Lectin-conjugated nanotags with high SERS stability: selective probes for glycans

Abstract

Surface-enhanced Raman scattering (SERS) nanotags functionalized with lectins as the biological recognition element, can be used to target the carbohydrate portion of carbohydrate-carrying molecules (glycoconjugates). An investigation of the optical...

Surface Sialic Acid Detection of Small Extracellular Vesicles at the Single-Particle Level by Nano-Flow Cytometry

“…Lectins, known as carbohydrate-binding proteins, have been extensively utilized to elucidate the global glycome of sEV preparations. − Mass spectrometry represents another versatile platform for profiling complex glycan structures and even identifying glycosylation sites of proteins within sEV isolates. , Recently, novel approaches have emerged, including DNA assemblies combined with lectin recognition, metabolic glycan labeling, and chemoenzymatic-based strategy, for exploring protein-specific glycosylation on sEVs. − Nonetheless, the glycan analysis strategies mentioned above primarily conduct ensemble-averaged analysis of sEV glycans. It is worth noting that sEVs exhibit high heterogeneity in both protein and nucleic acid compositions, as evidenced by single-sEV technologies such as fluorescence-based microscopy imaging, droplet digital PCR (ddPCR), and nano-flow cytometry (nFCM). − Whether sEVs are uniformly glycosylated or display heterogeneous glycosignatures remains to be investigated …”