Leaf Extract of Syzygium cumini Shows Anti-Vibrio Activity Involving DNA Damage

Ahsan, Nazmul; Paul, Nilanjana; Islam, Nazrul; Akhand, Anwarul A.

doi:10.3329/dujps.v11i1.12483

Cited by 11 publications

(9 citation statements)

References 10 publications

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“…Pathogenic bacteria like E. coli, S. aureus and K. pneumonia on treatment with Caesalpinia coriaria glycosides and flavonoids for 24 h showed a smear of fragmented DNA (Anandhi et al 2014 ). Similarly the ethanolic leaf extract of S. cumini after treating V. cholerae showed fragmented DNA after 3 h of treatment (Ahsan et al 2012 ).…”

Section: Discussionmentioning

confidence: 97%

Antimicrobial action of methanolic seed extracts of Syzygium cumini Linn. on Bacillus subtilis

et al. 2017

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Phytochemicals of Syzygium cumini are used for the treatment of various diseases as a traditional medicine but the mechanism behind their action is not well reported. Antimicrobial activity of methanolic seed extract of S. cumini was done by agar well diffusion assay on Bacillus subtilis and its zone of inhibition was found to be 20.06 mm in comparison to control having no zone of inhibition. MIC of S. cumini was found to be 0.3 mg/ml. Genomic DNA degradation of B. subtilis reveals apoptosis and FE-scanning electron microscope indicates cell wall cracking on several intervals of time. Results of propidium iodide staining showed few bacterial cells were stained in control; however population of stained cells increased after exposing them for varying period of time. Flow cytometric kinetic data analysis on the membrane permeabilization in bacterial cell showed the significant contribution of antimicrobial potential of the seed extract on antimicrobial-induced permeabilization. In silico analysis revealed two components of S. cumini methanolic extract to be active against four enzymes (PDB ID—1W5D, 4OX3, 3MFD and 5E2F) which are crucial for plasma membrane synthesis in B. subtilis. Moreover lupeol showed highest binding energy for macromolecule 1W5D and 4OX3 forming one hydrogen bond each whereas stigmasterol showed the highest binding energy for macromolecule 3MFD and 5E2F forming four hydrogen bonds and alkyl bonds respectively. It demonstrates that methanolic seed extracts of S. cumini could be used for inhibition of food born infection caused by B. subtilis and also an alternative of prevalent antibiotics.

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Section: Discussionmentioning

confidence: 97%

Antimicrobial action of methanolic seed extracts of Syzygium cumini Linn. on Bacillus subtilis

et al. 2017

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“…50 The ethanol extract of Jamun leaf has been reported to be active against the Vibrio cholerae serogroups Ogawa and Inaba. 51 The 70% ethanol extract of Jamun leaf, bark, pulp and seed showed a potent antimicrobial activity against various Gram-positive bacteria (including B. subtilis, B. cereus and S. aureus) and Gram-negative bacteria (including S. flexneri, P. aeruginosa and V. cholera). Comparison analysis showed that the leaf and bark extracts were more potent than the pulp and seed extracts.…”

Section: Antibacterial and Antifungal Activitymentioning

confidence: 99%

Phytochemical Composition and Pleotropic Pharmacological Properties of Jamun, Syzygium Cumini Skeels

