Leaf and canopy photosynthesis of a chlorophyll deficient soybean mutant

Sakowska, Karolina; Alberti, Giorgio; Genesio, L.; Peressotti, Alessandro; Vedove, Gemini Delle; Gianelle, Damiano; Colombo, R.; Rodeghiero, Mirco; Panigada, Cinzia; Juszczak, Radosław; Celesti, Marco; Rossini, Micol; Haworth, Matthew; Campbell, Ben; Mévy, Jean-Philippe; Vescovo, L.; Cendrero-Mateo, Maria Pilar; Rascher, Uwe; Miglietta, Franco

doi:10.1111/pce.13180

Cited by 80 publications

(66 citation statements)

References 45 publications

(81 reference statements)

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“…Chlorophyll levels are correlated with the rate of photosynthesis in plants (Huang et al 2009). 551 Some controversy has been reported in mutants of soybean affected in an ortholog of oy1 with 552 some reports showing little impact on photosynthesis (Sakowska et al 2018) and others clearly 553 demonstrating a linear relationship between chlorophyll variation and photosynthesis (Walker et 554 al. 2017).…”

Section: Defoliation Of Zea Mays Sorghum Bicolor and Setaria Viridimentioning

confidence: 99%

Interaction between induced and natural variation atoil yellow1delays reproductive maturity in maize

Khangura¹,

Venkata²,

Marla³

et al. 2019

Preprint

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24We previously demonstrated that maize (Zea mays) locus very oil yellow1 (vey1) encodes a 25 putative cis-regulatory expression polymorphism at the magnesium chelatase subunit I gene (aka 26 oil yellow1) that strongly modifies the chlorophyll content of the semi-dominant Oy1-N1989 27 mutants. The vey1 allele of Mo17 inbred line reduces chlorophyll content in the mutants leading 28 to reduced photosynthetic output. Oy1-N1989 mutants in B73 reached reproductive maturity four 29 days later than wild-type siblings. Enhancement of Oy1-N1989 by the Mo17 allele at the vey1 QTL 30 delayed maturity further, resulting in detection of a flowering time QTL in two bi-parental 31 mapping populations crossed to Oy1-N1989. The near isogenic lines of B73 harboring the vey1 32 allele from Mo17 delayed flowering of Oy1-N1989 mutants by twelve days. Just as previously 33 observed for chlorophyll content, vey1 had no effect on reproductive maturity in the absence of 34 the Oy1-N1989 allele. Loss of chlorophyll biosynthesis in Oy1-N1989 mutants and enhancement 35 by vey1 reduced CO2 assimilation. We attempted to separate the effects of photosynthesis on the 36 induction of flowering from a possible impact of chlorophyll metabolites and retrograde signaling 37 by manually reducing leaf area. Removal of leaves, independent of the Oy1-N1989 mutant, 38 delayed flowering but surprisingly reduced chlorophyll contents of emerging leaves. Thus, 39 defoliation did not completely separate the identity of the signal(s) that regulates flowering time 40 from changes in chlorophyll content in the foliage. These findings illustrate the necessity to explore 41 the linkage between metabolism and the mechanisms that connect it to flowering time regulation. 42 43

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Section: Defoliation Of Zea Mays Sorghum Bicolor and Setaria Viridimentioning

confidence: 99%

Interaction between induced and natural variation atoil yellow1delays reproductive maturity in maize

Khangura¹,

Venkata²,

Marla³

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…Although chlorophyll biosynthesis is relatively simple, its regulation, including that of its degradation, their effects on photosynthesis, and how they play a role in the light system remain unknown, thus warranting further study. Chlorophyll-de cient mutants are ideal materials for the exploration of the mechanisms of photosynthesis and chlorophyll synthesis [26,27]; furthermore, many related genes have been identi ed [28][29][30]. However, the molecular mechanism underlying the effects of chlorophyll de ciency in chlorophyll-de cient mutants of Chinese cabbage remains unclear.…”

Section: Introductionmentioning

confidence: 99%

iTRAQ-based Proteomic Analysis of a Chlorophyll-Deficient Mutant Caused by Single Base Change in RPS4 of Chinese Cabbage (Brassica Campestris L. ssp. Pekinensis)

