Lectin Binding in Human Breast Cancer: Clinical and Pathologic Correlations with Fluorescein-Conjugated Peanut, Wheat Germ and Concanavalin A Binding

Dansey, Roger; Murray, J. G.; Ninin, Daniel; Wr, Bezwoda

doi:10.1159/000226627

Cited by 12 publications

(8 citation statements)

References 9 publications

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“…The resultant CIN ratios for PNA showed no correlation with the ER/PR content of the breast-tumor tissues. This is in contrast to the studies of Bocker et al (1984) and Dansey et al (1988), in which T-antigen expression was reported to posi-tively correlate with the expression of ER in breast cancer. These discrepancies may be due to differences in reagents or methods of analysis used in these studies.…”

Section: Discussioncontrasting

confidence: 99%

Quantitative dot blot analyses of blood‐group‐related antigens in paired normal and malignant human breast tissues

Ura

Dion

Williams

et al. 1992

Intl Journal of Cancer

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Membranes were prepared from 31 breast-cancer specimens and adjacent mammary tissues, dot-blotted to nitrocellulose paper, and reacted with monoclonal antibodies (MAbs) (A, B, Lewis a, Lewis b, sialylated Lewis a, Lewis x, and Lewis y) and lectins (Ulex europaeus, peanut agglutinin) having various blood-group specificities. The expression of epithelial membrane antigen was assayed with MAb MA5. The ratio of breast-cancer to normal mammary membrane preparations (C/N ratios) of these reagents was measured by densitometric scanning. We observed a decrease in the levels of A, B, Lewis a, Lewis b, sialylated Lewis a, and Lewis y antigens and an increase of Lewis x, T, and MA5-reactive determinants in breast cancers. The incidence of incompatible A, as well as A and B, antigens was demonstrated for 2 patients of blood group B and O respectively. When the receptor content was plotted against the C/N ratio of these various reagents, a significant inverse relationship between the C/N ratio of Lewis x antigen and estrogen (ER) and progesterone receptor (PR) content was observed in breast cancers. The mean C/N ratio of Lewis x antigen was significantly higher in the ER-negative/PR-negative (ER-/PR-; 2.33 +/- 1.17), as compared with the ER-positive/PR-positive (ER+/PR+; 0.97 +/- 0.80). According to these observations, Lewis x antigen expression may be influenced by hormonal stimuli such as estrogen and progesterone.

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Section: Discussioncontrasting

confidence: 99%

Quantitative dot blot analyses of blood‐group‐related antigens in paired normal and malignant human breast tissues

Ura

Dion

Williams

et al. 1992

Intl Journal of Cancer

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“…Various lectins with specific carbohydrate binding properties have been used to identify specific cell surface carbohydrate compositions in normal or neoplastic cells (Dansey et al, 1988;Langkilde et al, 1989bLangkilde et al, , 1989cAoki et al, 1990;Hohenberger et al, 1990;Kakeji et al, 1994;Schumacher et al, 1994;Takahashi et al, 1994;Mody et al, 1995). WGA has been shown to react with cell membrane glycoproteins in a wide variety of neoplastic tissues (Willmott and Simpson, 1983;Walker, 1984;Kellokumpu et al, 1986;Dansey et al, 1988;Langkilde et al, 1989a;Bresalier et al, 1990;Welch et al, 1990;Mody et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

“…WGA has been shown to react with cell membrane glycoproteins in a wide variety of neoplastic tissues (Willmott and Simpson, 1983;Walker, 1984;Kellokumpu et al, 1986;Dansey et al, 1988;Langkilde et al, 1989a;Bresalier et al, 1990;Welch et al, 1990;Mody et al, 1995). When WGA was used to generate lectin-resistant clones of cultured cancer cells, most resulting clones showed a remarkable loss of metastatic capabilities (Stanley et al, 1980;Kerbel et al, 1982;Ishikawa et al, 1988;Ishikawa and Kerbel, 1989;Kim et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

