBT1 or BPT1 genes were used. All mutants tested, without vacuolar ABCC transporters, presented an increased sensitivity to 500-1000 μmol L−1 Pb comparative to wildtype strain. Taken together, the obtained results suggest that Pb detoxification, by vacuolar compartmentalization, can occur as a result of the concerted action of GSTs and vacuolar ABCC transporters. Pb is conjugated with glutathione, catalysed by glutathione transferases and followed to the transport of GS-Pb conjugate to the vacuole by ABCC transporters.

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Lead induces oxidative stress and phenotypic markers of apoptosis in Saccharomyces cerevisiae

Cited by 37 publications

References 35 publications

Impact of multi-metals (Cd, Pb and Zn) exposure on the physiology of the yeast Pichia kudriavzevii

Impact of multi-metals (Cd, Pb and Zn) exposure on the physiology of the yeast Pichia kudriavzevii

Evaluation of the Role of Glutathione in the Lead-Induced Toxicity in Saccharomyces cerevisiae

ABCC Subfamily Vacuolar Transporters are Involved in Pb (Lead) Detoxification in Saccharomyces cerevisiae

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