The research aims to determine the feasibility and effectiveness of guided inquiry-based worksheets. This type of development research with the Borg & Gall model. Test the effectiveness of LKPD using pre-experimental research. The test subjects were class X students. Data collection techniques included observation, interviews, questionnaires, and test questions. Data analysis technique using descriptive analysis. The results showed that the developed guided inquiry-based LKPD was stated to be very good or very feasible based on the results of validation of material experts, validation of learning design experts, biology teachers, individual group trial results, small group trial results, large group trial results, and improvement students' scientific literacy in the medium category. The results of the effectiveness test using the paired sample test obtained a sig value of 0.00 <0.05 so that the Guided Inquiry-based LKPD is effectively used in the learning process and can increase students' scientific literacy.