Leaf and Floral Parts Feeding by Orange Tip Butterfly Larvae Depends on Larval Position but Not on Glucosinolate Profile or Nitrogen Level

Abstract

In an attempt to identify chemical signals governing the general flower and silique feeding behavior of larvae of the orange tip butterfly, Anthocharis cardamines (L.), we investigated feeding behavior and chemistry of two major host plants: Cardamine pratensis L. and Alliaria petiolata (Bieb.) Cavara & Grande (garlic mustard). Larvae reportedly feed mainly on flowers and siliques rather than leaves in nature, and did so when observed on the original host plants. Behavioral experiments, using detached A. petiolata branches, however, showed that larvae readily accepted leaves and only the final instar showed a tendency for directed movement towards floral parts. To search for semiochemicals that control plant part preference and to assess possible nutritional consequences of floral parts feeding, we determined glucosinolate profiles and total nitrogen levels of floral parts and leaves. There was only moderate difference between glucosinolate profiles of leaves and floral parts within each of two host plant species. In contrast, the profiles of floral parts differed significantly between them. A. petiolata was dominated by 2-propenyl glucosinolate, while C. pratensis was dominated by aromatic glucosinolates and branched aliphatic glucosinolates, with considerable variation among populations. Nitrogen levels tended to be higher in floral parts than in leaves in A. petiolata, but not in C. pratensis, so floral feeding could not generally be attributed to higher N content. With the exception of a tendency of last instar larvae (L5) to move to the apex and ingest flowers and upper stem, we did not find either a plant chemistry basis or larval acceptance/rejection behavior that could explain the usual feeding of floral parts by orange tip larvae of all instars. However, by artificial manipulation of vertical larval position on host plants, we found that the frequency of leaf vs. flower feeding during 24 hr depended significantly on the initial larval position. Hence, we suggest that the placement of eggs on floral parts by ovipositing female butterflies is a major explanation of orange tip feeding habits previously known from field observations.

Uncovering different parameters influencing florivory in a specialist herbivore

“…The pronounced influence of the initial position on the likelihood to reach the flowers, as found for A. rosae larvae in the current study, has been also reported for A. cardamines caterpillars on detached stems of their host plants (Agerbirk et al, 2010). The pronounced influence of the initial position on the likelihood to reach the flowers, as found for A. rosae larvae in the current study, has been also reported for A. cardamines caterpillars on detached stems of their host plants (Agerbirk et al, 2010).…”

“…The distribution of physiological and chemical plant traits may have a subordinate function in driving florivory (Agerbirk et al, 2010;Carmona et al, 2011), but these traits certainly influence the feeding pattern and performance of herbivores. Perception of plant cues and the ability of herbivores to move on the plant surface, which depends on the plant species-specific morphological structures of waxes and trichomes, seem to be superordinate traits influencing the probability of florivory.…”

“…The females of this species lay their eggs on the leaves (M. A. The initial position of larvae of this butterfly influences leaf and flower feeding on detached branches of Alliaria petiolata (Bieb) (Agerbirk et al, 2010). obs.).…”

“…Abdalsamee and C. Müller, pers. However, the differences could not simply be explained by the distribution of chemical characteristics, namely the glucosinolates (GSs) and myrosinases, in flowers versus leaves of Brassicaceae (Agerbirk et al, 2010;Bandeili & Müller, 2010). By contrast, females of Anthocharis cardamines L. (Lepidoptera: Pieridae) lay their eggs on young inflorescences (Wiklund & Friberg, 2009).…”

Florivory as an Opportunity Benefit of Aposematism

“…If the animal is cryptic when small, thenfrom the arguments above-it should feed on interior leaves during initial instars (Smallegange et al 2007;Agerbirk et al 2010;Bandeili and Müller 2010). Thus, larval florivores should be later instars, should be chemically defended, and should advertise those defenses.…”