Leaf Extracts of Cistus ladanifer Exhibit Potent Antioxidant and Antiproliferative Activities against Liver, Prostate and Breast Cancer Cells

Bouothmany, Kaoutar; Bourhia, Mohammed; Aoussar, Noura; Attaleb, Mohammed; Salamatullah, Ahmad Mohammad; Nafidi, Hiba-Allah; Mellouki, Fouad; Mzibri, Mohammed El; Aboul‐Soud, Mourad A. M.; Benbacer, Laïla

doi:10.3390/app12178603

Cited by 4 publications

(1 citation statement)

References 36 publications

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“…The evaluation of cancer cell growth inhibition was carried out based on the MTT colorimetric assay (37). The cell cultures were distributed in 96-well microplates at a density of 8000 cells per well.…”

Section: Cell Viability: Mtt Assaymentioning

confidence: 99%

Antioxidant, photoprotective, cytotoxic, and antimicrobial activities of Albuca amoena Batt.

Zakhour,

El-Guourrami,

Bouothmany

et al. 2024

J Herbmed Pharmacol

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Introduction: Albuca amoena Batt. is an endemic plant from Morocco used for treating melanoma or leishmaniasis. The aim of this study is to test the antioxidant, photoprotective, cytotoxic, and antimicrobial properties of this plant. Methods: The extracts of the aerial and bulbous parts were obtained by hydroalcoholic maceration of the plant. Their antioxidant potentials were evaluated in vitro using four methods of 2,2-diphenyl-1picrylhydrazil (DPPH), 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), ferric reducing antioxidant power (FRAP) assay, and total antioxidant capacity assay (TAC). The photoprotective properties were evaluated through the study of UVA and UVB absorbance and antityrosinase activity. The cytotoxic activity was determined against two breast cancer cell lines (MCF-7 and MDA-MB-231) and one human colorectal cancer cell line (HT-29) using the 3-[4,5-Dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) method. In addition, the antimicrobial activity was tested on six pathogens: Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Staphylococcus aureus, and Candida albicans by using the disc diffusion method. Results: The results showed that the UVA and UVB absorptive proprieties of the extracts were similar to those used as positive controls: Methyl salicylate and zinc oxide. Moreover, the antioxidant and antityrosinase properties of the studied extracts were found to be weakly active. The extracts had strong cytotoxicity activities, especially against HT-29 cell line. However, the extracts did not show any antibacterial activity. Conclusion: According to the results of this study, the extracts can be used as a sunscreen to avoid dermal anomalies and as a new source of cytotoxic bioactive compound.

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Section: Cell Viability: Mtt Assaymentioning

confidence: 99%

Antioxidant, photoprotective, cytotoxic, and antimicrobial activities of Albuca amoena Batt.

Zakhour,

El-Guourrami,

Bouothmany

et al. 2024

J Herbmed Pharmacol

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Hepatoprotective Effects of a Chemically-Characterized Extract from Cistus ladanifer: In Vivo and in Silico Investigations

Bouothmany,

Amrati,

Chebaibi

et al. 2024

Natural Product Communications

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Background: Liver damage is indicated by histopathological and metabolic dysfunction. There has been increasing interest in natural hepatoprotective agents from medicinal herbs. The medicinal plant Cistus ladanifer widely used in phytotherapy was selected for this study. Objectives: The work aims to investigate the hepatoprotective effects of Cistus ladanifer leaf extracts in vivo against the paracetamol-induced hepatotoxicity mice and in silico using the docking method. The chemical composition of the extracts was also studied. Materials and methods: The paracetamol-induced hepatotoxicity mice were treated with aqueous and methanolic extracts of Cistus ladanifer at two doses of 150 and 300 mg/kg body. The serum levels of aspartate aminotransferase, alanine aminotransferase, triglycerides, total cholesterol, urea, and creatinine in mice-induced hepatotoxicity were measured to evaluate the hepatoprotective activity of the extracts. The histopathological alterations were also investigated. The chemical profile of methanolic and aqueous C. ladanifer extracts was determined using GC-MS. The Human Cytochrome P450 2E1(CYP2E1) was presented as a protein target and the docking was studied using Maestro version 11.5 software developed by Schrodinger, LLC. Results: The administration of C. ladanifer extracts showed hepatic protection at an oral dose of 150 and 300 mg/kg for the aqueous extract and only at 150 mg/kg for the methanolic one. The aqueous extract at both doses and the methanolic extract at 150 mg/kg didn’t occur any biochemical or histological alterations. However, the methanolic extract showed hepatic toxicity at 300 mg/kg. Various compounds were identified in the methanolic and the aqueous extracts, probably responsible of the hepatoprotective activity. The in silico study has also shown that some molecules present in both extracts presented a good affinity in the active site of Cytochrome P450 2E1 (CYP2E1). Conclusion: The results deserve more attention as C. ladanifer can be considered a source of pharmacological compounds with hepatoprotective effects. Further detailed investigation is needed to deepen the study.

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Phytochemical Profile and In Vitro Cytotoxic, Genotoxic, and Antigenotoxic Evaluation of Cistus monspeliensis L. Leaf Extract

Al-Naqeb,

Zorzi,

Oldani

et al. 2024

IJMS

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Cistus monspeliensis L. (C. monspeliensis) is used in Italian folk medicine. This study was performed to determine genotoxic and antigenotoxic effects of C. monspeliensis leaf extract against mitomycin C (MMC) using an in vitro cytokinesis-block micronucleus assay (CBMN) in the Chinese Hamster Ovarian K1 (CHO-K1) cell line. The phytochemical composition of C. monspeliensis extract was evaluated using an untargeted metabolomic approach by employing UPLC-PDA-ESI/MS. The automated in vitro CBMN assay was carried out using image analysis systems with a widefield fluorescence microscope and the ImageStreamX imaging flow cytometer. The phytochemical profile of C. monspeliensis extract showed, as the most abundant metabolites, punicalagin, myricetin, gallocathechin, and a labdane-type diterpene. C. monspeliensis, at the tested concentrations of 50, 100, and 200 μg/mL, did not induce significant micronuclei frequency, thus indicating the absence of a genotoxic potential. When testing the C. monspeliensis extract for antigenotoxicity in the presence of MMC, we observed a hormetic concentration-dependent effect, where low concentrations resulted in a significant protective effect against MMC-induced micronuclei frequency, and higher concentrations resulted in no effect. In conclusion, our findings demonstrate that C. monspeliensis extract is not genotoxic and, at low concentration, exhibits an antigenotoxic effect. In relation to this final point, C. monspeliensis may act as a potential chemo-preventive against genotoxic agents.

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Leaf Extracts of Cistus ladanifer Exhibit Potent Antioxidant and Antiproliferative Activities against Liver, Prostate and Breast Cancer Cells

Cited by 4 publications

References 36 publications

Antioxidant, photoprotective, cytotoxic, and antimicrobial activities of Albuca amoena Batt.

Antioxidant, photoprotective, cytotoxic, and antimicrobial activities of Albuca amoena Batt.

Hepatoprotective Effects of a Chemically-Characterized Extract from Cistus ladanifer: In Vivo and in Silico Investigations

Phytochemical Profile and In Vitro Cytotoxic, Genotoxic, and Antigenotoxic Evaluation of Cistus monspeliensis L. Leaf Extract

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