Leaf mesophyll K+ and Cl− fluxes and reactive oxygen species production predict rice salt tolerance at reproductive stage in greenhouse and field conditions

Yong, Miing-Tiem; Solis, Celymar Angela; Rabbi, Barkat; Huda, Samsul; Liu, Rong; Zhou, Meixue; Shabala, Lana; Venkataraman, Gayatri; Shabala, Sergey; Chen, Zhong‐Hua

doi:10.1007/s10725-020-00619-y

Cited by 21 publications

(7 citation statements)

References 81 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Koshihikari (O. sativa subsp. japonica) (Liu et al, 2019;Yong et al, 2020), and a wild rice line, Dongxiang (O. rufipogon). The rice plants in the control and saline conditions were evaluated from the early vegetative stage (21 days after sowing) up until maturity in the field at Western Sydney University (Latitude: -33 35' 58.42" S and Longitude: 150 45' 5.11" E, Richmond, NSW, Australia).…”

Section: Plant Materials Experimental Design and Agronomical Traitsmentioning

confidence: 99%

Sodium sequestration confers salinity tolerance in an ancestral wild rice

Solis

Yong

Venkataraman

et al. 2021

Physiologia Plantarum

Self Cite

View full text Add to dashboard Cite

Wild rice Oryza rufipogon, a progenitor of cultivated rice Oryza sativa L., possesses superior salinity tolerance and is a potential donor for breeding salinity tolerance traits in rice. However, a mechanistic basis of salinity tolerance in this donor species has not been established. Here, we examined salinity tolerance from the early vegetative stage to maturity in O. rufipogon in comparison with a salt-susceptible (Koshihikari) and a salt-tolerant (Reiziq) variety of O. sativa. We assessed their phylogeny and agronomical traits, photosynthetic performance, ion contents, as well as gene expression in response to salinity stress. Salt-tolerant O. rufipogon exhibited efficient leaf photosynthesis and less damage to leaf tissues during the course of salinity treatment. In addition, O. rufipogon showed a significantly higher tissue Na + accumulation that is achieved by vacuolar sequestration compared to the salt tolerant O. sativa indica subspecies. These findings are further supported by the upregulation of genes involved with ion transport and sequestration (e.g. high affinity K + transporter 1;4 [HKT1;4], Na + /H + exchanger 1 [NHX1] and vacuolar H + -ATPase c [VHA-c]) in salt-tolerant O. rufipogon as well as by the close phylogenetic relationship of key salt-responsive genes in O. rufipogon to these in salt-tolerant wild rice species such as O. coarctata. Thus, the high accumulation of Na + in the leaves of O. rufipogon acts as a cheap osmoticum to minimize the high energy cost of osmolyte biosynthesis and excessive reactive oxygen species production. These mechanisms demonstrated that O. rufipogon has important traits that can be used for improving salinity tolerance in cultivated rice. K E Y W O R D S gene expression, ion transporters, Oryza rufipogon Griff, phylogeny, tissue Na + tolerance) is one of the most important agricultural crops and is the main source of carbohydrates for more than half of the world's population (FAO, 2019). Demand for the crop is increasing due to population growth and, to meet this demand, rice production will have to move to marginal lands many of which are salinized. Rice is susceptible to soil salinization, which is one of the major factors limiting its production (Ma et al., 2012(Ma et al., , 2018Kotula et al., 2020). Salinity stress damages leaves, reduces growth, delays panicle emergence and, most importantly, reduces yields (Caperta et al., 2020;Zeng & Shannon, 2000). A 50% yield loss is estimated at a salinity level of Celymar Angela Solis and Miing-Tiem Yong contributed equally to this study.

show abstract

Section: Plant Materials Experimental Design and Agronomical Traitsmentioning

confidence: 99%

Sodium sequestration confers salinity tolerance in an ancestral wild rice

Solis

Yong

Venkataraman

et al. 2021

Physiologia Plantarum

Self Cite

View full text Add to dashboard Cite

show abstract

“…Na + homeostasis strategies in leaves of salt-tolerant O. latifolia require further investigation. We previously reported a significant, positive correlation between net K + flux and salinity tolerance in O. sativa (Yong et al 2020). Here, except for O. coarctata, we extended this finding to wild Oryza species (Figs.…”

