Lecithin–chitosan–TPGS nanoparticles as nanocarriers of (−)-epicatechin enhanced its anticancer activity in breast cancer cells

Perez-Ruiz, Adriana Guadalupe; Ganem-Rondero, Adriana; Olivares-Corichi, Ivonne María; García-Sánchez, José Rubén

doi:10.1039/c8ra06327c

Cited by 45 publications

(15 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It should be highlighted that regardless to the presence of lecithin, the mass ratio played an important role in PS as explained in section 3.3.1 where larger PS nanoparticles were attained at low mass ratios and smaller ones were obtained at higher mass ratio. Also, as reported by Perez-Ruiz et al ( 2018 ), accompanied increase in GTE entrapment efficiency contributed in the increased PS. The polydispersity index of almost all formulae was below 0.5 which indicated good stability and homogeneity of nanoparticle dispersion (Manchanda & Sahoo, 2017 ).…”

Section: Resultssupporting

confidence: 72%

Hybrid chitosan-lipid nanoparticles of green tea extract as natural anti-cellulite agent with superior in vivo potency: full synthesis and analysis

2021

View full text Add to dashboard Cite

The aim of this work is to exploit the advantages of chitosan (CS) as a nanocarrier for delivery of anti-cellulite drug, green tea extract (GTE), into subcutaneous adipose tissue. Primarily, analysis of herbal extract was conducted via newly developed and validated UPLC method. Ionic gelation method was adopted in the preparation of nanoparticles where the effect lecithin was investigated resulting in the formation of hybrid lipid-chitosan nanoparticles. Optimal formula showed a particle size of 292.6 ± 8.98 nm, polydispersity index of 0.253 ± 0.02, zeta potential of 41.03 ± 0.503 mV and an entrapment efficiency percent of 68.4 ± 1.88%. Successful interaction between CS, sodium tripolyphosphate (TPP) and lecithin was confirmed by Fourier-transform infrared spectroscopy, differential scanning calorimetry and X-ray diffraction. Morphological examination was done using transmission electron microscope and scanning electron microscope confirmed spherical uniform nature of GTE load CS-TPP nanoparticles. Ex vivo permeation study revealed permeability enhancing activity of the selected optimal formula due to higher GTE deposition in skin in comparison to GTE solution. Moreover in vivo study done on female albino Wistar rats carried out for 21 days proved successful potential anti-cellulite activity upon its application on rats’ skin. Histological examination showed significant reduction of adipocyte perimeter and area and fat layer thickness. Results of the current study demonstrated that the developed GTE-loaded CS-TPP nanoparticle comprised of chitosan and lecithin showed permeability enhancing activity along with the proven lipolytic effect of green tea represent a promising delivery system for anti-cellulite activity.

show abstract

Section: Resultssupporting

confidence: 72%

Hybrid chitosan-lipid nanoparticles of green tea extract as natural anti-cellulite agent with superior in vivo potency: full synthesis and analysis

2021

View full text Add to dashboard Cite

show abstract

“…There are also studies dedicated to investigating EC nanoparticles. Perez-Ruis showed an inhibitory effect of EC nanoparticles on human breast cancer cell lines, which was greater than the effect of free EC [ 53 ].…”

Section: Absorption and Toxicity Of Ec And Txmentioning

confidence: 99%

Mechanisms Modified by (−)-Epicatechin and Taxifolin Relevant for the Treatment of Hypertension and Viral Infection: Knowledge from Preclinical Studies

