Learning and memory in zebrafish larvae

Roberts, Adam C.; Bill, Brent R.; Glanzman, David L.

doi:10.3389/fncir.2013.00126

Cited by 94 publications

(91 citation statements)

References 154 publications

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“…Zebrafish display a number of complex behavioural patterns which are highly comparable to rodents and humans (Roberts et al 2013). Among others, they show fearful, aggressive and exploratory behaviours.…”

Section: Development Of the Zebrafish Neurosystemmentioning

confidence: 99%

Comparability of behavioural assays using zebrafish larvae to assess neurotoxicity

Legradi

Abdellaoui

Pomeren

et al. 2014

Environ Sci Pollut Res

104

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Testing of compounds for neurotoxicity has become increasingly important in recent years. It has been shown that neurological disorders like autism may be related to chemical exposures, which may play a crucial role in the progression of these diseases. Special attention has been be given to the substances causing developmental neurotoxicity as the developing nervous system is more vulnerable to impacts by chemicals than the adult nervous system. The zebrafish (Danio rerio) is a well-established model species in developmental biology and an emerging model in behavioural and neurological studies. Zebrafish larvae display numerous behavioural patterns highly similar to rodents and humans. Their physical characteristics make them well suited for automated high-throughput screening. In the last years, the number of behavioural studies conducted with zebrafish larvae has increased notably. The goal of this review is to provide an overview of behavioural assays commonly used to test substances for developmental neurotoxicity. Literature from 1995 to 2014 was reviewed and focussed on assays performed with zebrafish larvae younger than 7 days post fertilization (dpf). The behavioural tests were scrutinized, and parameters describing the different experimental setups were defined. In the next step, we investigated if differences in the experimental parameters alter the outcome of the test. In order to test the comparability of behavioural assays, we analysed several studies using ethanol, valproate and pentylenetetrazole as model substances. Based on our findings, we provide recommendations which could help improve future behavioural studies performed with zebrafish larvae.

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Section: Development Of the Zebrafish Neurosystemmentioning

confidence: 99%

Comparability of behavioural assays using zebrafish larvae to assess neurotoxicity

Legradi

Abdellaoui

Pomeren

et al. 2014

Environ Sci Pollut Res

104

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“…However, zebrafish, like all teleost fish, exhibit significant and well-documented learning capabilities (Al-Imari & Gerlai, 2008; Colwill, Raymond, Ferreira, & Escudero, 2005; Karnik & Gerlai, 2012; Sison & Gerlai, 2010; Williams, White, & Messer, 2002). Furthermore, the capacity for both nonassociative and associative learning is present even in zebrafish larvae (Aizenberg & Schuman, 2011; Best, Berghmans, Hunt, Clarke, Fleming, Goldsmith, & Roach, 2008; Hinz, Aizenberg, Tushev, & Schuman, 2013; Roberts, Bill, & Glanzman, 2013; Roberts, Reichl, Song, Dearinger, Moridzadeh, Lu, Pearce, Esdin, & Glanzman, 2011; Wolman & Granato, 2011). Recently, long-term memory has been documented in larval zebrafish (Hinz et al, 2013; Wolman, Jain, Liss, & Granato, 2011).…”

Section: Introductionmentioning

confidence: 99%

Long-term habituation of the C-start escape response in zebrafish larvae

Roberts

Pearce

Choe

et al. 2016

Neurobiology of Learning and Memory

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The cellular and molecular basis of long-term memory in vertebrates remains poorly understood. Knowledge regarding long-term memory has been impeded by the enormous complexity of the vertebrate brain, particularly the mammalian brain, as well as by the relative complexity of the behavioral alterations examined in most studies of long-term memory in vertebrates. Here, we demonstrate a long-term form of nonassociative learning—specifically, long-term habituation (LTH)—of a simple reflexive escape response, the C-start, in zebrafish larvae. The C-start is triggered by the activation of one of a pair of giant neurons in the zebrafish’s hindbrain, the Mauthner cells. We show that LTH of the C-start requires the activity of NMDA receptors and involves macromolecular synthesis. We further show that the long-term habituated reflex can by rapidly dishabituated by a brief tactile stimulus. Our results set the stage for rigorous, mechanistic investigations of the long-term memory for habituation of a reflexive behavioral response, one that is mediated by a relatively simple, neurobiologically tractable, neural circuit. Moreover, the demonstration of NMDAR and transcriptionally dependent LTH in a translucent vertebrate organism should facilitate the use of optical recording, and optogenetic manipulation, of neuronal activity to elucidate the cellular basis of a long-term vertebrate memory.

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“…Other behavioural outcomes, as monitored in mammals (autonomic-affective behaviour, based on the organismal self-regulation and emotion; cognitive behaviour patterns, related to attention, memory, evaluation, reasoning, problem solving and decision making), reproductive (behaviour patterns considered to be sex-specific) have not been part of this systematic review. This is because (1) such behavioural patterns are mainly related to juvenile and adult life stages, rather than being manifest in the selected early developmental stages in zebrafish as the model should be compliant with 3R or (2) adequate methods have not yet been developed for these early developmental stages, though recent developments with respect to habituation of larvae (~5 dpf) are reviewed by (Roberts et al, 2013).…”

Section: Background On Behavioral Profiles In Zebrafish In Relation Tmentioning

confidence: 99%

Literature review on in vitro and alternative Developmental Neurotoxicity (DNT) testing methods

Fritsche

Alm

Baumann

et al. 2015

EFSA Supporting Publications

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The goal of this systematic review performed under a contract with EFSA was the evaluation of information on assessment methods in the field of developmental neurotoxicity (DNT). Therefore, a systematic and comprehensive literature search and collection of scientific literature and all other relevant grey literature and website information (in English) from past 20 years until mid of April 2014 on the state of the art of in vivo DNT testing methods including novel and alternative non-mammalian models, in vitro test methods, in silico methods, read across and combination of testing methods in test batteries was performed. This systematic review identified a variety of methods covering early and later stages of neurodevelopment that have the ability to predict DNT of chemicals. Few in vivo models alternative to OECD TG 426 were identified. In general, the available published in vivo data is scattered and heterogeneous, making comparisons across exposure paradigms and compounds very difficult. Thus, to make more useful evaluations, publications with more consistent experimental designs are needed, and more focused in vivo-in vitro comparisons are needed to establish useful effect biomarkers and testing models. From the alternative methods, a testing strategy covering early and later neurodevelopmental stages (from stem cell to zebrafish larvae motor behaviour) can be assembled. For gaining regulatory acceptance, definition of biological application domains of alternative methods by performing e.g. in vitro -in vivo validation is needed. Moreover, protocols for cell-based and zebrafish assays need international standardization. With such standardized protocols, the test battery needs to be tested for its sensitivity and specificity by testing concentration-responses of known DNT positive and negative compounds across the different assays. Such data might then be used either for regulatory decision-making or compound prioritization in favour of a reduction of rodent guideline in vivo testing.

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Learning and memory in zebrafish larvae

Cited by 94 publications

References 154 publications

Comparability of behavioural assays using zebrafish larvae to assess neurotoxicity

Comparability of behavioural assays using zebrafish larvae to assess neurotoxicity

Long-term habituation of the C-start escape response in zebrafish larvae

Literature review on in vitro and alternative Developmental Neurotoxicity (DNT) testing methods

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