Jagetia¹

2017

Journal of Exploratory Research in Pharmacology

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Plants have been employed as medicine since time immemorial, and there has been a recent resurgence in the use of plants as medicines due to their little or no toxicity at the doses used for treatment of different ailments. This review discusses in detail the phytochemical and pharmacological activities of Jamun (Syzygium cumini), a tree belonging to family Myrtaceae, which has been credited with several medicinal properties in the traditional system of medicine, the Ayurveda. The different properties attributed to Jamun are sweet, sour, astringent, acrid, refrigerant, carminative, diuretic, and digestive. Research and practical use in traditional medicinal systems have found Jamun to be effective in treating leucorrhoea, gastric disorders, fever, diabetes, piles, stomachache, wounds, and dental, digestive and skin disorders. Some compounds in Jamun have antioxidant, antimicrobial, antiallergic, antidiabetic, antihyperlipidemic, anticancer, gastroprotective, hepatoprotective, cardioprotective and radioprotective activity. Finally, Jamun has been found to contain phytochemicals including anthroquinones, alkaloids, catechins, flavonoids, glycosides, steroids, phenols, tannins, saponins and cardiac glycosides. The diverse activities of Jamun may be due to its abilities to scavenge free radicals, increase antioxidant status of cells by increasing glutathione, glutathione peroxidase, catalase and/or superoxide dismutase, and to attenuate lipid peroxidation. In addition, it also suppresses the transcription of peroxisome proliferator-activated receptor, Nuclear factor kappa B, cyclooxygenase, inducible nitric oxide synthase, tumor necrosis factor alpha and other proinflammatory cytokines, accompanied by the up-regulation of nuclear factor erythroid 2-related factor 2 transcription, which is involved in regulating the antioxidant status of the cells.

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“…Gel was observed under UV light and photographs were taken using gel documentation system. This test was done to observe whether the extract could cause fragmentation and degradation of Leptospira genomic DNA (Ahsan et al, 2012).…”

Section: Genomic Dna Damagementioning

confidence: 99%

“…There are many plant extracts that have shown to cause cell death involving bacterial DNA fragmentation. For example, the ethanol extract of Syzygium cumini leave has caused fragmentation of genomic DNA of Vibrio cholerae (Ahsan et al, 2012). Methanol and aqueous extracts from Eclipta alba and Phyllanthus amarus also resulted in fragmentation of the genome DNA of Leptospira sp.…”

Section: Genomic Dna Damagementioning

confidence: 99%

In-vitro antileptospiral activity of Canarium odontophyllum Miq. (Dabai) leaves extract

Ishak¹,

Ariffudin

Azmi

et al. 2019

MJM

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Aims: To evaluate the anti-leptospiral activity of Canarium odontophyllum leaves against Leptospira interrogans serovar Bataviae and Leptospira borgpetersenii serovar Javanica. Methodology and results: The extracts (hexane, acetone, methanol and aqueous) used in this study were tested at concentration ranging from 0.049 mg/mL to 25 mg/mL using broth microdilution method. Percentage inhibition (%) was obtained through OD reading at 400 nm. Only methanol extract was incubated with Leptospira to observe population changes under dark field microscope prior to subjected for DNA damaging studies through gel electrophoresis of genomic DNA. Methanol extract showed the highest percentage inhibition of 66% against L.interrogans serovar Bataviae and 74% against L. borgpetersenii serovar Javanica. The IC50 value of methanol extract was 4.60 mg/mL and 2.25 mg/mL against serovar Bataviae and serovar Javanica, respectively. Both Leptospira culture which was treated with IC50 value of methanol extract showed drastic decrease in population compared to untreated Leptospira for both serovar. There was no DNA damage towards serovar Bataviae. However, serovar Javanica exhibited DNA damage as observed from the presence of DNA fragmentation on the gel electrophoresis. Conclusion, significance and impact of study: These findings confirmed that methanol leaves extract from of Canarium odontophyllum has a potential to control leptospirosis.

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Leaf Extract of Syzygium cumini Shows Anti-Vibrio Activity Involving DNA Damage

Cited by 11 publications

References 10 publications

Antimicrobial action of methanolic seed extracts of Syzygium cumini Linn. on Bacillus subtilis

Antimicrobial action of methanolic seed extracts of Syzygium cumini Linn. on Bacillus subtilis

Phytochemical Composition and Pleotropic Pharmacological Properties of Jamun, Syzygium Cumini Skeels

In-vitro antileptospiral activity of Canarium odontophyllum Miq. (Dabai) leaves extract

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