Leaf extract of Eugenia uniflora L. prevents episodic memory impairment induced by streptozotocin in rats

Silva, Ilton Santos da; Corbellini, Joana Rosar; Pfutzenreuter, Gabrielle; Maranho, Leila Teresinha; Pincerati, Márcia Regina

doi:10.4103/pr.pr_37_19

Cited by 5 publications

(3 citation statements)

References 22 publications

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“…After this procedure, the maze was cleaned with alcohol 70% and a retest was conducted with inter-trial-interval (ITI) of 5 min. A retest 5 min after the first exposure has shown that rats are able to use working memory, a well-known hippocampal dependent cognitive function, to acquire and remember about the aversive memory represented by the exposure to an open and elevated environment such as the EPM in the first exposure (Da Silva et al, 2019).…”

Section: Elevated Plus Mazementioning

confidence: 99%

Intracerebroventricular streptozotocin induces behavioral impairments and increases short-term C3 gene expression in the hippocampus of Wistar rats

Pfutzenreuter¹,

Nieradka²,

Pincerati³

et al. 2020

Acta Neurobiologiae Experimentalis

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A non-transgenic rat model based on intracerebroventricular injection of streptozotocin (STZ) has been used as an animal model to investigate mechanisms associated to the late onset of sporadic Alzheimer's disease, such as anatomical and behavioral impairments. However, molecular aspects related to gene expression, mainly in the hippocampus, require more investigation. Thus, this study evaluated the early and late cognitive functions and hippocampal gene expression after STZ administration. Male Wistar rats were divided into 4 groups: STZ (injected bilaterally), control group for the early memory function evaluation (1 month after surgery = phase 1, same volume of vehicle), and the same treatment for the late memory function evaluation (4 months after surgery = phase 2). The animals were observed in the elevated plus maze to assess behaviors related to anxiety, risk-assessment and fear-related memories. The behavioral tests were followed by brain removal and hippocampal dissection for RNA extraction and qRT-PCR to assess the expression levels of 4 Alzheimer's disease related genes: Mapt, Apoe, C3 and Ps-1. Animals from both phases showed increased time percentage and number of entries into the open arms, indicating risk behavior associated with anxiety, and an increased time percentage in the center square for both exposures (re-test) when compared to the control group, suggesting working memory impairment related to an aversive event. Statistical analyses indicated that the STZ group presented alterations in anxiety, memory and risk assessment responses. Additionally, one month after STZ administration, C3 gene assays revealed an increased expression. Therefore, current data indicate that neuroinflammatory events linked to the expression of pro inflammatory cytokines such as C3 are related to memory, anxiety and decision-making alterations.

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Section: Elevated Plus Mazementioning

confidence: 99%

Intracerebroventricular streptozotocin induces behavioral impairments and increases short-term C3 gene expression in the hippocampus of Wistar rats

Pfutzenreuter¹,

Nieradka²,

Pincerati³

et al. 2020

Acta Neurobiologiae Experimentalis

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“…6,7 The infusion of its leaves is popularly used as an alternative for the treatment and control of fever, 8-10 sore throat and inammation, 11,12 diarrhoea and digestive symptoms. 13,14 In addition, several biological activities related to the leaves of E. uniora are reported in the literature [8][9][10][11][15][16][17][18][19][20] and most of the biological activities are related to the presence of avonoids 8,11,[21][22][23] and tannins 15,16,24 in their leaves. Among these are the hydrolysable tannins hippomanin A/gemin D (C 27 H 22 O 18 ), 25,26 desmanthin I (C 28 H 24 O 16 ) 25 and oenothein B (C 68 H 48 O 44 ) 25 in addition to the avonoids myricitrin (C 21 H 20 O 12 ) 25,26 and its aglycone myricetin (C 15 H 10 O 8 ).…”

