Leaf metabolic profiles of two soybean genotypes differentially affect the survival and the digestibility of Anticarsia gemmatalis caterpillars

Gómez, Jenny D.; Pinheiro, Valquiria J.M.; Silva, João Carlos; Romero, Juan Vicente; Meriño‐Cabrera, Yaremis; Coutinho, Flaviane Silva; Lourenção, André Luiz; Serrão, José Éduardo; Vital, Camilo Elber; Fontes, Elizabeth P. B.; Oliveira, M. G. A.; Ramos, Humberto J.O.

doi:10.1016/j.plaphy.2020.07.010

Cited by 19 publications

(17 citation statements)

References 72 publications

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“…Recently, Gómez et al found that compared with the control, a soybean cultivar with M had higher levels of the flavonoids kaempferol-3- O -L-rhamnopyranosyl-glucopyranoside, rutin (quercetin 3- O -rutinoside), quercetin-3,7- O -di-glucoside, quercetin-3- O -rhamnosylglycoside-7- O -glucoside, quercetin-3- O -rhamnopyranosyl-glucopyranoside-rhamnopyranoside, and the isoflavonoids genistein-7- O -diglucosidedimalonyl, genistein-7- O -6- O -malonylglucoside, and daidzein 7- O -glucoside-malonate [ 30 ]. In a follow-up study, Gómez et al identified isorhamnetin glycoconjugates in the resistant genotype, along with increased levels of proteinase inhibitors and flavonoids [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

Metabolomics Differences of Glycine max QTLs Resistant to Soybean Looper

et al. 2021

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Quantitative trait loci (QTLs) E and M are major soybean alleles that confer resistance to leaf-chewing insects, and are particularly effective in combination. Flavonoids and/or isoflavonoids are classes of plant secondary metabolites that previous studies agree are the causative agents of resistance of these QTLs. However, all previous studies have compared soybean genotypes that are of dissimilar genetic backgrounds, leaving it questionable what metabolites are a result of the QTL rather than the genetic background. Here, we conducted a non-targeted mass spectrometry approach without liquid chromatography to identify differences in metabolite levels among QTLs E, M, and both (EM) that were introgressed into the background of the susceptible variety Benning. Our results found that E and M mainly confer low-level, global differences in distinct sets of metabolites. The isoflavonoid daidzein was the only metabolite that demonstrated major increases, specifically in insect-treated M and EM. Interestingly, M confers increased daidzein levels in response to insect, whereas E restores M’s depleted daidzein levels in the absence of insect. Since daidzein levels do not parallel levels of resistance, our data suggest a novel mechanism that the QTLs confer resistance to insects by mediating changes in hundreds of metabolites, which would be difficult for the insect to evolve tolerance. Collective global metabolite differences conferred by E and M might explain the increased resistance of EM.

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Section: Introductionmentioning

confidence: 99%

Metabolomics Differences of Glycine max QTLs Resistant to Soybean Looper

et al. 2021

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“…More recently, bioinsecticides such as Bacillus thuringiensis toxin (Bt) (Badran et al, 2016; Bel et al, 2019), secondary metabolites (phytochemicals) (Gomez et al, 2018, 2020; Lourenço et al, 2018), and protease inhibitors (PI) specific for target pests have gained prominence as alternatives in insect control, minimizing toxic effects on other animals, and the environment (Meriño‐Cabrera et al, 2018; Pilon et al, 2017; da Silva Júnior et al, 2020). PIs are naturally produced as a response strategy for plants, developed throughout the evolutionary process (Cotabarren et al, 2020; Singh et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Intestinal proteases profiling from Anticarsia gemmatalis and their binding to inhibitors

Silva-junior

Cabrera

Barbosa

et al. 2021

Arch Insect Biochem Physiol

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Although the importance of intestinal hydrolases is recognized, there is little information on the intestinal proteome of lepidopterans such as Anticarsia gemmatalis. Thus, we carried out the proteomic analysis of the A. gemmatalis intestine to characterize the proteases by LC/MS. We examined the interactions of proteins identified with protease inhibitors (PI) using molecular docking. We found 54 expressed antigens for intestinal protease, suggesting multiple important isoforms. The hydrolytic arsenal featured allows for a more comprehensive understanding of insect feeding. The docking analysis showed that the soybean PI (SKTI) could bind efficiently with the trypsin sequences and, therefore, insect resistance does not seem to involve changing the sequences of the PI binding site. In addition, a SERPIN was identified and the interaction analysis showed the inhibitor binding site is in contact with the catalytic site of trypsin, possibly acting as a regulator. In addition, this SERPIN and the identified PI sequences can be targets for the control of proteolytic activity in the caterpillar intestine and serve as a support for the rational design of a molecule with greater stability, less prone to cleavage by proteases and viable for the control of insect pests such as A. gemmatalis.

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“…Therefore, attempts to achieve robust nucleic acid isolates are challenging. This increase in plant chemical compound production is thought to be an active self-defence mechanism, resulting from phytoalexin and phytohormone activation; this mechanism can be triggered in several ways, including pathogen infection, pest infestation, or abiotic stress (Afifah et al 2019;Castro-Moretti, Gentzel et al 2020;Gómez et al 2020;Lima et al 2017;Pontes, Ohashi et al 2016). These defensive compounds can become a significant inhibitory factor when seeking to extract high quality nucleic acids in sufficient quantity for genetic assays such as pathogen identification.…”

Section: Introductionmentioning

confidence: 99%

Fungal Basidiomycete Ceratobasidium theobromae DNA obtained directly from cocoa petioles

Junaid

Guest

Purwantara³

2021

Biodiversitas

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Abstract. Junaid M, Purwantara A, Guest G. 2021. Fungal Basidiomycete Ceratobasidium theobromae DNA obtained directly from cocoa petioles. Biodiversitas 22: 2838-2843. Understanding the biology of the fastidious Basidiomycete Ceratobasidium theobromae occupying host-tissue is essential for plant disease management. Direct pathogen DNA extraction from infected plant tissue avoids the need for isolation in artificial media. We report a modified DNA isolation protocol to obtain total plant DNA designed to overcome DNA extraction and isolation problems caused by infected petioles rich in polysaccharides and phenolic substances as a primary source of gummosis. This study examined and compared total plant DNA isolated from petioles high in polysaccharides and polyphenolic compounds using two methods: conventional CTAB lysis buffer and Kits (the standard method), and a new modified CTAB protocol to address these PCR inhibitors. The modified method resulted in higher quality and quantity of C. theobromae crude DNA and amplified PCR product. The modified method produced large quantities of clear, transparent, aqueous DNA-containing lysate (crude DNA) with a clear separation between the upper crude DNA and organic waste layers. Mean DNA absorbance was 1.80, and the lowest DNA yield was 836.6 ng/µl. With the standard method, the blurred, viscous lysate obtained showed signs of gummosis, with poor separation between layers of crude DNA, polysaccharides, protein, and organic waste layers and low yield. Gel electrophoresis indicated poor quality DNA extract. We conclude that this modified method will be valuable for genetic diversity and disease studies in a range of previously challenging plant tissues and their pathogens.

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Leaf metabolic profiles of two soybean genotypes differentially affect the survival and the digestibility of Anticarsia gemmatalis caterpillars

Cited by 19 publications

References 72 publications

Metabolomics Differences of Glycine max QTLs Resistant to Soybean Looper

Metabolomics Differences of Glycine max QTLs Resistant to Soybean Looper

Intestinal proteases profiling from Anticarsia gemmatalis and their binding to inhibitors

Fungal Basidiomycete Ceratobasidium theobromae DNA obtained directly from cocoa petioles

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