Leaf Whorl Inoculation Method for Screening Sugarcane Rust Resistance

Sood, Sushma; Comstock, Jack C.; Glynn, Neil C.

doi:10.1094/pdis-93-12-1335

Cited by 58 publications

(65 citation statements)

References 9 publications

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“…Therefore, evaluation and screening of genotypes for resistance to rusts in the CP program are critical for sustainable sugarcane production in USA and other countries. Although Sood et al (2009) developed a whorl inoculation method to more accurately and efficiently test sugarcane genotype resistance in brown rust (Sood et al, 2009) and orange rust (Zhao et al, 2011), it is still difficult to use the artificial inoculation test in Stage I of the CP program because of a large number (12,000-15,000) of genotypes in this stage due to limited resources (Zhao et al, 2012). Therefore, natural infection has been the primary means of assessing rust resistance in the Stage-I sugarcanes in the CP program.…”

Section: Introductionmentioning

confidence: 99%

Field Evaluation of Sugarcane Orange Rust for First Clonal Stage of the CP Cultivar Development Program

Zhao¹,

Davidson²,

Baltazar³

et al. 2015

American Journal of Agricultural and Biological Sciences

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Consistent development of high-yielding sugarcane (a complex hybrid of Saccharum spp.) cultivars with resistance or tolerance to biotic and abiotic stresses is critical to commercial sugarcane production. Currently, orange rust (caused by Puccinia kuehnii E.J. Butler) is a big challenge for the sugarcane production in Florida, USA. A better understanding of sugarcane genotypic variability in response to orange rust disease will help optimize breeding and selection strategies for disease resistance. Orange rust ratings, scaled from non-infection (0) to severe infection (4) with intervals of 0.5, were recorded from genotypes at the first clonal selection stage (Stage I) of the Canal Point sugarcane breeding and cultivar development program in Florida. Data were collected from all 14,272 and 12,661 genotypes and four replicated reference cultivars, CP 78-1628, CP 80-1743, CP 88-1762 and CP 89-2143, in July-August 2012 and 2013, respectively. Mean rust rating, % of rust infection and rust severity in each family (i.e., progeny of the cross from a female and male) and female parent and their Coefficients of Variation (CV) within and among families (females) were estimated. Results indicated that considerable variation exists in rust tolerance among families or females. The families or females for their progenies with the high susceptibility or resistance to orange rust were identified and ranked. The findings of this study are useful for evaluating sugarcane crosses and parents for rust disease and can help breeders use desirable parents for crossing and improve genotypic resistance to orange rust in the sugarcane breeding programs.

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Section: Introductionmentioning

confidence: 99%

Field Evaluation of Sugarcane Orange Rust for First Clonal Stage of the CP Cultivar Development Program

Zhao¹,

Davidson²,

Baltazar³

et al. 2015

American Journal of Agricultural and Biological Sciences

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“…Screening of CP 05‐1526 for disease reactions was conducted by inoculation testing and/or monitoring for natural infection to smut, leaf scald, brown rust, orange rust, mosaic, Sugarcane yellow leaf virus , eye spot [caused by Bipolaris sacchari (E.J. Butler)], and ratoon stunt in greenhouses and/or under field conditions using the standard methods in the CP program (Comstock et al, 1999; Sood et al, 2009; Glaz et al, 2013). The rating scale for brown and orange rusts consisted of five classes: 0 (resistant), 1 (moderately resistant), 2 (moderately susceptible), 3 (susceptible), and 4 (highly susceptible) determined primarily on the basis of size and number of uredia (Sood et al, 2009; Zhao et al, 2011).…”

