Lectin extracted from Canavalia grandiflora seeds presents potential anti-inflammatory and analgesic effects

Abstract

Neutrophil migration is responsible for tissue damage observed in inflammatory diseases and is also implicated in inflammatory nociception. The use of lectins has been demonstrated to be effective in different activities including anti-inflammatory, antimicrobial, and in cancer therapy. In this study, we addressed the potential use of a lectin from Canavalia grandiflora seeds (ConGF) to control neutrophil migration and inflammatory hypernociception. Pretreatment of the animals intravenously (15 min before) with ConGF inhibited neutrophil migration to the peritoneal cavity in a dose-dependent fashion confirmed by an inhibition of rolling and adhesion of leukocytes by intravital microscopy. Another set of experiments showed that pretreatment of the animals with ConGF inhibited the mechanical hypernociception in mice induced by the i.pl. injection of carrageenan or formalin. This anti-nociceptive effect correlated with an effective blockade of neutrophil influx, as assessed by the hind paw tissue myeloperoxidase levels. Furthermore, ConGF had important inhibitory effects on the mouse carrageenan-induced paw edema. In addition, animals treated with ConGF showed inhibition of cytokines release. In conclusion, we demonstrated that the lectin ConGF inhibits neutrophil migration and mechanical inflammatory hypernociception.

Structural Studies of an Anti-Inflammatory Lectin from Canavalia boliviana Seeds in Complex with Dimannosides

“…Among them, ConM is the different one in oedema induction without NO participation, lower antinociceptive efficacy or higher efficacy and potency in vasorelaxant action. Similar to ConM, Canavalia grandiflora lectin acts as anti-inflammatory and analgesic [58] and has an oedematogenic activity in rat paw oedema model (s.c.), but has a vasorelaxant effect in endothelized rat aorta [59].…”

An anti-inflammatory lectin from Luetzelburgia auriculata seeds inhibits adhesion and rolling of leukocytes and modulates histamine and PGE2 action in acute inflammation models

“…More than a century later, numerous publications still report diverse biological events of pharmacological, immunological, or toxicological origin displayed by exogenous lectins on cell systems, or in in vivo studies [1,2]. As one example from such diversity, many plant lectins induce changes in the course of inflammatory processes in acute inflammation models [3]. Frequently, previous incubation of a lectin with the specific carbohydrate inhibitor of its hemagglutinating activity rendered the lectin unable to provoke any effect, thus confirming the involvement of the carbohydrate-binding domain in mediating the biological effect studied [4].…”

Lectin from Abelmoschus esculentus reduces zymosan-induced temporomandibular joint inflammatory hypernociception in rats via heme oxygenase-1 pathway integrity and tnf-α and il-1β suppression

“…As shown by Rivanor et al, 2014 [11], who demonstrated the anti-nociceptive and anti-inflammatory efficacy of a lectin from the green seaweed Caulerpa cupressoides (CcL) in a model of zymosan-induced TMJ inflammatory hypernociception in rats, our study strongly suggest that the efficacy of AEL, similarly to CcL, involves inhibition of TNF-α and IL-1β production. Others authors have demonstrated antiinflammatory effects of lectins, showing dependence on TNF-α and IL-1β inhibition in classical models of nociception and acute inflammation in vivo [25][26][27][28][29]. The resolution of the inflammatory response during inflammatory hyper-nociception might be attributed to the inhibition of pro-inflammatory cytokines, such TNF-α and IL-1β.…”