The objective was to evaluate the knowledge, attitudes, and practices of the Lebanese population towards cholera and its vaccination. A cross-sectional study was conducted using a self-administered questionnaire among a sample of Lebanese residents. The questionnaire consisted of questions about the participants' demographics, knowledge, attitudes, and practice towards cholera and vaccination. The final sample size was 691 respondents. The proportion of female and male participants was 68.7% and 31.3%, respectively. The results showed that the majority had a good knowledge of cholera, with 97.4% identifying severe diarrhea as the main symptom. Young age, different governorate of residence, and higher education were associated with better knowledge. Most participants had positive attitude, which was correlated with being female and having children under the age of 5 in the same household. Regarding practices, the majority reported engaging in some form of cholera prevention, such as always cooking (90.8%) and washing (88.3%) food. Being older, female, and homemaker was associated with better practice. Most participants expressed good knowledge and a positive attitude towards the vaccine, with 55.5% indicating that they would surely be willing to be vaccinated if freely available. However, 14.2% participants rejected and expressed concerns about the vaccine. Higher cholera knowledge was significantly predictive of vaccination willingness (B = 0.091). In conclusion, this study has provided insights into the knowledge, attitudes, and practices of the Lebanese population towards cholera and its vaccination. The findings can be used to inform the development of public health interventions aimed at improving cholera prevention and control in Lebanon.