Section: Methodsmentioning

confidence: 99%

Registration of ‘CP 05‐1526’ Sugarcane

Zhao¹,

Comstock²,

Glaz³

et al. 2013

J of Plant Registrations

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‘CP 05‐1526’ (Reg. No. CV‐155, PI 667554) sugarcane (a complex hybrid of Saccharum spp.) was developed through cooperative research conducted by the USDA‐ARS, the University of Florida, and the Florida Sugar Cane League, Inc., and released to growers for organic (muck) and sand soils in Florida in October 2012. CP 05‐1526 was selected from the cross CP 98‐1029 × CP 88‐1162 made at Canal Point, FL in December 2002. The female parent (CP 98‐1029) is a sugarcane cultivar released for commercial use in Florida in 2005. The male parent (CP 88‐1162) is an experimental clone of the Canal Point sugarcane breeding and cultivar selection program. CP 05‐1526 was released because of its high cane and sucrose yields and acceptable commercial recoverable sucrose on both muck and sand soils, and its acceptable levels of resistance to brown rust (caused by Puccinia melanocephala H. & P. Sydow), orange rust (caused by Puccinia kuehnii E.J. Butler), leaf scald (caused by Xanthomonas albilineans Ashby, Dowson), Sugarcane mosaic virus strain E (mosaic), ratoon stunt (caused by Leifsonia xyli subsp. xyli Evtsuhenko et al.), and smut (caused by Ustilago scitaminea H. & P. Sydow) in Florida. CP 05‐1526 has an intermediate level of freeze tolerance based on its relative rank of 11 in 22 genotypes tested and analyzed for temporal sucrose deterioration under field conditions.

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“…Brown rust is one of the most devastating diseases for sugarcane production in Florida, Louisiana, and many other production areas around the world [12,[16][17][18]. Using proper parents with resistance to brown rust to make crosses is important in the CP program.…”

Section: Evaluation Of Females Based On Mean Rust Ratings Of Their Prmentioning

confidence: 99%

“…Therefore, evaluation and screening genotypes for rust resistance in the CP program are important for sustainable sugarcane production in the USA and other countries. Although Sood et al [18] developed a whorl inoculation method to more accurately and efficiently test sugarcane genotype resistance in brown rust, it is still difficult to use the artificial inoculation test in the first clonal stage (Stage I) of the CP program because of a large number (12,000-15,000) of genotypes in this stage and because of limited resources [9]. Therefore, natural infection has been the primary means of assessing rust resistance in the Stage-I clones and further artificial inoculation tests for rust resistance are usually used in later stages (Stages II and III) of the CP program [23].…”

Section: Introductionmentioning

confidence: 99%

“…Sugarcane brown rust was first recorded in the United States in 1978. It can cause substantial losses of sugarcane growth, yields, and profits [13][14][15][16][17] and has been responsible for the withdrawal of commercial cultivars in the United States and in most sugarcane growing regions of the world [18]. Although the negative effects of brown rust on sugarcane growth and yield have been reduced by breeding and management practices, the disease is still one of most important concerns in Florida sugarcane production ( Figure 1).…”

Section: Introductionmentioning

confidence: 99%

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Screening for Sugarcane Brown Rust in the First Clonal Stage of the Canal Point Sugarcane Breeding Program

Zhao

Davidson²,

Baltazar³

et al. 2015

Agronomy

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Sugarcane (Saccharum spp.) brown rust (caused by Puccinia melanocephala Syd. & P. Syd.) was first reported in the United States in 1978 and is still one of the great challenges for sugarcane production. A better understanding of sugarcane genotypic variation in response to brown rust will help optimize breeding and selection strategies for disease resistance. Brown rust ratings were scaled from non-infection (0) to severe infection (4) with intervals of 0.5 and routinely recorded for genotypes in the first clonal selection stage of the Canal Point sugarcane breeding program in Florida. Data were collected from 14,272 and 12,661 genotypes and replicated check cultivars in 2012 and 2013, respectively. Mean rust rating, % infection, and severity in each family and progeny of female parent were determined, and their coefficients of variation (CV) within and among families (females) were estimated. Considerable variation exists in rust ratings among families or females. The families and female parents with high susceptibility or resistance to brown rust were identified and ranked. The findings of this study can help scientists to evaluate sugarcane crosses and parents for brown rust disease, to use desirable parents for crossing, and to improve genetic resistance to brown rust in breeding programs. OPEN ACCESSAgronomy 2015, 5 342

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Leaf Whorl Inoculation Method for Screening Sugarcane Rust Resistance

Cited by 58 publications

References 9 publications

Field Evaluation of Sugarcane Orange Rust for First Clonal Stage of the CP Cultivar Development Program

Field Evaluation of Sugarcane Orange Rust for First Clonal Stage of the CP Cultivar Development Program

Registration of ‘CP 05‐1526’ Sugarcane

Screening for Sugarcane Brown Rust in the First Clonal Stage of the Canal Point Sugarcane Breeding Program

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