Abstract-In this work the advantages of GaN HEMTs grown on native GaN substrates over GaN/Si or GaN/Sapphire substrates are investigated, and correlated with epitaxial quality. TEM plane view and cross section analysis of GaN/GaN revealed dislocation density lower than 1 × 10 6 cm −2 , which is at least 3 orders of magnitude lower than the case of GaN/Si or GaN/Sapphire. In the case of GaN/Si, the dislocations did not necessarily originate from the substrate/nucleation layer interface, but the strain relief and isolation buffer stacks were main contributors to the dislocation density. GaN/GaN HEMTs demonstrated superior electrical and thermal performance. GaN/GaN demonstrated 3 orders of magnitude lower off-state leakage, current collapse (Ron increase) after stress bias less than 15% compared to 50% in the case of GaN/Si, and 2% drop of the onstate current due to self-heating in DC operation as compared to 13% and 16% for GaN/Si and GaN/Sapphire respectively. The GaN/Si thermal performance approached GaN/GaN only by substrate removal. Therefore GaN/GaN can allow high on-state current, low off-state leakage current, minimal current collapse, and enhanced thermal dissipation capability at the same time, which can be directly correlated to the absence of high dislocation densities.