Lectin-binding Capacity of Glycoconjugates in Escherichia coli 09:K103:NM, 987P+ST+-Infected Porcine Lower Small Intestines.

Abstract

ABSTRACT. Composition of glycoconjugates were investigated in Escherichia coli 09:K103:NM, 987P+ ST + -infected lower small intestines of 1-week-old pigs by the use of twenty one biotinylated-labelled lectins with avidin-biotin-peroxidase complex method. Piglets with experimental group were inoculated by feeding 5 ml of culture inoculum (5 × 10 9 colony-forming units/ml) with 15 ml of milk replacer. At the onset of diarrhea, experimental piglets and time-matched control piglets were euthanatized using electrocution, necropsied, and tested by lectin histochemistry. As compared with control, staining intensity of seven lectins altered in ileal villus brush border and goblet cells of pigs inoculated with the pathogen.-KEY WORDS: Escherichia coli, histochemistry, lectin.Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrhea and death in preweaned pigs. The organism has 2 types of virulence factors: fimbrial adhesions and enterotoxins. Of these fimbrial adhesions, 987P is the predominant adherence factor in piglets up to 2 weeks old [9]. Age resistant to 987P is well-understood and piglets older than three weeks appear to be resistant to infection with the strains of E. coli that express the 987P fimbriae [8]. ETEC possess fimbriae that bind to receptors on the brush border of intestinal epithelial cells. These receptors are carbohydrate moieties (often D-galactose, Nacetylglucosamine, N-acetylgalactosamine or Nacetylneuraminic acid) of glycoproteins, glycolipids, proteoglycans or mucins [4,7].Lectin was used to investigate interaction between 987P and the receptors in the intestinal surface [3,4]. Glycoconjugates of intestinal mucins prevent attachment of pathogenic bacteria by modulating their adherence to the intestinal enterocytes [10]. However, little is known about the glycoconjugate composition of intestines in response to E. coli infection.The objective of the present study was to examine whether the alteration of glycoconjugate in lower small intestine with E. coli bearing 987P infection with lectin histochemistry. To determine the specific changes in intestinal glycoconjugates, the lectin-binding capacity to 21 different sugars residues was investigated in the ileum of the pigs experimentally inoculated with the pathogen.Porcine enterotoxigenic E. coli 09:K103:NM, 987P + ST + were used in this study [12]. E. coli 09:K103:NM were tested for 987P fimbriae by slide agglutination with anti-987P serum before use [13]. Thirty two 1-week-old Large White-Landrace crossbred piglets purchased from a commercial swine farm were randomly assigned to experimental and control group. Each group consisted of 16, 1-week-old conventional piglet, respectively. Piglets with experimental group were inoculated by feeding 5 ml of culture inoculum (5 × 10 9 colony-forming units/ml) with 15 ml of milk replacer. Piglets with control group were administered 15 ml of milk replacer only. Piglets were examined 3 times per day post-inoculation (PI) for clinical signs.The experimental piglets and time-matched control piglets ...

Glycoconjugate histochemistry in the small and large intestine of normal and Solanum glaucophyllum-intoxicated rabbits

“…Lectin binding pattern of the intestinal epithelium varies with the species, the anatomical section considered, the period of life (Pang et al, 1987;Falk et al, 1994;Chae and Lee, 1995) and under physiological and pathological conditions. Changes in the intestine of pigs and rats under bacterial infection or parasitosis (Sohn and Chae, 2000;Yamauchi et al, 2006;Thomsen et al, 2006), in young rabbits mucoid enteropathy (Itagaki et al, 1994) and enterotoxemia (Jelinek, 1996) are only some examples. In addition, it has been reported that modifications on nutritional factors and diverse hormonal stimuli may be involved in the glycosylation process (Biol et al, 1992;Sharma et al, 1995;Biol-N'garagba et al, 2002;Biol-N'garagba and Louisot, 2003).…”

Increased fucosyl glycoconjugate by Mycoplasma hyopneumoniae enhances adherences of Pasteurella multocida type A in the ciliated epithelial cells of the respiratory tract

“…Both pretreatments prevented staining. The small intestine tissues from Escherichia coli -infected pigs were used as positive control for lectin histochemistry of UEA-I [ 16 ]. The porcine ileal Peyer’s patches tissues were used as negative control for lectin histochemistry of UEA-I [ 17 ].…”

Protection of Suckling Neonatal Pigs Against Infection with an Enterotoxigenic Escherichia coli Expressing 987P Fimbriae by the Administration of a Bacterial Competitive Exclusion Culture

“…E. coli expressing the 987P adhesin have been found to infect and cause disease in neonatal pigs and not pigs of \ 3 weeks of age (Hall, 1989 (8)). Attachment to the villous epithelium of the brush border lining the lumen of the gut is necessary for ETEC to cause disease (9,10). Attachment is mediated by the 987P mbriae and the corresponding receptor on the brush border of the neonatal host (5).…”