Wheatgerm agglutinin-mediated toxicity in pancreatic cancer cells

et al. 1999

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Summary Lectin binding specificities for carbohydrate allow phenotypic and functional characterization of membrane-associated glycoproteins expressed on cancer cells. This analysis examined wheatgerm agglutinin binding to pancreatic cancer cells in vitro and the resulting toxicity. Membrane preparations of nine human pancreatic carcinoma cell lines were studied for lectin binding using wheatgerm agglutinin (WGA), concanavalin A (ConA) and phytohaemagglutinin-L (PHA-L) in a lectin blot analysis. Cell proliferation in vitro was measured by thymidine incorporation in the absence or presence of lectins at various concentrations. Sialic acid binding lectins or succinyl-WGA (succWGA) served as controls. WGA toxicity was tested after swainsonine or neuraminidase pretreatment. Binding and uptake of fluorescein-labelled lectins was studied under fluorescence microscopy. All pancreatic cell lines displayed high WGA membrane binding, primarily to sialic acid residues. Other lectins were bound with weak to moderate intensity only. Lectin toxicity corresponded to membrane binding intensity, and was profound in case of WGA (ID 50 at 2.5-5 µg ml -1 ). WGA exposure induced chromatin condensation, nuclear fragmentation and DNA release consistent with apoptosis. Important steps for WGA toxicity included binding to sialic acid on swainsonine-sensitive carbohydrate and lectin internalization. There was rapid cellular uptake and subsequent nuclear relocalization of WGA. In contradistinction to the other lectins studied, WGA proved highly toxic to human pancreatic carcinoma cells in vitro. WGA binding to sialic acid residues of N-linked carbohydrate, cellular uptake and subsequent affinity to N-acetyl glucosamine appear to be necessary steps. Further analysis of this mechanism of profound toxicity may provide insight relevant to the treatment of pancreatic cancer.

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“…A similar selection can be operative with respect to naturally occurring immunoglobulin fractions, among them one with selectivity to 9-0-acetylated sialic acid (Neu5,9-Ac 2 ) [28,29]. Due to the suggested relevance of concanavalin A (Con A) reactivity for metastasis formation and survival from a cell model and histochemical analysis [26,30,31], this plant lectin was also part of the glycohistochemical panel of this study. Binding of this lectin was compared with that of the collectin to infer differences of the two lectins with identical monosaccharide specificity.…”

Section: Introductionmentioning

confidence: 99%

Pulmonary metastases of breast carcinomas: Ligandohistochemical, nuclear, and structural analysis of primary and metastatic tumors with emphasis on period of occurrence of metastases and survival

Kayser¹,

Biechele²,

Kayser³

et al. 1998

J. Surg. Oncol.

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Background and Objectives Pulmonary metastases of breast carcinomas have a high frequency and are often subject to surgical intervention. To contribute to advances in the knowledge about morphometric and biochemical parameters of primary tumors and their metastatic lesions, analysis of syntactic structure and thermodymanic aspects as well as of expression of distinct glycohistochemical features with respect to period of metastasis occurrence and patient survival is desirable. Methods Clinical history, surgical findings, histopathological reports, survival of the patients with a maximum follow‐up of 15 years, and paraffin blocks of 32 breast carcinoma specimens and their pulmonary metastases were examined. Only potentially curative resections of both the breast carcinoma and their metastases have been included for anlysis. The following markers were applied: neoglycoconjugates with histoblood group A‐ and H‐trisaccharides, lactose, α‐N‐acetyl‐D‐galactosamine and the Forssman disaccharide, a polyclonal immunoglobulin G fraction from human serum with specificity for 9‐0‐acetylated sialic acid, which is a tumor marker for melanomas, the serum lectins serum amyloid P component and mannan‐binding lectin, the mannose‐specific plant lectin concanavalin A, and monoclonal antibodies specific for estrogen and progesterone receptors, respectively. In addition, measurements of the integrated optical density (IOD) and tissue structure were performed. Results The frequency of expression of hormone receptors and expression of binding capacities to most of the applied probes was similar between the primary and metastatic tumors; however, it varied markedly between different patients. For the IOD parameters, a close association between the primary tumors and their metastases was seen, especially a rather low S‐phase‐related tumor cell fraction and a high percentage of tumor cells with an IOD >5C. The mean time for the development of intrapulmonary metastases measured 43 months. It was considerably longer in progesterone receptor‐negative tumors (49.7 months) and those with a lack of expression of sites with specificity for the Forssman disaccharide (48.7 months). The survival was positively correlated with the presence of binding capacity of histoblood group A‐trisaccharide and certain structural parameters, especially the structural entropy and its current. The presence of estrogen and progesterone receptors was not associated with the total survival at a statistically significant level. Conclusions Histochemical features between the primary breast carcinoma and their intrapulmonary metastases can evidently vary. Analysis of the hormone receptor status in metastatic lesions seems to be useful for diagnostic purposes only in rare cases, i.e., distinguishing metastases from primary lung carcinoma. Nonetheless, the survival of patients with metastasizing breast carcinoma is associated with features of the primary tumors, especially the detection of binding capacities for the Forssman disaccharide and the histoblood group A‐...

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Lectin Binding in Human Breast Cancer: Clinical and Pathologic Correlations with Fluorescein-Conjugated Peanut, Wheat Germ and Concanavalin A Binding

Cited by 12 publications

References 9 publications

Quantitative dot blot analyses of blood‐group‐related antigens in paired normal and malignant human breast tissues

Quantitative dot blot analyses of blood‐group‐related antigens in paired normal and malignant human breast tissues

Wheatgerm agglutinin-mediated toxicity in pancreatic cancer cells

Pulmonary metastases of breast carcinomas: Ligandohistochemical, nuclear, and structural analysis of primary and metastatic tumors with emphasis on period of occurrence of metastases and survival

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