Leaf me alone:<i>Medicago truncatula</i>defenses against foliar lepidopteran herbivores

Bede, Jacqueline C.

doi:10.1002/9781119409144.ch51

Cited by 2 publications

(2 citation statements)

References 138 publications

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“…Saponins are involved in protection of many plant species against pathogens and insect herbivores (Hussain et al, 2019;Roopashree and Naik, 2019;Bede, 2020;Zaynab et al, 2021). Foliar levels of oleanoic acid-derived saponins, such as the aglycone hederagenin, are correlated with caterpillar deterrence (Cai et al, 2017); however, whether this compound acts as a toxin or antifeedant is unclear.…”

Section: Introductionmentioning

confidence: 99%

“…The barrel medic Medicago truncatula, a forage crop in Australia and Mediterranean countries (Crawford et al, 1989;Howieson et al, 2000), has morphological and chemical defenses to protect itself against insect herbivores of which triterpenoid saponins are key defensive plant specialized metabolites (Bede, 2020). From the precursor b-amyrin, triterpenoid saponins can be classi ed into groups depending on the aglycone: soyasaponin, hederagenin, bayogenin, medicagenic acid, of which the last three are derived from oleanolic acid (Vincken et al, 2007;Moses et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Trichoplusia Ni Transcriptomic Responses to the Phytosaponin Aglycone Hederagenin: Sex-related Differences

CHEN,

LAFLEUR,

SMITH

et al. 2023

Preprint

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Many plant species, particularly legumes, protect themselves with saponins. Previously, a correlation was observed between levels of oleanolic acid-derived saponins, such as hederagenin, in the legume Medicago truncatula and caterpillar deterrence. Using concentrations that reflect the foliar levels, hederagenin did not have a toxic effect on 4th instar caterpillars of the cabbage looper Trichoplusia ni nor did it act as a feeding deterrent. However, female caterpillars consumed more diet than males, presumably to obtain the additional nutrients required for oogenesis, and are, thus, exposed to higher hederagenin levels. When fed diet with lower hederagenin levels, male caterpillars expressed more genes encoding trypsin-like (LOC113500509, LOC113501951, LOC113501953, LOC113501966, LOC113501965, LOC113499659, LOC113501950, LOC113501948, LOC113501957, LOC113501962, LOC113497819, LOC113501946, LOC113503910) and the cytochrome P450 6B2-like (LOC113493761). When fed the high hederagenin, male caterpillars expressed stress-responsive genes (LOC113503484, LOC113505107). In contrast, female caterpillars expressed high levels of the cytochrome P450 6B7-like (LOC113492289). Bioinformatic tools predict that cytochrome P450s could catalyze the oxygenation of hederagenin which would increase the hydrophilicity of the compound. Expression of the MFS transporter (LOC113492899) showed a hederagenin dose-dependent increase in gene expression suggesting that this transporter may be involved in saponin efflux. These sex-related differences in feeding and detoxification should be taken into consideration in insecticide evaluations to minimize pesticide resistance.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Trichoplusia Ni Transcriptomic Responses to the Phytosaponin Aglycone Hederagenin: Sex-related Differences

CHEN,

LAFLEUR,

SMITH

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

Trichoplusia ni Transcriptomic Responses to the Phytosaponin Aglycone Hederagenin: Sex-Related Differences

Chen,

Lafleur,

Smith

et al. 2024

J Chem Ecol

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Many plant species, particularly legumes, protect themselves with saponins. Previously, a correlation was observed between levels of oleanolic acid-derived saponins, such as hederagenin-derived compounds, in the legume Medicago truncatula and caterpillar deterrence. Using concentrations that reflect the foliar levels of hederagenin-type saponins, the sapogenin hederagenin was not toxic to 4th instar caterpillars of the cabbage looper Trichoplusia ni nor did it act as a feeding deterrent. Female caterpillars consumed more diet than males, presumably to obtain the additional nutrients required for oogenesis, and are, thus, exposed to higher hederagenin levels. When fed the hederagenin diet, male caterpillars expressed genes encoding trypsin-like proteins (LOC113500509, LOC113501951, LOC113501953, LOC113501966, LOC113501965, LOC113499659, LOC113501950, LOC113501948, LOC113501957, LOC113501962, LOC113497819, LOC113501946, LOC113503910) as well as stress-responsive (LOC113503484, LOC113505107) proteins and cytochrome P450 6B2-like (LOC113493761) at higher levels than females. In comparison, female caterpillars expressed higher levels of cytochrome P450 6B7-like (LOC113492289). Bioinformatic tools predict that cytochrome P450s could catalyze the oxygenation of hederagenin which would increase the hydrophilicity of the compound. Expression of a Major Facilitator Subfamily (MFS) transporter (LOC113492899) showed a hederagenin dose-dependent increase in gene expression suggesting that this transporter may be involved in sapogenin efflux. These sex-related differences in feeding and detoxification should be taken into consideration in insecticide evaluations to minimize pesticide resistance.

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Leaf me alone:Medicago truncatuladefenses against foliar lepidopteran herbivores

Cited by 2 publications

References 138 publications

Trichoplusia Ni Transcriptomic Responses to the Phytosaponin Aglycone Hederagenin: Sex-related Differences

Trichoplusia Ni Transcriptomic Responses to the Phytosaponin Aglycone Hederagenin: Sex-related Differences

Trichoplusia ni Transcriptomic Responses to the Phytosaponin Aglycone Hederagenin: Sex-Related Differences

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