Bernátová

Líšková

2021

Antioxidants

View full text Add to dashboard Cite

Various studies have shown that certain flavonoids, flavonoid-containing plant extracts, and foods can improve human health. Experimental studies showed that flavonoids have the capacity to alter physiological processes as well as cellular and molecular mechanisms associated with their antioxidant properties. An important function of flavonoids was determined in the cardiovascular system, namely their capacity to lower blood pressure and to improve endothelial function. (−)-Epicatechin and taxifolin are two flavonoids with notable antihypertensive effects and multiple beneficial actions in the cardiovascular system, but they also possess antiviral effects, which may be of particular importance in the ongoing pandemic situation. Thus, this review is focused on the current knowledge of (−)-epicatechin as well as (+)-taxifolin and/or (−)-taxifolin-modified biological action and underlining molecular mechanisms determined in preclinical studies, which are relevant not only to the treatment of hypertension per se but may provide additional antiviral benefits that could be relevant to the treatment of hypertensive subjects with SARS-CoV-2 infection.

show abstract

“…The optimised formulation was dried in SHEL LAB SVAC2-2 vacuum oven (Sheldon Manufacturing Inc., USA) under 35°C and 25 in. Mercury (Hg) before analysis (16). Black alumina was used as a reference.…”

Section: Differential Scanning Calorimetrymentioning

confidence: 99%

“…The use of polymers and amphiphilic molecules has constantly been involved in the delivery of hydrophilic and lipophilic molecules. Lecithin (phosphatidylcholine) is used in various nanocarrier-designed formulations like micelles, liposomes, and lipid nanoparticles (15)(16). Lecithin is not only used due to its safe and biocompatibility properties, but also because it is the solubilising carrier for poorly water-soluble drugs (17).…”

Section: Introductionmentioning

confidence: 99%

PEGylated Lipid Polymeric Nanoparticle–Encapsulated Acyclovir for In Vitro Controlled Release and Ex Vivo Gut Sac Permeation

et al. 2020

View full text Add to dashboard Cite

Currently, pharmaceutical research is directed wide range for developing new drugs for oral administration to target disease. Acyclovir formulation is having common issues of short half-life and poor permeability, causing messy treatment which results in patient incompliance. The present study formulates a lipid polymeric hybrid nanoparticles for antiviral acyclovir (ACV) agent with Phospholipon® 90G (lecithin), chitosan, and polyethylene glycol (PEG) to improve controlled release of the drugs. The study focused on the encapsulation of the ACV in lipid polymeric particle and their sustained delivery. The formulation developed for the self-assembly of chitosan and lecithin to form a shell encapsulating acyclovir, followed by PEGylation. Optimisation was performed via Box-Behnken Design (BBD), forming nanoparticles with size of 187.7 ± 3.75 nm, 83.81 ± 1.93% drug-entrapped efficiency (EE), and + 37.7 ± 1.16 mV zeta potential. Scanning electron microscopy and transmission electron microscopy images displayed spherical nanoparticles formation. Encapsulation of ACV and complexity with other physical parameters are confirmed through analysis using Fourier transform infrared spectroscopy, differential scanning calorimetry, and X-ray diffraction. Nanoparticle produced was capable of achieving 24-h sustained release in vitro on gastric and intestinal environments. Ex vivo study proved the improvement of acyclovir's apparent permeability from 2 × 10 −6 to 6.46 × 10 −6 cm s −1. Acyclovir new formulation was achieved to be stable up to 60 days for controlled release of the drugs.

show abstract

Lecithin–chitosan–TPGS nanoparticles as nanocarriers of (−)-epicatechin enhanced its anticancer activity in breast cancer cells

Abstract: Natural compounds such as (−)-epicatechin show a variety of biological properties including anticancer activity.

Cited by 45 publications

References 43 publications

Hybrid chitosan-lipid nanoparticles of green tea extract as natural anti-cellulite agent with superior in vivo potency: full synthesis and analysis

Hybrid chitosan-lipid nanoparticles of green tea extract as natural anti-cellulite agent with superior in vivo potency: full synthesis and analysis

Mechanisms Modified by (−)-Epicatechin and Taxifolin Relevant for the Treatment of Hypertension and Viral Infection: Knowledge from Preclinical Studies

PEGylated Lipid Polymeric Nanoparticle–Encapsulated Acyclovir for In Vitro Controlled Release and Ex Vivo Gut Sac Permeation

Contact Info

Product

Resources

About