Leaf Assisted Green Synthesis of Silver Nanoparticles from Syzygium Alternifolium (Wt.) Walp. Characterization and Antimicrobial Studies

Abstract

Development of environmentally benign methods for synthesis of nanoparticles is an evolving important branch of nanotechnology. In the present study we report a green method for synthesis of stable silver nanoparticles (SNPs) from aqueous leaf extract of Syzygium alternifolium an endemic medicinal plant to Southern Eastern Ghats. These green synthesized nanoparticles are characterized by using UV-VIS spectroscopy, FTIR, XRD, AFM, SEM with EDAX and TEM. The colour change from yellow to grey is observed upon synthesis and 448 nm peak was obtained from UV-VIS spectroscopic analysis. FTIR spectroscopic studies confirms that phenols and proteins of leaf extract is main responsible for capping and stabilization of these SNPs. Crystallographic studies from XRD indicated the SNPs are crystalline in nature and owing 44 nm size. High resolution and magnification studies with AFM, SEM and TEM analysis revealed that the nanoparticles are spherical in shape having the size range from 7 to 44 nm. EDAX pattern of synthesized SNPs showed 47.28 weight percentage of Ag metal in the sample indicate the purity of sample. Further, the antimicrobial studies of these green synthesized SNPs show high toxicity towards different bacterial and fungal isolates. The results revealed that the selected medicinal plant possess the potentiality towards the synthesis of narrow range nanoparticles also combat with the pathogens.

Synthesis, characterization and antimicrobial studies of bio silica nanoparticles prepared from Cynodon dactylon L.: a green approach

“…The liquid fraction was discarded and the pellet containing SiO 2 was washed several times with distilled water. The content was stirred with 1 M NaOH to form sodium silicate and water molecules (2). The mixture was suction pumped and titrated with 3 M HNO 3 to get the pure form of SiO 2 , sodium nitrate and water molecules (3).…”

“…These approaches are complicated, expensive and cause potential environmental and biological hazards [1]. Green synthesis of nanoparticles with the help of plants as reducing agents is an efficient, cost effective, fast and eco-friendly in manner [2]. In recent past, most of the scientists adopted green synthesis methods for the production of narrow-ranged particles, like calcium [3], copper [4], gold [5], iron [6], silica [7], silver [8] and zinc [9] from different medicinal plants.…”

Biological synthesis of silver nanoparticles from Adansonia digitata L. fruit pulp extract, characterization, and its antimicrobial properties

“…Silver is one among the metal nanoparticles focused much interest due to its wide variety of applications [ 8 ]. It has different biological activities such as antimicrobial [ 9 ], anthelmintic [ 10 ], antilarvicidic [ 11 ], antioxidant [ 12 ], anticancer [ 13 ], anti-inflammatory [ 14 ], hepatoprotective [ 15 ], and wound healing activity [ 10 ]. Conventional methods for synthesizing AgNPs are mainly by chemical, physical, and microbe-mediated synthesis.…”

Green Silver Nanoparticles: Novel Therapeutic Potential for Cancer and Microbial Infections

“…As shown in Table 1 [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27], the antimicrobial effect of the green silver nanoparticles, mainly their antibacterial [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25] and antifungal action [10,[12][13][14]17,18,[23][24][25], has been widely reported. It is important to mention that despite a wide variety of studies existing in the literature, antimicrobial activity has generally been evaluated in the same microorganisms even though there is a large amount of bacteria, fungi and viruses of clinical importance that could be studied.…”

“…These tests provide crucial information on the ranges of safety and cytotoxicity of the biosynthetized nanoparticles, information that result fundamental to can be designed future experiments with biomedical applications in preclinical and clinical models. Another important fact is about the physical nature of the nanoparticles with respect to their size, most of the studies obtained nanoparticles with sizes greater than 10 nm in diameter [8,[10][11][12][13][14][15][16][17][19][20][21][22][23][24][25][26][27]; it would be interesting to know their effects with sizes smaller than 10 nm since there is evidence that their biological effects also dependent on size [28]. Furthermore, for a better comparative analysis of the therapeutic potential of green silver nanoparticles, it is important to include in future experiments reference drugs that are currently the most used and effective in antimicrobial therapy in humans (cephalosporins, quinolones, and macrolides) [29], since most studies existing do not include used-commercial drugs as control [8][9]11,[13][14][15][16]18,[20][21] or it is included drugs used in the past.…”