Biomass energy can mitigate climate change, revitalize rural economies, and achieve energy independence. Using biomass energy as subject matter content, American agricultural education programs at the secondary school level can prepare future agricultural professionals with science, technology, engineering, and mathematics skills to solve complex issues. Through a state-wide survey (N = 100) in the U.S., this study found agriculture teachers' interest in biomass energy is motivated by economic, environmental, pedagogical, and learning factors. Nine relevant topics were determined as high training needs perceived by teachers. Teacher educators are recommended to incorporate the four factors and nine topics in planning, delivering, and evaluating in-service training programs of biomass energy for agriculture teachers.