Lectin-Glycan-Mediated Nanoparticle Docking as a Step toward Programmable Membrane Catalysis and Adhesion in Synthetic Protocells

Mukwaya, Vincent; Zhang, Peipei; Guo, Heze; Dang-i, Auphedeous Yinme; Hu, Qiangqiang; Li, Mei; Mann, Stephen; Dou, Hongjing

doi:10.1021/acsnano.0c02127

Cited by 30 publications

(40 citation statements)

References 48 publications

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“…Recently, Mukwaya et al covalently linked the Alexa Fluor 647-labeled WGA to polystyrene-co-polyacrylic acid, obtaining the 120 nm-diameter spherical nanoparticles. 44 Their experiments demonstrated that WGA recognized a GlcNAc-containing cell membrane mimic formed by semipermeable polysaccharide-polymer microcapsules. Duan et al engineered a WGA-conjugated, matrine-loaded nanoparticle that was able to target the HT-29 colon cancer cells in vitro.…”

Section: Potential Applications Of the Nanotechnique For Targeting O-...mentioning

confidence: 99%

How Nanotechniques Could Vitalize the O-GlcNAcylation-Targeting Approach for Cancer Therapy

Yang¹,

Wang²,

Wu³

et al. 2022

IJN

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Section: Potential Applications Of the Nanotechnique For Targeting O-...mentioning

confidence: 99%

How Nanotechniques Could Vitalize the O-GlcNAcylation-Targeting Approach for Cancer Therapy

Yang¹,

Wang²,

Wu³

et al. 2022

IJN

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“…[23] Those flexible interactions open up the possibility of reversible absorption and desorption, thus enabling the controllable release of attached molecules or Reproduced with permission. [3] Copyright 2020, American Chemical Society. B) Confocal microscopy images showing a mixture of three different populations of protein-stabilized droplets, fluorescein isothiocyanate-labeled bovine serum albumin (BSA-FITC,green), Rhodamine B labeled urease (urease-RBITC, red), and ATTO 425 Acid labeled glucose oxidase (GOx-Atto425, blue); scale bar in (a,b) = 100 μm; scale bar in (c) = 20 μm.…”

Section: Docked Decoration On Membranesmentioning

confidence: 99%

Hierarchical Structures in Macromolecule‐Assembled Synthetic Cells

Zhou

Wang

et al. 2022

Macromol. Rapid Commun.

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Various models of synthetic cells have been developed as researchers have sought to explore the origins of life. Based on the fact that structural complexity is the foundation of higher‐order functions, this review focuses on hierarchical structures in synthetic cell models that are inspired by living systems, in which macromolecules are the dominant participants. The underlying advantages and functions provided by biomimetic higher‐order structures are discussed from four perspectives, including hierarchical structures in membranes, in the composite construction of membrane‐coated artificial cytoplasm, in organelle‐like subcellular compartments, as well as in synthetic cell–cell assembled synthetic tissues. In parallel, various feasible driving forces and approaches for the fabrication of such higher‐order structures are showcased. Furthermore, both the implemented and potential applications of biomimetic systems, bottom‐up biosynthesis, biomedical tissue engineering, and disease therapy are highlighted. This thriving field is gradually narrowing the gap between fundamental research and applied science.

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“…On the basis of the recognition and adhesion of protein (lectin)–polysaccharide, a new study shows that artificial cells (protocells) with a biomimetic membrane composed of polysaccharide copolymers can recognize lectins by using conjugates with lectin–glycan recognition sites as assembly units . Reversible docking of functional polymer nanoparticles on cell-like microcapsules was observed.…”

Section: Applicationsmentioning

confidence: 99%

Synthesis and Biopharmaceutical Applications of Sugar-Based Polymers: New Advances and Future Prospects

Wang

Zhang

et al. 2021

ACS Biomater. Sci. Eng.

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The rapid rise in research interest in carbohydrate-based polymers is undoubtedly due to the nontoxic nature of such materials in an in vivo environment and the versatile roles that the polymers can play in cellular functions. Such polymers have served as therapeutic tools for drug delivery, including antigens, proteins, and genes, as well as diagnostic devices. Our focus in the first half of this Review is on synthetic methods based on ring-opening polymerization and enzyme-catalyzed polymerization, along with controlled radical polymerization. In the second half of this Review, sugar-based polymers are discussed on the basis of their remarkable success in competitive receptor binding, as multifunctional nanocarriers of targeting inhibitors for cancer treatment, in genome-editing delivery, in immunotherapy based on endogenous antibody recruitment, and in treatment of respiratory diseases, including influenza A. Particular emphasis is put on the synthesis and biopharmaceutical applications of sugar-based polymers published in the most recent 5 years. A noticeable attribute of carbohydrate-based polymers is that the sugar–receptor interactions can be facilitated by the cooperative effect of multiple sugar units. Their diversified topology and structures will drive the development of new synthetic strategies and bring about important applications, including coronavirus-related drug therapy.

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Lectin-Glycan-Mediated Nanoparticle Docking as a Step toward Programmable Membrane Catalysis and Adhesion in Synthetic Protocells

Cited by 30 publications

References 48 publications

How Nanotechniques Could Vitalize the O-GlcNAcylation-Targeting Approach for Cancer Therapy

How Nanotechniques Could Vitalize the O-GlcNAcylation-Targeting Approach for Cancer Therapy

Hierarchical Structures in Macromolecule‐Assembled Synthetic Cells

Synthesis and Biopharmaceutical Applications of Sugar-Based Polymers: New Advances and Future Prospects

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