Lectin Binding Sites in Marine Amoebae

Rogerson, Andrew; Polne‐Fuller, Miriam; Gibor, Aharon

doi:10.1016/s0003-9365(11)80044-8

Archiv für Protistenkunde

1992

DOI: 10.1016/s0003-9365(11)80044-8

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Lectin Binding Sites in Marine Amoebae

Andrew Rogerson

Miriam Polne‐Fuller

Aharon Gibor

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Cited by 3 publications

(2 citation statements)

References 22 publications

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“…It has been suggested that psychrophilic amoebae may optimize metabolic functions for existence at low environmental temperatures (Mayes et al 1997). It appears that some of these optimizations may occur within cosmopolitan genera, such as Neoparamoeba , Platyamoeba , and Vannella (Mayes et al 1997; Rogerson, Polne‐Fuller, and Gibor 1992). The Antarctic marine environment also appears to be a source for novel amoebae, such as V. antarctica .…”

mentioning

confidence: 99%

A Description of Seven Antarctic Marine Gymnamoebae Including a New Subspecies, Two New Species and a New Genus: Neoparamoeba aestuarina antarctica n. subsp., Platyamoeba oblongata n. sp., Platyamoeba contorta n. sp. and Vermistella antarctica n. gen. n. sp.

Moran

Anderson

Dennett

et al. 2007

J Eukaryotic Microbiology

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Seven marine gymnamoebae were isolated from different environments of seawater, slush (pack ice meltwater), and sediment in the Ross Sea area of Antarctica. All amoebae were isolated and maintained at temperatures below 4 degrees C. Growth, rate of locomotion, and general morphology were observed at an environmentally appropriate temperature (1 degrees C) and at room temperature (approximately 25 degrees C). Molecular (srDNA sequences) and microscopical techniques were used to identify the gymnamoebae and establish their phylogenetic affinities. Three isolates (S-131-2, SL-200, and W4-3) were assigned to a psychrophilic subspecies of Neoparamoeba aestuarina, N. aestuarina antarctica n. subsp., one isolate (S-205) was assigned to a new species of Platyamoeba, P. oblongata n. sp., two isolates (W51C#4 & W51C#5) were also assigned to a new species of Platyamoeba, P. contorta n. sp., and one isolate (S-241) was a novel psychrophilic gymnamoeba Vermistella antarctica n. gen. n. sp. Molecular and morphological results revealed that V. antarctica was not related to any described family of gymnamoebae. Strains S-205, W51C#4, and W51C#5 were capable of locomotion at room temperature, while strains SL-200, S-131-2, W4-3, and S-241 exhibited locomotion only below approximately 10 degrees C. Our results imply that the Antarctic environment is host both to cosmopolitan gymnamoebae that have acquired adaptations for existence at low environmental temperature and to apparently novel psychrophilic amoebae described here for the first time.

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mentioning

confidence: 99%

A Description of Seven Antarctic Marine Gymnamoebae Including a New Subspecies, Two New Species and a New Genus: Neoparamoeba aestuarina antarctica n. subsp., Platyamoeba oblongata n. sp., Platyamoeba contorta n. sp. and Vermistella antarctica n. gen. n. sp.

Moran

Anderson

Dennett

et al. 2007

J Eukaryotic Microbiology

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“…A number of vesicular regions within the cytoplasm of an Arctic Paramoeba sp. isolate were identified by TEM and it was suggested that these may be cryoprotein manufacturing centres (Rogerson et al 1992). Similar regions were found in the Metachaos sp.…”

mentioning

confidence: 58%

Growth and consumption rates of bacterivorous Antarctic naked marine amoebae

Mayes¹,

Rogerson²,

Marchant³

et al. 1997

Mar. Ecol. Prog. Ser.

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This IS the first study to consider the growth and feeding rates of naked marine amoebae at temperatures below 5°C. Specific growth rates were calculated for 5 Antarctic isolates, Platyamoeba australis, A4ayorella sp., Metachaos sp., Vannella sp., and an unidentified vahlkampfiid amoeba. Growth rates ranged between 0.08 X 10-l and 2.9 X 10-' h" corresponding to generation times of 866.2 and 23.9 h, respectively. All isolates grew at temperatures down to -2OC and showed optimal growth at 2°C although one species grew best at 0°C. A direct counting method was used to estimate bacterial feeding rates of P australjs and the vahlkampfiid amoeba. Rates varled as a function of prey denslty but were largely independent of temperature. Overall, amoebae consumed between 0.5 and 66.4 bacteria amoeba-' h-', equivalent to 0.3 and 38.0 pg C cell-' h-' Growth efficiencies estimated for these 2 amoebae were low, typically < 2 % , suggesting that amoebae incur high energetic costs for life at low temperatures.

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An immunofluorescence study on lectin binding sites in gametes of Ectocarpus siliculosus (Ectocarpales, Phaeophyceae)

Maier

Schmid

1995

Phycological Research

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SUMMARY The distribution of binding sites for the lectins WGA, DSA. RCA I, PNA, AAA, MAA. SNA, GNA. and Con A in gametes of both sexes of the brown alga, Ectocarpus siliculosus (Dillw.) Lyngbye, was investigated by fluorescence microscopy. Digoxigenin‐conjugated lectins and an FITC‐anti‐digoxigenin antibody were used as a high sensitivity detection system. Organelles and other distinct cellular domains could be distinguished by their binding specificities. Glycoconjugates associated with one flagellum were found to be associated with the axoneme by lectin binding to isolated flagellar apparatuses. In addition, changes in the distribution of carbohydrate epitopes during the attachment of gametes to the substratum were revealed by differential lectin binding.

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Lectin Binding Sites in Marine Amoebae

Cited by 3 publications

References 22 publications

A Description of Seven Antarctic Marine Gymnamoebae Including a New Subspecies, Two New Species and a New Genus: Neoparamoeba aestuarina antarctica n. subsp., Platyamoeba oblongata n. sp., Platyamoeba contorta n. sp. and Vermistella antarctica n. gen. n. sp.

A Description of Seven Antarctic Marine Gymnamoebae Including a New Subspecies, Two New Species and a New Genus: Neoparamoeba aestuarina antarctica n. subsp., Platyamoeba oblongata n. sp., Platyamoeba contorta n. sp. and Vermistella antarctica n. gen. n. sp.

Growth and consumption rates of bacterivorous Antarctic naked marine amoebae

An immunofluorescence study on lectin binding sites in gametes of Ectocarpus siliculosus (Ectocarpales, Phaeophyceae)

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