Lectin-dependent T-lymphocyte and natural killer cytotoxic deficiencies in human newborns

Lubens, Renata G.; Gard, Sherrie E.; Soderberg-Warner, Margaret; Stiehm, E. Richard

doi:10.1016/0008-8749(82)90004-1

Cited by 37 publications

(11 citation statements)

References 40 publications

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“…We confirmed the previously reported observations of markedly decreased NK lytic activity in cord blood (Lubens et al, 1982) but found no differences in the number of CD56+ NK cells or differences in NK cell subsets (CD16/CD56, CD3/CD56, or CD8/CD56) between adult and cord blood. The cord blood cells did contain slightly fewer 7/8 T cell receptor-bearing cells (3% of T cells in cord blood versus 5% in adults) which can also mediate MHC nonrestricted cytotoxicity.…”

supporting

confidence: 92%

Characterization of Cord Blood Lymphocyte Subpopulations

Keever

1993

Journal of Hematotherapy

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Human cord blood is an attractive alternative to marrow-derived stem cells for transplantation. Experiences with cord blood transplants suggest that graft-versus-host disease (GvHD) may be less readily induced, even in the face of HLA differences. However, this decreased potential for GvHD might also abrogate the graft-versus-leukemia (GvL) effects of the transplant. The GvL potential might be doubly compromised since cord blood NK activity is also decreased. We have compared alloreactivity, NK cell activity and lymphokine-activated killer cell (LAK) activity of cord blood mononuclear cells with adult mononuclear cells. We find a reduced (but not absent) alloproliferative, allostimulatory and allocytotoxic capacity of cord blood mononuclear cells. Phenotyping revealed no significant differences in the proportion of T cells in cord-versus-adult blood, but cord blood T cells were nearly all of the naive CD45RA subset. Expression of LFA-1 alpha and LFA-1 beta was normal on resting cord T cells; however, they expressed significantly less ICAM-1 (CD54) than did adult PBMC. Cord blood B cells and monocytes expressed normal levels of HLA Class II. Although no differences were found in NK cell percentages or subsets in resting cord blood, cord blood NK activity was very low. However, LAK activity was much more readily induced in cord blood as compared to adult PBMC, which could be explained in part by a higher frequency of LAK precursors (LAKp). Cord blood LAK cells were readily able to lyse fresh leukemia targets from patients with ALL, AML, and CML.(ABSTRACT TRUNCATED AT 250 WORDS)

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confidence: 92%

Characterization of Cord Blood Lymphocyte Subpopulations

Keever

1993

Journal of Hematotherapy

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“…Allogenic CTL activity by fetal blood T cells obtained at 15-22 weeks of gestation varies from no detectable activity to values similar to those of adult blood T cells. Reduced CTL activity against human or murine cell targets has been found with lectin-activated cord blood lymphocytes, particularly if purified T lymphocytes were evaluated [45][46][47]. Part of this apparent deficiency could reflect the absence of memory-type T lymphocytes in the neonatal symples, because memory CD8+ T lymphocytes kill more efficiently than antigenically naive T lymphocytes after stimulation with lectin or antibodies reactive with CD3.…”

Section: Discussionmentioning

confidence: 99%

Reduced Capacity of Neonatal Lymphocytes to Inhibit the Growth of <i>Candida albicans</i>

et al. 1998

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Opportunistic microorganisms produce significant morbidity and mortality in preterm and term infants. Because of the heightened susceptibility of infants to opportunistic fungal infections, neonatal lymphocytes were assessed for their capacity to inhibit the growth of Candida albicans. Lymphocytes from both preterm and term cord blood demonstrated significantly less effect upon C. albicans than did lymphocytes from adults. Neonatal lymphocytes of infants <32 weeks of gestation showed a marked reduction in growth inhibitory capacity compared to infants >32 weeks of gestation. Lymphocytes from female infants had a significantly greater fungal growth inhibitory capacity than did lymphocytes from male infants. These results show that neonatal lymphocytes have a reduced capacity to inhibit the growth of C. albicans. This reduced antifungal capacity may underlie the increased susceptibility of such infants to opportunistic microorganisms, like C. albicans.

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“…Fetal and neonatal NK cell activity is signi®cantly decreased compared to adults [13,28] which explains the increased neonatal susceptibility to infections. Until now, no neonatal normal values of NK activity were established.…”

Section: Introductionmentioning

confidence: 99%