Lectin Binding in Bone Matrix of Adult Rats with Special Reference to Cement Lines.

Kagayama, Manabu; Sasano, Yasuyuki; Akita, Hirotoshi

doi:10.1620/tjem.170.81

Cited by 6 publications

(6 citation statements)

References 22 publications

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“…, osteopontin and other bone sialoproteins). In addition, previous evidence has shown that large amount of sugar residues are present in cement lines [51–53]. Thus, we postulate that at the end of bone resorption osteoclasts may recruit a large amount of carbohydrates at cement lines [51].…”

Section: Discussionmentioning

confidence: 79%

In situ accumulation of advanced glycation endproducts (AGEs) in bone matrix and its correlation with osteoclastic bone resorption

Dong¹,

Qin²,

Xu³

et al. 2011

Bone

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Advanced glycation end products (AGEs) have been observed to accumulate in bone with increasing age and may impose effects on bone resorption activities. However, the underlying mechanism of AGEs accumulation in bone is still poorly understood. In this study, human cortical bone specimens from young (31±6 years old), middle-aged (51±3 years old) and elderly (76±4 years old) groups were examined to determine the spatial-temporal distribution of AGEs in bone matrix and its effect on bone resorption activities by directly culturing osteoclastic cells on bone slices. The results of this study indicated that the fluorescence intensity (excitation wave length 360 nm and emission wave length 470±40 nm) could be used to estimate the relative distribution of AGEs in bone (pentosidine as its marker) under an epifluorescence microscope. Using the fluorescence intensity as the relative measure of AGEs concentration, it was found that the concentration of AGEs varied with biological tissue ages, showing the greatest amount in the interstitial tissue, followed by the old osteons, and the least amount in newly formed osteons. In addition, AGEs accumulation was found to be dependent on donor ages, suggesting that the younger the donor the less AGEs were accumulated in the tissue. Most interestingly, AGEs accumulation appeared to initiate from the region of cement lines, and spread diffusively to the other parts as the tissue aged. Finally, it was observed that the bone resorption activities of osteoclasts were positively correlated with the in situ concentration of AGEs and such an effect was enhanced with increasing donor age. These findings may help elucidate the mechanism of AGEs accumulation in bone and its association with bone remodeling process.

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Section: Discussionmentioning

confidence: 79%

In situ accumulation of advanced glycation endproducts (AGEs) in bone matrix and its correlation with osteoclastic bone resorption

Dong¹,

Qin²,

Xu³

et al. 2011

Bone

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“…The resorption pits on bone substrate in vitro can thus be visualized using the WGA-lectin, which is evidence of specific binding with glycan epitopes located at the resorption lacunae (Selander et al 1994 ). An in vivo study by Kagayama et al ( 1993 ) showed that the lectins Limax flavus (LFA), Maclura pomifera (MPA), WGA and ConA differentially labeled cellular or extracellular components of adult rat bone tissue. However, although animal bone material has been characterized by lectin staining, these results cannot be extrapolated to the situation in human bone, as glycosylation is known to be species-specific.…”

Section: Introductionmentioning

confidence: 99%

Osteoclasts secrete osteopontin into resorption lacunae during bone resorption

Luukkonen

Hilli

Nakamura

et al. 2019

Histochem Cell Biol

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Osteopontin (OPN) is a non-collagenous extracellular sialylated glycoprotein located in bone. It is believed to be one of the key components in osteoclast attachment to bone during resorption. In this study, we characterized OPN and other glycoproteins found in the resorption lacunae to confirm the role of osteoclasts in OPN secretion using electron microscopy and mass spectrometry. Additionally, we examined the glycan epitopes of resorption pits and the effects of different glycan epitopes on the differentiation and function of osteoclasts. Osteoarthritic femoral heads were examined by immunohistochemistry to reveal the presence of OPN in areas of increased bone metabolism in vivo. Our results demonstrate that human osteoclasts secrete OPN into resorption lacunae on native human bone and on carbonated hydroxyapatite devoid of natural OPN. OPN is associated with an elevated bone turnover in osteoarthritic bone under experimental conditions. Our data further confirm that osteoclasts secrete OPN into the resorption pit where it may function as a chemokine for subsequent bone formation. We show that α2,3- and α2,6-linked sialic acids have a role in the process of osteoclast differentiation. OPN is one of the proteins that has both of the above sialic residues, hence we propose that de-sialylation can effect osteoclast differentiation in bone. Electronic supplementary material The online version of this article (10.1007/s00418-019-01770-y) contains supplementary material, which is available to authorized users.

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“…In a given bone cortex, all of these functions have been considered to be influenced by the relative degree of mineralization and differences in the composition of cement lines, and their associated physical interfaces, with respect to immediately surrounding bone. Experimental data showing that cement line regions are enriched with noncollagenous proteins (e.g., osteocalcin, osteopontin, and bone sialoprotein) and other biochemical factors also suggest that, in addition to having important local adhesive and modulus mismatch functions, these narrow seams may also contain molecules that promote osteoclast‐osteoblast coupling during bone remodeling and thereby help to maintain bone homeostasis and biomechanical integrity (e.g., influencing microdamage propagation and arrest, and localization for their repair) (Frasca et al, 1981b; Mundy et al, 1982; Baron et al, 1984; Parfitt, 1984; Hauschka et al, 1986; Ingram et al, 1993; Kagayama et al, 1993; McKee and Nanci, 1995, 1996a; Hosseini et al, 2000; Everts et al, 2002; Sit et al, 2003).…”

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confidence: 99%

Cement lines of secondary osteons in human bone are not mineral‐deficient: New data in a historical perspective

Holmes²,

et al. 2005

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Using qualitative backscattered electron (BSE) imaging and quantitative energy dispersive X-ray (EDX) spectroscopy, some investigators have concluded that cement (reversal) lines located at the periphery of secondary osteons are poorly mineralized viscous interfaces with respect to surrounding bone. This conclusion contradicts historical observations of apparent highly mineralized (or collagen-deficient) cement lines in microradiographs. Such conclusions, however, may stem from unrecognized artifacts that can occur during scanning electron microscopy. These include specimen degradation due to high-energy beams and the sampling of electron interaction volumes that extend beyond target locations during EDX analysis. This study used quantitative BSE imaging and EDX analysis, each with relatively lower-energy beams, to test the hypothesis that cement lines are poorly mineralized. Undemineralized adult human femoral diaphyses (n ϭ 8) and radial diaphyses (n ϭ 5) were sectioned transversely, embedded in polymethyl methacrylate, and imaged in a scanning electron microscope for BSE and EDX analyses. Unembedded samples were also evaluated. Additional thin embedded samples were stained and evaluated with light microscopy and correlated BSE imaging. BSE analyses showed the consistent presence of a bright line (higher atomic number) coincident with the classical location and description of the cement line. This may represent relative hypermineralization or, alternatively, collagen deficiency with respect to surrounding bone. EDX analyses of cement lines showed either higher Ca content or equivalent Ca content when compared to distant osteonal and interstitial bone. These data reject the hypothesis that cement lines of secondary osteons are poorly mineralized.

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Lectin Binding in Bone Matrix of Adult Rats with Special Reference to Cement Lines.

Cited by 6 publications

References 22 publications

In situ accumulation of advanced glycation endproducts (AGEs) in bone matrix and its correlation with osteoclastic bone resorption

In situ accumulation of advanced glycation endproducts (AGEs) in bone matrix and its correlation with osteoclastic bone resorption

Osteoclasts secrete osteopontin into resorption lacunae during bone resorption

Cement lines of secondary osteons in human bone are not mineral‐deficient: New data in a historical perspective

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