Lectin binding to the human retina

Söderström, Karl‐Ove

doi:10.1002/ar.1092200214

Cited by 14 publications

(10 citation statements)

References 28 publications

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“…In addition, fluorescein isothiocyanate‐conjugated peanut ( Arachis hypogea ) agglutin (PNA; Sigma, St Louis, MO, USA) was employed to probe for the presence of cone cells at the LM level (Blanks & Johnson, 1983; Söderström, 1988; Röhlich et al ., 1989; Lukáts et al ., 2002).…”

Section: Methodsmentioning

confidence: 99%

The eye of the african mole‐ratCryptomys anselli: to see or not to see?

Cernuda-Cernuda

Garcı́a-Fernández

Gordijn

et al. 2003

Eur J of Neuroscience

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In an attempt to clarify its possible physiological role, we studied the eye of the Zambian mole rat Cryptomys anselli by light, electron and confocal microscopy using conventional staining as well as immunolabelling with rod and cone cell markers. The small eyes of Cryptomys are located superficially and display all features typical of sighted animals: iris, pupil and well-developed lens, separating the anterior chamber and the vitreous. The retina shows a well stratified organization and the folds described in blind subterranean or nocturnal mammals were not observed. The major population of the photoreceptor cells in the Cryptomys retina consists of rod cells, again with a morphology quite similar to that found in sighted animals. The relatively short outer segments contain numerous well-stacked disks and show a strong rod-opsin as well as transducin immunoreaction. Synapses were evident in the spherules, the round basal processes of the rod cell, but they lacked the precise organization reported for sighted mammals. Cone cells were present as well, as indicated by peanut lectin staining, but no immunolabelling with polyclonal M/L-opsin antisera was detectable. The presence of cone cells was also suggested by some basal processes at the outer plexiform layer which displayed several synaptic active sites and irregular contours. While the other retinal layers also showed an organization typical of sighted mammals, there were signs of less tightly preserved morphology as well. Displaced rods and amacrine and/or ganglion cells were observed, and some sparse rod spherules penetrated into the inner nuclear layer. A major reduction was observed in the number of ganglion cells, estimated from the number of axons in the optic nerve, that was very low (approximately 1000 per retina on average) relative to sighted mammals. The data we have suggest a slow, ongoing loss of cells with ageing. Apoptotic nuclei, mainly corresponding to photoreceptor cells and ganglion cells, were detected in young individuals, and an overall reduction in the thickness of the retina was observed in older animals. The morphological data presented here allow some first speculations on the physiological role of the Cryptomys eye and will hopefully trigger detailed studies on the chronobiology and the anatomy of the retinal projections and of the visual cortex of this remarkable species.

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Section: Methodsmentioning

confidence: 99%

The eye of the african mole‐ratCryptomys anselli: to see or not to see?

Cernuda-Cernuda

Garcı́a-Fernández

Gordijn

et al. 2003

Eur J of Neuroscience

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“…This OX-42 antibody is suitable for pineal gland staining because the predominant type of microglia in this tissue expresses complement type 3 receptors [43]. WGA was used as a pinealocyte marker because it labels photoreceptors [44, 45], and pinealocytes are modified photoreceptor cells [46, 47]. This technique proved to be efficient in labeling these pineal cells (Figure 1).…”

Section: Resultsmentioning

confidence: 99%

Modulation of Pineal Melatonin Synthesis by Glutamate Involves Paracrine Interactions between Pinealocytes and Astrocytes through NF-κB Activation

Villela

Atherino

Lima

et al. 2013

BioMed Research International

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The glutamatergic modulation of melatonin synthesis is well known, along with the importance of astrocytes in mediating glutamatergic signaling in the central nervous system. Pinealocytes and astrocytes are the main cell types in the pineal gland. The objective of this work was to investigate the interactions between astrocytes and pinealocytes as a part of the glutamate inhibitory effect on melatonin synthesis. Rat pinealocytes isolated or in coculture with astrocytes were incubated with glutamate in the presence of norepinephrine, and the melatonin content, was quantified. The expression of glutamate receptors, the intracellular calcium content and the NF-κB activation were analyzed in astrocytes and pinealocytes. TNF-α's possible mediation of the effect of glutamate was also investigated. The results showed that glutamate's inhibitory effect on melatonin synthesis involves interactions between astrocytes and pinealocytes, possibly through the release of TNF-α. Moreover, the activation of the astrocytic NF-κB seems to be a necessary step. In astrocytes and pinealocytes, AMPA, NMDA, and group I metabotropic glutamate receptors were observed, as well as the intracellular calcium elevation. In conclusion, there is evidence that the modulation of melatonin synthesis by glutamate involves paracrine interactions between pinealocytes and astrocytes through the activation of the astrocytic NF-κB transcription factor and possibly by subsequent TNF-α release.

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“…61 Their was also found in human, 40 in pig and cat and in rabbit retina. 22 These observations are different to our results.…”

Section: Articlementioning

confidence: 94%

“…40 However, there are some differences in the staining pattern of PNA and UEA-I that may result from species differences or from variation in tissue preparation, which are known to affect lectin histochemistry. 41,42 In my study, normal donkey retinal pigmented epithelium showed lectin binding sites for Con A, WGA, VVA and PNA.…”

Section: Articlementioning

confidence: 99%

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Lectin binding in normal donkey eyeball

Aly

2013

Vet Sci Develop

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In the present study, the distribution of various sugar residues in the eyeball tissues of sexually mature donkey was examined by employing fluorescein isothiocyanate-conjugated lectins. Our results revealed the presence of mannose (labeled by lectins ConA), galactose (labeled by PNA, GSAI, ECA), GalNAc (labeled by SBA, VVA), and GlcNAc (labeled by WGA) residues in the donkey ocular tissues. The epithelium and stroma of the ocular tissues were labeled with mannose (ConA) and GlcNAc (WGA) binding lectins. Binding sites for WGA and PNA to the rod and cone cells of the retina were evident. The lectins Con A, WGA and GSAI are bound strongly to the endothelium of blood vessels and to smooth muscle cells of the iris. In conclusion, the findings of the present study clearly indicate that the donkey eyeball contains a wide range of glycoconjugates (bearing mannosyl, galactosyl and glucosly residues), and it lacks fucosyl residues.

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Lectin binding to the human retina

Cited by 14 publications

References 28 publications

The eye of the african mole‐ratCryptomys anselli: to see or not to see?

The eye of the african mole‐ratCryptomys anselli: to see or not to see?

Modulation of Pineal Melatonin Synthesis by Glutamate Involves Paracrine Interactions between Pinealocytes and Astrocytes through NF-κB Activation

Lectin binding in normal donkey eyeball

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