Section: Introductionmentioning

confidence: 99%

ESI(−)FT-ICR MS for the determination of best conditions for producing extract abundant in phenolic compounds from leaves of E. uniflora and FTIR-PCA as a sample screening method

de Oliveira,

Lyrio,

Filgueiras

et al. 2024

Anal. Methods

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“…It is distributed throughout the world's tropical and subtropical regions [23,24]. It has a long history in traditional medicine all over the world as treatment for diarrhea, diabetes, cough, stomach pain, dysentery, toothache, indigestion, constipation, fever, and wound healing; it is notable that different parts, such as leaves, flowers and barks are involved in traditional uses in the form of decoction and infusions [25][26][27]. Another point is the outstanding pharmacological properties of P. guajava, including, but not limited to, antioxidant, anti-inflammatory, antimicrobial, cytotoxic, analgesic, cardioprotective, hepato-protective, and antidiabetic activities [28][29][30][31].…”

Section: Introductionmentioning

confidence: 99%

Chemical Constituents, Antioxidant, and Enzyme Inhibitory Activities Supported by In-Silico Study of n-Hexane Extract and Essential Oil of Guava Leaves

et al. 2022

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Psidium guajava (Guava tree) is one of the most widely known species in the family Myrtaceae. The Guava tree has been reported for its potential antioxidant, anti-inflammatory, antimicrobial, and cytotoxic activities. In the current study, the chemical compositions of the n-hexane extract and the essential oil of P. guajava were investigated using the GC/MS analysis, along with an evaluation of their antioxidant potential, and an investigation into the enzyme inhibition of acetylcholinesterase (AChE), butyrylcholinesterase (BchE), tyrosinase, α-amylase, and α-glucosidase. Moreover, molecular docking of the major identified active sites of the target enzymes were investigated. The chemical characterization of the n-hexane extract and essential oil revealed that squalene (9.76%), α-tocopherol (8.53%), and γ-sitosterol (3.90%) are the major compounds in the n-hexane extract. In contrast, the major constituents of the essential oil are D-limonene (36.68%) and viridiflorol (9.68%). The n-hexane extract showed more antioxidant potential in the cupric reducing antioxidant capacity (CUPRAC), the ferric reducing power (FRAP), and the metal chelating ability (MCA) assays, equivalent to 70.80 ± 1.46 mg TE/g, 26.01 ± 0.97 mg TE/g, and 24.83 ± 0.35 mg EDTAE/g, respectively. In the phosphomolybdenum (PM) assay, the essential oil showed more antioxidant activity equivalent to 2.58 ± 0.14 mmol TE/g. The essential oil demonstrated a potent BChE and tyrosinase inhibitory ability at 6.85 ± 0.03 mg GALAE/g and 61.70 ± 3.21 mg KAE/g, respectively. The α-amylase, and α-glucosidase inhibitory activity of the n-hexane extract and the essential oil varied from 0.52 to 1.49 mmol ACAE/g. Additionally, the molecular docking study revealed that the major compounds achieved acceptable binding scores upon docking with the tested enzymes. Consequently, the P. guajava n-hexane extract and oil can be used as a promising candidate for the development of novel treatment strategies for oxidative stress, neurodegeneration, and diabetes mellitus diseases.

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Leaf extract of Eugenia uniflora L. prevents episodic memory impairment induced by streptozotocin in rats

Cited by 5 publications

References 22 publications

Intracerebroventricular streptozotocin induces behavioral impairments and increases short-term C3 gene expression in the hippocampus of Wistar rats

Intracerebroventricular streptozotocin induces behavioral impairments and increases short-term C3 gene expression in the hippocampus of Wistar rats

ESI(−)FT-ICR MS for the determination of best conditions for producing extract abundant in phenolic compounds from leaves of E. uniflora and FTIR-PCA as a sample screening method

Chemical Constituents, Antioxidant, and Enzyme Inhibitory Activities Supported by In-Silico Study of n-Hexane Extract and Essential Oil of Guava Leaves

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