“…The energy of biological ammonium conversion to dinitrogen gas approximately occupies ∼21.5%–51.2% of the energy (kWh/kg N) that is used to convert dinitrogen gas to ammonium in the industry (12.1 kWh/kg NH 3 -N). 2 , 3 Besides the required energy, another main drawback is the production of nitrous oxide (N 2 O), which is a potent greenhouse gas and is a 300-fold stronger greenhouse effect than CO 2 . 4 …”