Section: Discussionmentioning

confidence: 89%

Proto Kranz-like leaf traits and cellular ionic regulation are associated with salinity tolerance in a halophytic wild rice

et al. 2022

Self Cite

View full text Add to dashboard Cite

Species of wild rice (Oryza spp.) possess a wide range of stress tolerance traits that can be potentially utilized in breeding climate-resilient cultivated rice cultivars (Oryza sativa) thereby aiding global food security. In this study, we conducted a greenhouse trial to evaluate the salinity tolerance of six wild rice species, one cultivated rice cultivar (IR64) and one landrace (Pokkali) using a range of electrophysiological, imaging, and whole-plant physiological techniques. Three wild species (O. latifolia, O. officinalis and O. coarctata) were found to possess superior salinity stress tolerance. The underlying mechanisms, however, were strikingly different. Na+ accumulation in leaves of O. latifolia, O. officinalis and O. coarctata were significantly higher than the tolerant landrace, Pokkali. Na+ accumulation in mesophyll cells was only observed in O. coarctata, suggesting that O. officinalis and O. latifolia avoid Na+ accumulation in mesophyll by allocating Na+ to other parts of the leaf. The finding also suggests that O. coarctata might be able to employ Na+ as osmolyte without affecting its growth. Further study of Na+ allocation in leaves will be helpful to understand the mechanisms of Na+ accumulation in these species. In addition, O. coarctata showed Proto Kranz-like leaf anatomy (enlarged bundle sheath cells and lower numbers of mesophyll cells), and higher expression of C4-related genes (e.g., NADPME, PPDK) and was a clear outlier with respect to salinity tolerance among the studied wild and cultivated Oryza species. The unique phylogenetic relationship of O. coarctata with C4 grasses suggests the potential of this species for breeding rice with high photosynthetic rate under salinity stress in the future.

show abstract

“…Therefore, maintaining a stable K + concentration in plant cytoplasm under high Na + concentration is a key factor for plants to resist salt stress. Furthermore, leaf mesophyll K + and Cl ¯ fluxes and ROS production can be used to predict rice salt tolerance at the reproductive stage in the greenhouse and field conditions [ 67 ]. Under NaCl stress, AsA could reduce Na + and Cl ¯ , and increase K + , simultaneously enhance the antioxidant system, thereby improving the salt-tolerance of alfalfa.…”

Section: Discussionmentioning

confidence: 99%

Effects of exogenous ascorbic acid on seed germination and seedling salt-tolerance of alfalfa

2021

View full text Add to dashboard Cite

Alfalfa (Medicago sativa L.) is an important legume crop for forage, agriculture, and environment in the world. Ascorbic acid (AsA) plays positive roles in plants. However, its effects on germination and salt-tolerance of alfalfa are unknown. The effects of AsA applications on seed germination and seedling salt-tolerance of alfalfa were investigated. The results revealed that 0.1 and 1 mmol L-1 of exogenous AsA increased germination, amylase, and protease, as well as seedling length, fresh weight (FW), dry weight (DW), and endogenous AsA both in the shoots and roots, except that 1 mmol L-1 AsA reduced the activities of α-amylase, β-amylase and protease on day 3. However, 10 and 100 mmol L-1 AsA inhibited these parameters and even caused serious rot. It indicates that 0.1 mmol L-1 AsA has the optimal effects, whereas 100 mmol L-1 AsA has the worst impacts. Another part of the results showed that 0.1 mmol L-1 AsA not only enhanced stem elongation, FW and DW, but also increased chlorophyll and carotenoids both under non-stress and 150 mmol L-1 NaCl stress. Furthermore, 0.1 mmol L-1 AsA mitigated the damages of membrane permeability, malondialdehyde, and excessive reactive oxygen species (ROS) and ions both in the shoots and roots under 150 mmol L-1 NaCl stress. Hence, 0.1 mmol L-1 AsA improves growth and induces salt-tolerance by inhibiting excessive ROS, down-regulating the ion toxicity and up-regulating the antioxidant system. The principal component analysis included two main components both in the shoots and roots, and it explained the results well. In summary, the optimum concentration of 0.1 mmol L-1 AsA can be implemented to improve the seed germination and seedling growth of alfalfa under salt stress.

show abstract

Leaf mesophyll K+ and Cl− fluxes and reactive oxygen species production predict rice salt tolerance at reproductive stage in greenhouse and field conditions

Cited by 21 publications

References 81 publications

Sodium sequestration confers salinity tolerance in an ancestral wild rice

Sodium sequestration confers salinity tolerance in an ancestral wild rice

Proto Kranz-like leaf traits and cellular ionic regulation are associated with salinity tolerance in a halophytic wild rice

Effects of exogenous ascorbic acid on seed germination and seedling salt-tolerance of alfalfa

Contact Info

Product

Resources

About