Leaf anatomy micromorphometry plasticity and histochemistry of Azadirachta indica during acclimatization

Abstract

Environmental conditions of grow can modify leaf structure and metabolite production. Neem plants produce a high amount of medicinal metabolites and contain biopesticide terpenoids with low toxicity. However, the high genetic variation and the low material quality, besides the environmental modifications warn to the need of biotechnological techniques to ensure the production of high quality metabolites. The aim was to investigate leaf structural and histochemical characteristics of Azadirachta indica grown in vitro, in vivo and acclimatized condition. It was found anatomical differences among the environments, with higher leaf thickness associated to in vivo conditions, as well as were more evenly distributed stomata. Those modifications did not qualitatively affect the production of medicinal metabolites and biopesticides. Terpenes and tannins were observed in specialized cells called idioblasts, located in the mesophyll and in the midrib region, respectively. Thus, in a qualitative approach, we can affirm that the different environments do not modify metabolites production. Increased production of these bioactive compounds could be achieved by isolation and in vitro culture of idioblasts as a new source of research in plant biotechnology.

CO2 enrichment alters morphophysiology and improves growth and acclimatization in Etlingera Elatior (Jack) R.M. Smith micropropagated plants

“…For micromorphometric analysis, three images of different plants were obtained per treatment, in which epidermal thickness (adaxial and abaxial surfaces), mesophyll thickness, and total thickness of the leaf blade were measured, as proposed by Rodrigues et al (2020) with modifications. Three measurements of each parameter per image were taken using a photomicroscope (AX70TRF; Olympus Optical, Tokyo, Japan) equipped with a U-Photo system and processed in ImageJ® software.…”