LecRKIII.1 and LecRKIII.2 formed homodimers to play physiological functions in Arabidopsis thaliana

Abstract

Receptor-like kinases (RLKs), an important family that plant cell sense and transmit extracellular signals, are fundamental to plant cell life and play critical roles in various biological processes, such as response to abiotic stress, development and hormonal response. Among RLKs, the lectin receptor-like kinases (LecRLKs) possess a characteristic extracellular carbohydrate-binding lectin domain and play important roles in plant immune, stress and hormone responses. However, little is known about the genes LecRKIII.1 and LecRKIII.2 of LecRLK family up to now. Here, we founded that LecRKIII.1 and LecRKIII.2 were localized in plasma membrane and played the physiological roles by forming a homodimer. Real-time fluorescence quantitative PCR analysis of the expression level of LecRKIII.1 and LecRKIII.2 in response to exogenous ABA, MeJA, SA, NaCl, mannitol and dehydration suggested that LecRKIII.1 and LecRKIII.2 may play important roles in response to various stresses. It will lay a good foundation for further studies on physiological functions of LecRKIII.1 and LecRKIII.2.

LORE receptor homomerization is required for 3-hydroxydecanoic acid-induced immune signaling and determines the natural variation of immunosensitivity within the Arabidopsis genus

“…The cell death correlated with transgene expression levels and was dependent on kinase activity, but not on extracellular lectin domains. Homomerization was not shown for AtLecRK-IX.1 and AtLecRK-IX.2, yet is also shown for other AtLecRKs (Guo et al, 2018). Taken together, this suggests that homomerizing RLKs are in particular prone to spontaneous auto-activation upon strong overexpression, resulting in cell death.…”

“…AtLecRK-IX.1 and AtLecRK-IX.2 exhibit cell death upon overexpression in A. thaliana and N. benthamiana (Wang et al, 2015). Although AtLecRK-IX.1/2 homomerization was not reported, it is shown for other AtLecRKs (Guo et al, 2018). In A. thaliana, overexpression of the co-receptor AtBAK1, which participates in numerous signaling pathways, results in leaf necrosis and impaired plant development, presumably due to spontaneous activation of multiple receptor complexes (Domínguez-Ferreras et al, 2015).…”

LORE receptor homomerization is required for 3‐hydroxydecanoic acid‐induced immune signaling and determines the natural variation of immunosensitivity within the Arabidopsis genus

“…At LecRK‐IX.1 and At LecRK‐IX.2 exhibit cell death upon overexpression in A. thaliana and N. benthamiana (Wang et al ., 2015). Although At LecRK‐IX.1/2 homomerization was not reported, it is shown for other At LecRKs (Guo et al ., 2018). In A. thaliana , overexpression of the co‐receptor At BAK1, which participates in numerous signaling pathways, results in leaf necrosis and impaired plant development, presumably due to spontaneous activation of multiple receptor complexes (Domínguez‐Ferreras et al ., 2015).…”