Aiming at the seismic design requirement of "strong column-weak beam" frame structure and combined with test data analysis and computer simulation, the influence of slab bar participation on inelastic response and the yielding-damage mechanism of frame structure have been investigated. Two design schemes that considering the slab bars or neglecting the slab bars were respectively applied to an example frame building to compare the damage situations, which were simulated by the nonlinear time history analysis program SARCF (Seismic Analysis of Reinforced Concrete Frames). The research indicated that slab bars in the tensile zone have a direct enhancing action on the capacity of the frame beam resisting negative moment, which will increase with the augmentation of the monolithic deformation of the beam-slab member. The effect of slab bar participation will develop adequately after the peak ground acceleration acting on the structure reaches the level of design basic acceleration of ground motion. The design method neglecting the slab bars will underestimate the actual flexural strength of the frame beam, which will result in a change of seismic behavior of the structure from "strong column-weak beam" to "strong beam-weak column", and the probability of the change will increase with the augmentation of earthquake action.