Lectin‐binding pattern of the senegalese sole Solea senegalensis oogenesis

Abstract

The glycoconjugate pattern of developing ovarian follicles in wild and cultured Senegalese sole Solea senegalensis was investigated by means of lectin histochemistry. Ovaries from cultured fish contained oocytes up to the late vitellogenic stage, whereas they reached the hydration stage in wild specimens. The follicular cells bound MAL II, SBA, HPA, DBA, Con A, KOH-sialidase (K-s)-WGA, GSA I-B(4) in the late vitellogenic stage, and in wild fish also SNA and K-s-PNA, whereas in the hydration stage SBA, HPA, DBA, and GSA I-B(4) only. The zona radiata reacted with SBA, HPA, DBA, Con A, and GSA I B(4) in the late vitellogenic stage and in cultured fish also with UEA I, whereas in the hydration stage it stained with SBA only. The cortical alveoli bound SBA, HPA, RCA(120) during the late vitellogenic stage, also SNA, PNA, K-s-PNA, GSA I-B(4) in cultured fish, DBA, and K-s-WGA in wild ones which stained with SBA, HPA, and GSA I-B(4) in the hydrated stage. The yolk reacted with Con A in the late vitellogenic oocytes, and also with MAL II, SNA, K-s-PNA, SBA, HPA, K-s-WGA, GSA I-B(4), UEA I in the hydrated ones. From perinucleolus to late vitellogenic stages, the oocyte nucleoplasm bound Con A, GSA I-B(4), GSA II, UEA I, and in wild fish also MAL II, SNA, LTA but only GSA I-B(4) reactivity in the early maturation stage. These findings demonstrate that the glycan pattern of fish ovarian follicles changes during the maturative stages and that it is affected by culture-rearing conditions.

Glycobiology of Reproductive Processes in Marine Animals: The State of the Art

“…The glycoconjugate pattern is specie specific, being cortical alveoli rich in α- N -acetyl- D -glucosamine (α-GlcNAc), sialic acids, α- N -Acetyl- D -galactosamine (α-GalNAc), α- or β-GalNAc, β- D -Galactosyl-(1-3)- N -acetyl- D -galactosamine (β- D -Gal(1-3)-GalNAc) in swordfish [8]; GalNAc and α-galactose (αGal) in flatfish [19]; GlcNAc, GalNAc, Gal and sialic acid in protogynous teleost Epinephelus marginatus [20] and GlcNAc, GalNAc and sialic acid in bluefin tuna [7]. …”

“…Zona radiate of dusky grouper Epinephelus marginatus is characterized by a higher content of GlcNAc and sialic acid [20] as in swordfish zona radiate in which α- L -Fuc residues are also detected [8]. During oocyte development, the glycan pattern changes and this may reflect the different activity of zona radiate during different phases of oogenesis [19]. …”

Comparative morphology of the oocyte surface and early development in four characiformes from the São Francisco River, Brazil

“…In fish and insect eggs, a lectin-binding glycoprotein bound tightly to the micropylar region has a role as sperm guidance toward the micropylar canal (Yanagimachi, et al, 2013). After egg activation, glycoconjugates from the cortical alveoli (CA) are released into the perivitelline space, contributing to chorion hardening, bacterial agglutination, and block to polyspermy (Motta et al, 2005;Accogli, et al, 2012). Additionally, adhesive structures associated with the zona radiata have an essential role in the egg attachment to the substratum (Rizzo et al, 2002).…”

“…Additionally, adhesive structures associated with the zona radiata have an essential role in the egg attachment to the substratum (Rizzo et al, 2002). Since lectins have specific binding affinities for terminal sugar residues (Lis and Sharon, 1998), then lectinbinding pattern can provide suitable knowledge on the glycidic molecules distribution in fish oocytes (Ortiz-Delgado et al, 2008;Accogli, et al, 2012).…”

Differential expression and localization of glycosidic residues in in vitro- and in vivo-matured cumulus-oocyte complexes in equine and porcine species

“…These amino‐acidic residues can also link fucose (Fuc), glucose (Glc), mannose (Man), and N ‐acetylglucosamine (GlcNAc) to form other classes of O ‐linked glycans (reviewed in Lowe and Marth, ). Histochemical techniques using lectins have been widely employed to characterize the oligosaccharide pattern of the glycoproteins present in the ovarian follicles of lower vertebrates (Fang and Welsch, ; Prisco et al, ; Farias et al, ; Rodler, ; Scillitani et al, ; Accogli et al, ) as well as of several mammalian species (see Desantis et al, ). In mammals, lectin histochemistry has been also applied to investigate the glycan pattern in isolated antral oocytes of humans (Talevi et al, ; Jiménez‐Movilla et al, ), cow (Verini‐Supplizi et al, ; de Paz et al, ; Velàsquez et al, 2007), pig (Parillo et al, ; Pastor et al, ), and equine (Desantis et al, ), as well as in the postovulatory oocytes from mouse (Avilés et al, , ), rat (Avilés et al, ) and hamster (Avilés et al, ; El‐Mestra and Kan, 2001).…”