Tang

Shi

Wang

et al. 2020

Preprint

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Background: Plastids are important plant-cell organelles containing a genome and bacterial-type 70S ribosomes—primarily composed of plastid ribosomal proteins and ribosomal RNAs. In this study, a chlorophyll-deficient mutant (cdm) obtained from double-haploid Chinese cabbage ‘FT’ was identified as a plastome mutant with an A-to-C base substitution in the plastid gene encoding the ribosomal protein RPS4. To further elucidate the function and regulatory mechanisms of RPS4, a comparative proteomic analysis was conducted between cdm and ‘FT’ plants using isobaric tags and a relative and absolute quantitation by (iTRAQ)-based strategy.Results: A total of 6,245 proteins were identified, 540 of which were differentially expressed (DEPs) in the leaves of cdm as compared to those of ‘FT’—including 233 upregulated and 307 downregulated proteins. Upregulated DEPs were mainly involved in translation, organic nitrogen synthesis, ribosomes, and spliceosomes. Meanwhile, downregulated DEPs were mainly involved in photosynthesis, photosynthetic reaction centres, photosynthetic light harvesting, carbon fixation, and chlorophyll binding. Our findings indicate an important role of RPS4 in the regulation of growth and development of Chinese cabbage, possibly by regulating plastid translation activity by affecting the expression of specific photosynthesis- and cold stress-related proteins. Moreover, a multiple reaction monitoring test and quantitative real time polymerase chain reaction analysis confirmed our iTRAQ results.Conclusions: Quantitative proteomic analysis allowed us to confirm diverse changes in the metabolic pathways between cdm and ‘FT’ plants. Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes enrichment analyses revealed that DEPs were significantly associated with photosynthesis, chlorophyll metabolism, carbon metabolism, RNA transport, glucosinolate biosynthesis, and gene splicing. This work provides new insights into the regulation of chlorophyll biosynthesis and photosynthesis in Chinese cabbage.

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“…Chlorophyll levels are correlated with the rate of photosynthesis in plants (Huang et al 2009). Some controversy has been reported in mutants of soybean affected in an ortholog of oy1 with some reports showing little impact on photosynthesis (Sakowska et al 2018) and others clearly demonstrating a linear relationship between chlorophyll variation and photosynthesis (Walker et al 2017). The reasons for conflicting conclusions results from soybean mutants are not fully clear but it warrants some caution in making simplistic interpretations about the impact of chlorophyll deficient mutants on photosynthesis.…”

mentioning

confidence: 99%

Interaction Between Induced and Natural Variation at oil yellow1 Delays Reproductive Maturity in Maize

Khangura

Venkata²,

Marla³

et al. 2020

G3 Genes|Genomes|Genetics

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We previously demonstrated that maize (Zea mays) locus very oil yellow1 (vey1) encodes a putative cis-regulatory expression polymorphism at the magnesium chelatase subunit I gene (aka oil yellow1) that strongly modifies the chlorophyll content of the semi-dominant Oy1-N1989 mutants. The vey1 allele of Mo17 inbred line reduces chlorophyll content in the mutants leading to reduced photosynthetic output. Oy1-N1989 mutants in B73 reached reproductive maturity four days later than wild-type siblings. Enhancement of Oy1-N1989 by the Mo17 allele at the vey1 QTL delayed maturity further, resulting in detection of a flowering time QTL in two bi-parental mapping populations crossed to Oy1-N1989. The near isogenic lines of B73 harboring the vey1 allele from Mo17 delayed flowering of Oy1-N1989 mutants by twelve days. Just as previously observed for chlorophyll content, vey1 had no effect on reproductive maturity in the absence of the Oy1-N1989 allele. Loss of chlorophyll biosynthesis in Oy1-N1989 mutants and enhancement by vey1 reduced CO2 assimilation. We attempted to separate the effects of photosynthesis on the induction of flowering from a possible impact of chlorophyll metabolites and retrograde signaling by manually reducing leaf area. Removal of leaves, independent of the Oy1-N1989 mutant, delayed flowering but surprisingly reduced chlorophyll contents of emerging leaves. Thus, defoliation did not completely separate the identity of the signal(s) that regulates flowering time from changes in chlorophyll content in the foliage. These findings illustrate the necessity to explore the linkage between metabolism and the mechanisms that connect it to flowering time regulation.

show abstract

Leaf and canopy photosynthesis of a chlorophyll deficient soybean mutant

Cited by 80 publications

References 45 publications

Interaction between induced and natural variation atoil yellow1delays reproductive maturity in maize

Interaction between induced and natural variation atoil yellow1delays reproductive maturity in maize

iTRAQ-based Proteomic Analysis of a Chlorophyll-Deficient Mutant Caused by Single Base Change in RPS4 of Chinese Cabbage (Brassica Campestris L. ssp. Pekinensis)

Interaction Between Induced and Natural Variation at oil yellow1 Delays Reproductive Maturity in Maize

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